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OfflineA.k.aM
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doctor Mario]
    #26946923 - 09/20/20 09:31 PM (3 years, 4 months ago)

The whole thing??!

You need wedges in your life.

Did you have more plates?


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LAGM2020


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InvisiblePastywhyteMDiscord
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: A.k.a]
    #26946940 - 09/20/20 09:40 PM (3 years, 4 months ago)

If I’m doing wedges to grain nothing beats the tic tac toe drop IMO. It’s nearly impossible to fuck up completely. If a few wedges fall on the floor of the SAB you don’t care cause 6 still went into the grain. No scalpel needed to transfer to the jar, just a few quick cuts on the agar itself. Easy af.

https://www.shroomery.org/forums/showflat.php/Number/22601023


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Offlinetryptkaloids
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Pastywhyte]
    #26946951 - 09/20/20 09:51 PM (3 years, 4 months ago)

:whathesaid: I use regular petris for cleaning and pastys for inoculation and germination


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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InvisibleDoctor Mario
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: tryptkaloids]
    #26947032 - 09/20/20 11:12 PM (3 years, 4 months ago)

Quote:

A.k.a said:
The whole thing??!

You need wedges in your life.

Did you have more plates?




I've got more plates coming along but this one was beautiful. Nice and rhyzo and ready to rock. I did 18 transfers tonight as well. The other plates arent quite ready to go yet.

Quote:

Pastywhyte said:
If I’m doing wedges to grain nothing beats the tic tac toe drop IMO. It’s nearly impossible to fuck up completely. If a few wedges fall on the floor of the SAB you don’t care cause 6 still went into the grain. No scalpel needed to transfer to the jar, just a few quick cuts on the agar itself. Easy af.

https://www.shroomery.org/forums/showflat.php/Number/22601023




I will definitely be doing this from now on. Lesson learned.

Quote:

tryptkaloids said:
:whathesaid: I use regular petris for cleaning and pastys for inoculation and germination




Tomorrow is the day I buy some mini rounds. I went straight to pouring and am now regretting that decision. I cant see getting wedges out of a plate without using a tool and the tic tac toe method with a pasty is more than ideal. You could always tic tac toe a plate and then use the scalpel to get each piece out and into the jar but your 9 times more likely to catch a contam that way. I don't like those odds.


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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doctor Mario]
    #26947187 - 09/21/20 04:37 AM (3 years, 4 months ago)

I confess I got a bit overconfident in my Agar sterilization times... Had my first major fuck up after a long time.
Was PC'ing some grain jars recently and after they were done I decided to prep some agar, using the residual heat of the PC. It came up to 15psi really fast since it was still hot, so I set the timer for 15min as usual. But after few days, bam, contam city. 2 sleeves of Agar go into the trash :cryariver:


Normally the PC cycle for agar takes almost an hour including getting up to pressure and cooldown, but since this time the PC was still hot it was much less. I guess 15min is borderline and the heatup time contributes quite a bit to the sterilization. I'll go for 17psi and 25min for agar from now on.


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InvisiblebodhisattaMDiscordReddit
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doctor Mario]
    #26947194 - 09/21/20 04:46 AM (3 years, 4 months ago)

Quote:

Doctor Mario said:
I confess that I cant get oats to not burst. Ive tried Bod's methods and eats no boil method and they still burst after the PC cycle. Maybe 90 minutes is too long at 17psi. Eat suggests 90 min at 15 psi but I didnt really think there would be much difference in results. I also confess that I pc'd some oat jars last night and forgot to put aluminum foil over the jars. Poly was soaking wet. Dumped em all out and will try again in a few hours.



If your poly is stuffed correctly you don't need foil.


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InvisibleLotKid
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doctor Mario]
    #26947224 - 09/21/20 06:00 AM (3 years, 4 months ago)

Quote:

Doctor Mario said:
I confess that that tonight I was stoked to advance from agar to grain. I had a nice puck that I went to put in a grain jar and it fell off the scalpel and landed face down on a wet towel. 🤦‍♂️ So disappointing... I put in the jar anyway but I guarantee there is no way that its not gonna contam. Oh well. I should have another nice puck ready in about a week and a half.



I use a single piece about the same size as if i were transferring to another plate.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
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OfflineA.k.aM
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: LotKid]
    #26947286 - 09/21/20 06:51 AM (3 years, 4 months ago)

Yeah I used to go back and forth about whether it would be better to drop a smaller wedge now or let it grow out more first.  Now if I can get a piece of myc the size of a nickel it’s going in.


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InvisibleDoctor Mario
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: A.k.a]
    #26947381 - 09/21/20 08:17 AM (3 years, 4 months ago)

Quote:

bodhisatta said:
I wondered about that. When I opened the lid, the poly on the underside was completely dry but the top was soaking wet. I wasn't sure if that would eventually absorb to the other end of the poly and become a pathway for something to get in. I'm probably gonna switch to SFD's. Im confident that the poly is tight enough but it sucks twisting up and stuffing a bunch of lids at a time.

Quote:

LotKid said:
I use a single piece about the same size as if i were transferring to another plate.




Quote:

A.k.a said:
Yeah I used to go back and forth about whether it would be better to drop a smaller wedge now or let it grow out more first.  Now if I can get a piece of myc the size of a nickel it’s going in.




So is the idea of endospores getminating before myc can colonize a myth? I've read Bod's write up on endospore theory and quite a few other people saying if you PC properly you have nothing to worry about. I had originally planned to use dime-nickel dized pieces but I'm using oats and that jar was going to be a G2G master so I ended up deciding it would probably be better to use as big of a piece as possible.


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InvisiblePastywhyteMDiscord
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doctor Mario]
    #26947385 - 09/21/20 08:22 AM (3 years, 4 months ago)

Confession, I’m less worried about live bacillus surviving the PC cycle than I am about the potential damage the cry toxins they produce can do. Cry toxins won’t be killed by the PC and have anti fungal properties.


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InvisibleDoctor Mario
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Pastywhyte]
    #26947417 - 09/21/20 08:44 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
Confession, I’m less worried about live bacillus surviving the PC cycle than I am about the potential damage the cry toxins they produce can do. Cry toxins won’t be killed by the PC and have anti fungal properties.




Do you think you've encountered this yet? I've read some of your posts on that subject. What lead up to that point? Myc behaving weird or dying on grain?


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InvisibleJosex
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Pastywhyte]
    #26947440 - 09/21/20 09:05 AM (3 years, 4 months ago)

Have you had indications in your own grows that this cry toxin might be affecting them in a negative way? And in which way would you think this toxin may damage or hamper our grows? I've read something about cry toxin being linked to organic grain and being used as a substitute to replace chemical pesticides, but I'm not sure if this cry toxin is present only in organic grain or in all cereal grain including the ones treated with pesticides.


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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Josex]
    #26947461 - 09/21/20 09:28 AM (3 years, 4 months ago)

Cry toxins are produced by Bacillus species when they reproduce. They are the mechanism behind how these bacteria are able to kill insects. The toxin is stable until the pH drops which basically dissolves a protein sheath that encapsulates the toxin. Once activated it then can kill the insect.

Bacillus produce huge numbers of different toxins and many are specific to a certain insect species. But they have now found more general “broad spectrum” toxins and these I believe are what’s spurred the recent research into its anti fungal properties.

Organic grain often has huge amounts of Bacillus thuringiensis added to it and I have seen first hand issues. I was doing muda bottles with it once and used organic oats in the mix. Inoculated with LC and as the colony expanded it got weak as it tried to colonize the grains. Some areas died off completely while others were able to get a foothold. I believe I still have some of that grain left and once I get a little more organized I may plan a small experiment with it.


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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: LotKid]
    #26947469 - 09/21/20 09:38 AM (3 years, 4 months ago)

Quote:

LotKid said:
I use a single piece about the same size as if i were transferring to another plate.




I was even thinking of doing Josex' LC poke method but for grains this next run. I am waiting for my replacement filter for the hood and I'm not overly anxious to get back in my SAB for this shit. I'd like to minimize movements/exposure and increase my chances. Running behind schedule as it stands :facepalm:


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InvisibleMateja
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Pastywhyte]
    #26947513 - 09/21/20 10:07 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
I may plan a small experiment



I confess, this kind of approach is so sexy
:bashful:


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OfflinePsiloPsychIn
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Pastywhyte]
    #26947527 - 09/21/20 10:17 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
Quote:

Tweeq said:
I confess, Im not very optimistic about psilo research bc the way it's gonna go is with synthetic psilocybin only. Which means a couple of things like once this treatment is legalized big pharma is likely to organize a crackdown on natural psilocybin (mushrooms) to protect their patents. Mushrooms will be declared superdangerous in all likeliness and also, the other active components if mushrooms are left out of the equasion.

I sincerely hope I am dead wrong here but..




I share those fears. Any legalization will be done solely to benefit big business. Don’t expect there to be a place at the table for those of us who have skirted the law and done what’s morally right. We will be lucky to not be hunted down like dogs.




Me Three ...

"PsiloWellBin TM" coming at you, one tiny molecule changed and
"No bad trips, EVER!, side effects may include... getting fat, loss of sex drive, unpredictable rages, shooting your neighbor, suicide... FDA approved"
Yeah and mushrooms will be dangerous Schedule 1 black market alternative to be avoided at all costs, growers and distributors prosecuted to the full extent of the law!


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: PsiloPsychIn]
    #26947552 - 09/21/20 10:30 AM (3 years, 4 months ago)

Quote:

PsiloPsychIn said:
Quote:

Pastywhyte said:
Quote:

Tweeq said:
I confess, Im not very optimistic about psilo research bc the way it's gonna go is with synthetic psilocybin only. Which means a couple of things like once this treatment is legalized big pharma is likely to organize a crackdown on natural psilocybin (mushrooms) to protect their patents. Mushrooms will be declared superdangerous in all likeliness and also, the other active components if mushrooms are left out of the equasion.

I sincerely hope I am dead wrong here but..




I share those fears. Any legalization will be done solely to benefit big business. Don’t expect there to be a place at the table for those of us who have skirted the law and done what’s morally right. We will be lucky to not be hunted down like dogs.




Me Three ...





That's the trend with everything in our broken system...😢

Revolution!!!


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Offlinetryptkaloids
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Funky Monkey]
    #26947751 - 09/21/20 12:54 PM (3 years, 4 months ago)

I confess, my slant tubes  and microdose capsules won't be here till Wednesday and I can't fucking wait! I'm so stoked :rockon:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineGan
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: LotKid]
    #26947941 - 09/21/20 02:54 PM (3 years, 4 months ago)

Quote:

LotKid said:
Quote:

Doctor Mario said:
I confess that that tonight I was stoked to advance from agar to grain. I had a nice puck that I went to put in a grain jar and it fell off the scalpel and landed face down on a wet towel. 🤦‍♂️ So disappointing... I put in the jar anyway but I guarantee there is no way that its not gonna contam. Oh well. I should have another nice puck ready in about a week and a half.



I use a single piece about the same size as if i were transferring to another plate.




Interesting. I would've never thought of using a smaller piece. I guess just cause I imagine it would take so long. I might try it cause I only have a couple good plates of some Stro and I'd like to make 5-10 good master jars with them for g2g


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Offlinetryptkaloids
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Gan]
    #26947981 - 09/21/20 03:18 PM (3 years, 4 months ago)

Forgive me mushrooms for i have sinned. I would have had a couple killer 3rd flushes if I tended to these.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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