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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: natedawgnow]
    #24711278 - 10/15/17 08:08 AM (6 years, 3 months ago)

I use the lighter method. Mine doesn't blow out and I have NO mold issues now but had a shit load in my SAB. I work in a basement that has drafts. I know mine blows more than what your saying and it causes me no problems so far. Also it doesn't take shit to blow away a print. Those are some small pieces of foil.


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Invisiblenatedawgnow
Rocky mountain hood rat
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: TheMadHatter420]
    #24711297 - 10/15/17 08:17 AM (6 years, 3 months ago)

I've worked with plenty of prints if front of the hood. Mine runs at about 120 or so fpm but anything up to 200 is
still good so yours probably does blow harder than mine.


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: natedawgnow]
    #24711300 - 10/15/17 08:19 AM (6 years, 3 months ago)

I only really have issues if I try to open up the print to far. The back side of the foil acts like a sail and catches the flow. Now I barely open them and slip my inoculation loop in under the foil "wrapper" and get my spores.


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InvisibleLizardWizard
GnomeGrower
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Munchauzen]
    #24711334 - 10/15/17 08:50 AM (6 years, 3 months ago)

Quote:

Munchauzen said:
Quote:

natedawgnow said:
Haha name 1 thing you can do in a SAB that can't be done in sterile flow. Literally can't think of a single thing and
I have both. On the other hand, there are TONS of things you can do in front of a flowhood that are damn near impossible
to do in a SAB.

Nice fuckin build LW! Purdy for sure. Makes me want to rebuild my cabinet :lol:



open a contaminated plate to cleanup a culture. it'll blow spores everywhere. sporenado.




Quote:

THE LAB
"Get a flow hood" and put it in a room with no drafts (unless it has a filtered intake). Use furniture that is wipeable and will not support the growth of fungi or bacteria. No carpet! A SAB will work but is not the best as it is cumbersome and difficult to maneuver things in. It is not ideal when you are wanting to ensure success on a consistent basis. A flow hood used with proper technique will be comfortable for long culture sessions and keep the air in the room clean keeping dust and shit from collecting on plates, equipment, table tops, etc. A flow hood should always be left on in a lab however you may turn it off momentarily to transfer mycelium away from sporulating contaminates. The trick to performing transfers in the open air is to do it in a sanitary room with filtered air(off while performing transfers) and never place your hands or head over the culturing media while performing the act. Proper technique for this with a petri dish is to lift the lid straight up and off the dish and use your sterilized instrument to perform the transfer. The lid should never be set aside or moved anywhere but a few centimeters straight above the media. Use antibiotics to help with cleaning up the culture. To clean up with antibiotics that cannot be sterilized use a syringe filter to clean them before introducing it to cooled media.
By lipa




And a print on foil shouldn't be blown away by the hood even if the foil piece is only a half an inch square. If you are letting your filter push that much air, you're not helping yourself in terms of contams. The filter isn't infallible, it's 99,95% effective, meaning if you push more air through, more contams are going to pass the filter in a given timespan. Drafts will be a problem, but that's easily fixed by having walls to the sides of the airflow.

Thanks for the compliments guys!

And here's Roger's take on the 90° Bic lighter bend... I find a 90° bend to be blowing a bit hard as well, and could easily imagine foil blowing away at that speed. It will also heat up the fan more, I've noticed after some 4 hours of running it, it can easily add a few degrees to the room, and a higher airflow means the motor gives off more heat.


--------------------
The best things in life
can be smelled on one's fingers.


Edited by LizardWizard (10/15/17 09:01 AM)


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LizardWizard]
    #24711351 - 10/15/17 09:01 AM (6 years, 3 months ago)

Well it does pass the lighter test, but I do intend on getting some more filters to choke it down. The only blower I could get my hands on was a 4 speed 3/4hp. I use a speed control to run it at about 50%. I don't like the way it sounds turning it below that. But hey I have not had any mold so far, and couldn't get away from it in the SAB. Things have at least improved. Done the road I intend on building a better one and using this one as a room scrubber. Also, lucky for me, mine was tested at 1900CFM. It is a big ass nuclear grade HEPA. 99.99% at 1900CFM


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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/07/15
Posts: 13,692
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: TheMadHatter420]
    #24711356 - 10/15/17 09:05 AM (6 years, 3 months ago)

Cool! I use one of these dimmers. Costs under 5 bucks.

BTW, what are you angry about?


--------------------
The best things in life
can be smelled on one's fingers.


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InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LizardWizard]
    #24711364 - 10/15/17 09:11 AM (6 years, 3 months ago)

Quote:

LizardWizard said:
Quote:

Munchauzen said:
Quote:

natedawgnow said:
Haha name 1 thing you can do in a SAB that can't be done in sterile flow. Literally can't think of a single thing and
I have both. On the other hand, there are TONS of things you can do in front of a flowhood that are damn near impossible
to do in a SAB.

Nice fuckin build LW! Purdy for sure. Makes me want to rebuild my cabinet :lol:



open a contaminated plate to cleanup a culture. it'll blow spores everywhere. sporenado.




Quote:

THE LAB
"Get a flow hood" and put it in a room with no drafts (unless it has a filtered intake). Use furniture that is wipeable and will not support the growth of fungi or bacteria. No carpet! A SAB will work but is not the best as it is cumbersome and difficult to maneuver things in. It is not ideal when you are wanting to ensure success on a consistent basis. A flow hood used with proper technique will be comfortable for long culture sessions and keep the air in the room clean keeping dust and shit from collecting on plates, equipment, table tops, etc. A flow hood should always be left on in a lab however you may turn it off momentarily to transfer mycelium away from sporulating contaminates. The trick to performing transfers in the open air is to do it in a sanitary room with filtered air(off while performing transfers) and never place your hands or head over the culturing media while performing the act. Proper technique for this with a petri dish is to lift the lid straight up and off the dish and use your sterilized instrument to perform the transfer. The lid should never be set aside or moved anywhere but a few centimeters straight above the media. Use antibiotics to help with cleaning up the culture. To clean up with antibiotics that cannot be sterilized use a syringe filter to clean them before introducing it to cooled media.
By lipa







the question was what cant be done in front of a sterile flow. if you turn the hood off, its no longer in the sterile flow. like roger states, its now an open air procedure. and I feel like the very next sentence is equally as important to illustrate the logistics of actually doing the former sentence. I know some folks have labs, but most of us don't have the luxury of a filtered sanitary culture work room.

like I said, both have their pros and cons.

its early and I need to smoke and make some coffee. sorry if im being argumentative. im gonna go out for a walk and see if i can find some reishi to clone. im interested in making some more tinctures for my blood pressure.


Edited by Munchauzen (10/15/17 09:17 AM)


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Munchauzen]
    #24711373 - 10/15/17 09:15 AM (6 years, 3 months ago)

You are right. I hung onto my sab for taking spore prints, so I can cover the arm holes and let them chill in there over night. Also you never know when a blower might crap out on you. Better to have a plan B when transfers need to be taken.


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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Munchauzen]
    #24711379 - 10/15/17 09:19 AM (6 years, 3 months ago)

It's not Roger, it's lipa (or is his name also Roger?).

Yes, indeed, it's now an open air procedure, but the open air in which the procedure is done has still been treated with the flowhood, hence, it is still a procedure in front of the hood, or at the very least in a room with air filtered by the hood. So I'd still call it a use of the flowhood, and therefore would still call a flowhood usable for cleaning up from sporulating contaminations. Though technically, it's not in a sterile flow at that moment.

And no, I don't mind the argument. I'm taking reishi as well, though not for blood pressure. Wildharvested mine, though part of it  was harvested by a fellow Shroomerite :p.


--------------------
The best things in life
can be smelled on one's fingers.


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InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LizardWizard]
    #24711402 - 10/15/17 09:30 AM (6 years, 3 months ago)

Sweet hood!  :dancer:


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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


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Registered: 01/07/15
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Loc: the parking lot
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Mycolorado]
    #24711409 - 10/15/17 09:33 AM (6 years, 3 months ago)

Thanks!


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The best things in life
can be smelled on one's fingers.


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OfflineFrank Zappotecorum
Smooth-Brained Mycophagist
I'm a teapot User Gallery


Registered: 01/01/17
Posts: 138
Loc: Scamdinavia
Last seen: 8 months, 21 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LizardWizard]
    #24711605 - 10/15/17 10:54 AM (6 years, 3 months ago)

My confession(s): I know how to do agar work fairly acceptably but resort to MS inoculations because I somehow think that will work better in my SAB despite all contrary evidence. I have Jars made/'noced on the 8th that show no signs of anything...Is there such thing as too much gypsum? I suppose the definition of cluelessness is when you understand things in theory and then blatantly disregard them when you actually try to do something:tongue:.


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What are all these leaves doing in my mouth?
I ain't got a lot but peep my Trade list


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Offlinehalfforothers
tattooguy


Registered: 08/14/16
Posts: 4
Loc: Moon Palace
Last seen: 5 years, 8 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Frank Zappotecorum]
    #24711788 - 10/15/17 12:24 PM (6 years, 3 months ago)

First post:
Hello Shroomery.
I love this place. Thank you all for all of the insight that this community provides. Its what I wish for more in American society, free exchange of information that people can use to better their lives. If we shared a bit more and guarded less with less hopes of gains of personal reputation and profit, I believe we'd be better off.
Props to all of you, for sharing what you've learned and experienced.

I confess to being intrigued by mushrooms since my first psychedelic experience in 2005 (edible, medicinal, magical), and i don't know why I'm so drawn to them... maybe because i'm worried for the natural world. 

I walk in the woods just to look at all of the colonies. The forest is beautifully flooded today, and the wood is being eaten up, recycled, sacrament for the death kingdom.

I confess to having new-agey, unfounded by physics, ideas.

I confess that after my noobery subsides (sometime in the distant future), I hope to contribute and give back to this community.


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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


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Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: halfforothers]
    #24712110 - 10/15/17 03:10 PM (6 years, 3 months ago)

Welcome to the Shroomery!

Being drawn to the myco world can save your life. In multiple ways even if you're misfortunate enough...

New-Agey ideas? Gotta watch out with that stuff, it brings bad luck!

I get my share of bad luck too though.


--------------------
The best things in life
can be smelled on one's fingers.


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Invisibleeatyualive
Eat's You Alive :)
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Registered: 08/17/01
Posts: 19,026
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LizardWizard]
    #24712220 - 10/15/17 03:52 PM (6 years, 3 months ago)

i confess, i am contemplating doing late night myco work for fun tonight.


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Offlinehalfforothers
tattooguy


Registered: 08/14/16
Posts: 4
Loc: Moon Palace
Last seen: 5 years, 8 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: eatyualive]
    #24712304 - 10/15/17 04:22 PM (6 years, 3 months ago)

Thanks, LizardWizard!
yeah, new-agey ideas like fasting for periods of time (not too long, usually just a day or 2) to reach different creative spaces, i think it does but i have no idea how or why chemically and to what extent its just a placebo i don't know. Im a visual artist, and think a lot about the realms i've visited on the fruits, sometimes the patters/languages/structures seem so concise that i wonder if i can do my best to render them visually if myself or others can make any sense of them or if there are common elements... and wondering how real are they, and how important are they? could just be a composite of things i think/read about. yeah maybe bad luck.

I confess, yes, I suck at using computers and this is the first board i'm posting to. 2017 trying to get with it.

also y'alls gifs crack me the fuck up. sweet jeezus


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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


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Registered: 01/07/15
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Loc: the parking lot
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: halfforothers]
    #24712318 - 10/15/17 04:31 PM (6 years, 3 months ago)

Did you know the gifs were all made by members?

Computers suck at me as well. They have more trouble with me the more advanced I get.

Fasting for a few days can be very healthy, if you don't do it too often.


--------------------
The best things in life
can be smelled on one's fingers.


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Offlinehalfforothers
tattooguy


Registered: 08/14/16
Posts: 4
Loc: Moon Palace
Last seen: 5 years, 8 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LizardWizard]
    #24712573 - 10/15/17 06:25 PM (6 years, 3 months ago)

I didn't know that, it makes the gifs even better. that shits hilarious.
With the fasting I do it once every few months when i feel like the sediment in my mind/body needs a little shaking. I totally believe that fasting can be healthy, probably somewhat detoxifying, it actually feels great after I get past the 16-18hr hump and pretty much everything digesting has passed (I do drink water tho). It feels like motions are easier and strong in my body and mentally clear and productive. I should probably do it again soon heh.


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InvisibleJosex
#cheat_code
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: halfforothers] * 2
    #24712836 - 10/15/17 08:14 PM (6 years, 3 months ago)

I confess this is my first day off after having finished my contract at work. It's been a tough summer with just one day off a week (dedicated to agar), unbearably hot, very little sleep, having to drive long distances to work and long hours of stressful job. 8 months of doing shit I don't like, finished now, and that feels so mushy! :awegroove:

To celebrate, I fucked today a delicious 22 yrs old pussy, been talking to her by phone all summer but could not date her cos of my job. Eating pussy feels mushy too!


I confess I've been working with an Orissa India culture for months, transferring, narrowing down genetics, only to discover it's crap. Transferred wedges from agar to pf-tek pucks, just to get some clones and the fruits never developed past the pin stage. I took some clones nonetheless, but I don't think I'll be wasting my time with them. Here's one of the multiple pf-tek pucks I inoculated:

Pins are shrivelling already, just decided to take a pic to share it here.

I did an LC with this culture, did 3 tubs and several shoeboxes from it. They colonized the sub at a reasonable speed but then went without doing nothing for like 3 weeks after full colonization, then got what it looked like a good pinset, and again they never developed :shrug:
Trashed all tubs and shoeboxes except one, just because I was curious to see what it will do. This is the shoebox, I just took the pic:

Spawned on September 15th, pins been like that for more than one week. This is a first for me, never had something similar happening before. I guess shit genetics exist indeed, but it's really dissapointing after all the agar work that I did.

As for now I'm really rooting for my "APEX", some interesting clones that are a cross of APE and a mystery cube. Shit looks amazing on agar and I hope they'll behave.

I got some LC's from these cultures, nearly ready:


I confess I have not tripped in months but today is the fucking day :rockon:


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OfflineSpotter
Making you all look good


Registered: 05/25/17
Posts: 712
Loc: True North Flag
Last seen: 2 months, 3 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Josex] * 1
    #24713129 - 10/15/17 10:21 PM (6 years, 3 months ago)

I confess, I just got my credit card bill. Looks like my new hobby is actually just buying mycology stuff. Not so much growing  mushrooms.


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Its all about the Hericium erinaceus yo.
Spotters Resume


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