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oontribe


Registered: 01/14/15
Posts: 3,570
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: mynakedrat]
#24380569 - 06/05/17 07:09 PM (6 years, 7 months ago) |
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Thailand - koh samui
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Kingdeviluke
Stranger

Registered: 04/26/17
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Lobi]
#24381209 - 06/05/17 11:37 PM (6 years, 7 months ago) |
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Quote:
Lobi said: Liquid Culture is dope if you have everything set up for it. You can do it with just a mycojar but.. I'd recommend having it set up like this and having either a tall SAB or flowhood to work with. Otherwise it's a bitch and honestly not worth it.
 I mainly use LC for reishi and sending cultures out.
Tek it for us. There's got to be the perfect way for lc. I spent 2 hours sucking out lc last night and on the last syringle the needle broke off a 60 ml chance.
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tryptkaloids
Learner



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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kingdeviluke]
#24381223 - 06/05/17 11:49 PM (6 years, 7 months ago) |
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this lid design is way over thought, you probably don't even need to filter anything... there should be enough oxygen in the jar... I use unmodded plastic lids exclusively. try Pastywhyte's EzLC
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: tryptkaloids]
#24381235 - 06/05/17 11:56 PM (6 years, 7 months ago) |
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I had bad luck with contamination using unmodified plastic lids.
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Lobi
Bushido



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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: tryptkaloids] 1
#24381236 - 06/05/17 11:56 PM (6 years, 7 months ago) |
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The lid design is dope and not really over thought. The whatman filter is strictly and entirely for GE when you draw the liquid up(if you read my original post on it.
The water has o2 and so does the jar as you stated. But pressure is a thing and if you can't draw inward pressure you can't draw out(if that makes sense.
With the whatman filter not being rtv'd to the lid it's detachable. So you can shake the living fuck out of it, flame the needle, and put it back for when you need to draw.
Ez LC is cool to an extent. But when you are doing multiple varieties and sending mass cultures out, or working with muda bottles; etc. It's useful to do this method to have more easily accessible lc.
I can pull 50 syringes in under 5 minutes with this method without rushing and without fuss.
If you aren't using syringes and you only need 20-50cc of it, I'd recommend pastys EZlc as it's probably less work.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Lobi]
#24381338 - 06/06/17 01:24 AM (6 years, 7 months ago) |
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That's a sweet idea Lobi I am definitely going to try this out. I've been meaning to mess around with LC more lately anyways.
What are those silicon pucks? Did you make those? Are they just uncommon looking injection ports you bought?
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Lobi
Bushido



Registered: 02/06/16
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: d0urd3n]
#24381370 - 06/06/17 01:57 AM (6 years, 7 months ago) |
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It is just RTV silicone that I squished between two small pieces of wax paper to make them flat. It's easier to penetrate that way and to tell when it's time to replace them.
I'll write a tek on it in GM&M as i feel it applies more there. Cubes don't really need to be put to LC. By the time the LC is ready to go you could have several masters started with the same inoculant. Then count in loading up the LC, recovery time and then colonization. By that time the masters would be done and you could expand them without compromising the water content of your spawn; doing any extra steps; or using recourses. 
But if you're doing an LC in a jar I don't think theirs a better way to do the lids. (I've thought about adding a hook for pulling the lid off when it latches though)
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TheMadHatter420]
#24381447 - 06/06/17 04:07 AM (6 years, 7 months ago) |
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Quote:
TheMadHatter420 said: I had bad luck with contamination using unmodified plastic lids.
User error
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kenetic]
#24381458 - 06/06/17 04:26 AM (6 years, 7 months ago) |
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7/10 jars using modded plastic lids sfds and all that uninnoculated contamed after 2~ weekz. The contam clearly came from the lid. Could not b user error.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (06/06/17 04:26 AM)
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Kingdeviluke
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Registered: 04/26/17
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: van hatton]
#24381468 - 06/06/17 04:47 AM (6 years, 7 months ago) |
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Quote:
van hatton said: 7/10 jars using modded plastic lids sfds and all that uninnoculated contamed after 2~ weekz. The contam clearly came from the lid. Could not b user error.
Agreed.
Lc is always faster you can turn 2ml of master lc each into qt full of lc and knock up 300 jars with 2ml each of that secondly lc. 10 days those jars are done with 10 to 1 g2g you got 3000 jars of spawn . also you really don't need to do g2g if you just make 20 secondly lc jars. Even if you start the same plate into 10 jars and only takes two weeks then g2g to 100 jars in 7 days then again in 7 days to 1000 jars in a months time and my master lc takes that long to colonized a qt of lc. But the 2 months mark lc wins to everything besides slurry. I confess I'm high on myc tea
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kingdeviluke]
#24381484 - 06/06/17 05:03 AM (6 years, 7 months ago) |
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I m8ssed the part about lc. Sorry
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kenetic]
#24381509 - 06/06/17 05:32 AM (6 years, 7 months ago) |
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My part is for grain not even lc I wouldn't do it unless I had the ones meant for pcing
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: van hatton] 1
#24381557 - 06/06/17 06:21 AM (6 years, 7 months ago) |
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I just used the unmodded lids from target. Keep them loose when you pc.

-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kenetic]
#24381585 - 06/06/17 06:52 AM (6 years, 7 months ago) |
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Same exact ones 7/10 still contamed.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kenetic]
#24381791 - 06/06/17 09:05 AM (6 years, 7 months ago) |
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Quote:
Kenetic said:
Quote:
TheMadHatter420 said: I had bad luck with contamination using unmodified plastic lids.
User error
The contamination would always happen after swirling the jar. The plastic lids were letting something into my culture. Switched to metal lids that I had made for LC and never got a contamination from swirling again. I do blame the plastic lids as they would leak solution if swirled to hard.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Kenetic]
#24381799 - 06/06/17 09:08 AM (6 years, 7 months ago) |
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Quote:
Kenetic said: I just used the unmodded lids from target. Keep them loose when you pc.


Now I do this for my grain jars and cakes. I don't have any issues with them as they are just left alone sitting.
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Lobi
Bushido



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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TheMadHatter420]
#24381844 - 06/06/17 09:29 AM (6 years, 7 months ago) |
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Honestly do not think it was user error. Those plastic lids do not have anything to seal the jar air tight. But..
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Lobi]
#24381910 - 06/06/17 09:57 AM (6 years, 7 months ago) |
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I didnt realize you guys were talking about lc
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Lobi]
#24381919 - 06/06/17 10:01 AM (6 years, 7 months ago) |
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Since I saw pinpornproducer using micro pore tape as jar filter, I started doing it. I can use my old metal lids this way. Just a 1/4 inch hole and 3-4 lays of tape. Mostly using it on cakes as I have a bunch of unmodified plastic lids as well as a bunch of metal lids with syringe filters in them, scored a good deal a while back.
I actually made a jar lids tek for it in my journal. It is more aimed at an improved PF tek lid, but less work and you got the grain jar lid. Figured it would help out noobs waning better lids but not spending alit of $. Figured if we can use it for pasty plates we are good for jar lids.
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Germs
Space Force


Registered: 06/26/11
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Loc: Texas
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TheMadHatter420]
#24381962 - 06/06/17 10:16 AM (6 years, 7 months ago) |
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So far I've done polyfill lids and tyvek with 2-3 pieces micropore on a dozen or so jars, haven't noticed any difference. If anything I like the tyvek and mp lids better bc the polyfill gets stuck to some of the grains when I shake them really hard
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