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InvisibleLotKid
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Doc9151]
    #26882462 - 08/15/20 10:32 PM (3 years, 5 months ago)

Quote:

Doc9151 said:
Quote:

jcm4620 said:
Quote:

Thomsen said:
I just found out that the last 200 petri dishes I've used have been non-sterile.
Nothing grows on the agar other than myc but no wonder my jars looks like this.






are u buying premade plates???? or are u just talking about the dishes?? then u talk about the agar so i can see it either way here so can u clarify



I don't understand what that means either,  a nonsterile plate is a plate that has not been sterilzed, did you buy antibiotic plates? if so,  don't do that or you can help create antibiotic resistant bacteria. they have to be properly disposed of.



I had plates that i bought as "sterile" but i dont go thru them fast enuff and sterile packaging only lasts so long before being comprimised. I firmly believe that my stash of plates were comprimised by time and or packaging. I kept losing more and more plates to random bacteria until out of 40 plates i'd have like 2 good ones. They'd tank before i even put anything on them. I switched up to reusable borosilicate dishes that i sterilze myself and my plates have never looked better. For me, it was worth the investment to swtich to reusable plates. That and chucking out less plastic is always cool.


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InvisibleThomsen
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: jcm4620]
    #26882556 - 08/16/20 12:17 AM (3 years, 5 months ago)

Quote:

LotKid said:
And that's why i switched to glass dishes.




I'm right on the edge :lol:


Quote:

jcm4620 said:
are u buying premade plates???? or are u just talking about the dishes?? then u talk about the agar so i can see it either way here so can u clarify




No premade plates. It's just plates that's not sterilized.
I'm guessing it's used for bacterial growth in schools and such.
I really never see any bacterial og fungi growth on the plates other than what i inoculate it with though.

But when I went to buy more online, I saw that the 90mm dishes weren't sterile so l contacted the company and sure enough they weren't. :facepalm3:


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InvisibleThomsen
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: LotKid]
    #26882569 - 08/16/20 12:28 AM (3 years, 5 months ago)

Quote:

Doc9151 said:
did you buy antibiotic plates? if so,  don't do that or you can help create antibiotic resistant bacteria. they have to be properly disposed of.




Not by choosing no and the price does not indicate that they should be treated in anyway, but the thought makes sense so I'll look into it.


Quote:

LotKid said:
(..) That and chucking out less plastic is always cool.




I agree on this. It's definitely the way to go. How do you process them? Packed in ten pcs and then pc or how do you do?


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InvisibleLizardWizard
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Thomsen]
    #26882666 - 08/16/20 02:00 AM (3 years, 5 months ago)

Dude if you don't see anything wrong with the plates you used for those jars, assuming it did start there, you better start getting better at judging your plates. Kizzle is a member who did a lot of work on recognition of infected mycelium in petri plates, so looking for his threads is a good start imho.


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InvisibleThomsen
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: LizardWizard]
    #26882719 - 08/16/20 03:09 AM (3 years, 5 months ago)

Well my thought was that only aerobic bacteria was in the plate and therefore did not grow underneath the agar when poured.
Although it would be odd if there were no bacteria on the lid of the plate.

I also changed the PC cycle to see if the problem is a too short cycle and are now waiting to see how it goes with the new bags.

I run a NTC plate when doing plates which always comes out clean.

My plates looks like this :shrug:


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InvisibleLizardWizard
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Thomsen]
    #26882740 - 08/16/20 03:40 AM (3 years, 5 months ago)

why do you think they're non sterile?

Is it possible you just got a bunch of lid fails or didn't vent the autoclave properly/ or took off pressure too soon?


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InvisibleThomsen
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: LizardWizard]
    #26882748 - 08/16/20 03:58 AM (3 years, 5 months ago)

I spoke with the vendor and they told me they only sell 90mm non-sterile plates.

Because of my last tubs that went bacteria i started reconsidering my work.


I think it could be the PC cycle (too short) or the plate right now. The SFD's used on the contaminated jars were brand new and should be closed properly.

I'm just doing the method of exclusion right now. :lol:


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InvisibleLizardWizard
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Thomsen]
    #26882752 - 08/16/20 04:03 AM (3 years, 5 months ago)

I'm not talking sfd's I'm talking the metal lid itself holding a tight seal. Here in Europe we generally use our plain jar lids for myco work but they aren't the 2 part type lids, they're simple metal lids that can be used but will start giving issues after 1, 2 or 3 runs, depending on how tight you put them on. Re-using lids from storebought jars is also something that's far from fool proof, I've noticed. It'd actually be wiser to re-use the  SFDs rather than the lids in these cases.

Back when I had issues with this I found out the seals of my lids were going to shit by, right after pressure cooking the jars, placing them in front of the hood, waiting till cooled with the hood on,  then inoculated and closed them up, then I saran wrapped half of the jars where the lid meets the seal. IIRC there was still 1 saran wrapped one going to shit but there was a clear difference, leading me to start buying new lids for myco work. They're the European lids though, and I don't really have a good grasp on how often lids fail at a seal in other lid types. The EU lids:


Inside of them showing the flimsy bent inwards bits that hold to the thread, one part of the problem why they don't hold up well (google pic, don't mind the fish):



If it's not your plates (they look awefully clean for non-sterile ones, idk why though) or the lids it could also possibly be sterilization times if you're not already up to 120 minutes, I hear it can differ between different batches of grain sometimes, though 2 hours at pressure should be sufficient for any jar filled with properly prepared grains (a wet grain prep can give issues as well IIRC), as long as you inoculate within a reasonable timeframe. Hot weather shortens that timeframe. I've read somewhere on the shr there are suppliers that will pressure cook their grains for much longer (6hrs iirc) cause they need to be able to store it, ship it, and have their customer store it again for a while, without encountering any infections.

Contamination is a bitch, and a sneaky one, and it can crawl in through many ways...

Wish you the best man, hope you pinpoint the source sooner rather than later.

You're not using antibiotic agar are  you?


Edited by LizardWizard (08/16/20 04:35 AM)


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InvisibleBph
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: LizardWizard] * 1
    #26882843 - 08/16/20 06:45 AM (3 years, 5 months ago)

I confess. I just made up 10 tubs and realized I dont own a working spray bottle. Got to go to the DG. I also confess that breaks are good. I was recently diagnosed with bipolar 1 and that made me realize why I have always been the way I am. Things come in waves for me. I get way too excited about something (like mush cult) to the extent that I pour all of myself into it, then one day I wake up and cant be bothered. Ive let whole grows go to shit cause I couldnt even pull the tubs down to check on them, and then when I do its a box of half rotten mush and dried out coir. Best advice you can get is do whatever makes you happy that day. Dont force yourself unless this is your only income in wich case you got to get your ass to work or find a new job. I took a 3 month break and couldn't be happier to be back. Cant wait to post a pic of my first flush. I also confess. I am really just babysitting spawn as my flow hood needs a new filter and my PC got turned into a gin still. But I'm excited to be the best damn babysitter I can be. Good to see all you guys. And to be honest is good to see I'm not the only one experiancing the ebb and flow of the shroomery. It makes me feel a little more normal.


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InvisibleLotKid
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Thomsen] * 1
    #26882958 - 08/16/20 08:16 AM (3 years, 5 months ago)

Quote:

Thomsen said:
Quote:

Doc9151 said:
did you buy antibiotic plates? if so,  don't do that or you can help create antibiotic resistant bacteria. they have to be properly disposed of.




Not by choosing no and the price does not indicate that they should be treated in anyway, but the thought makes sense so I'll look into it.


Quote:

LotKid said:
(..) That and chucking out less plastic is always cool.




I agree on this. It's definitely the way to go. How do you process them? Packed in ten pcs and then pc or how do you do?



I use stainless steel sleeves designed for sterilizing petri. They can be steam or dry sterilized.



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OfflinePuduwoke
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: LotKid]
    #26883208 - 08/16/20 10:37 AM (3 years, 5 months ago)

Lotkid have link where I can buy those?:begger:

Please


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InvisibleThomsen
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: LotKid]
    #26883343 - 08/16/20 11:51 AM (3 years, 5 months ago)

Quote:

LizardWizard said:
(...) they're simple metal lids that can be used but will start giving issues (...)

You're not using antibiotic agar are  you?




I use the two part lids yeah. Some are original Kilner lids and some are knok offs. So it could be a problem. Although lm seeing the same bacterial problem when I'm using bags. Hence the though of PC time being to short.

I was doing 1,5 hours when doing 10 jars at the time, and the same for the bags at one point. I've recently changed that to 2,5 hours for both methods. I always inoculate within about 12-24 hours and the grains never seem to be too wet.

And no the antibiotic agar. There's plenty growth when the seal breaks :lol:



Quote:

LotKid said:
I use stainless steel sleeves designed for sterilizing petri. They can be steam or dry sterilized.






Those are mighty sexy! Do you steam or dry sterilize them? And a link would be very nice!


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OfflineTweeq
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Thomsen]
    #26883502 - 08/16/20 01:47 PM (3 years, 5 months ago)

@lizardwizard I'm also in Europe and use the one piece metal lids too. I've been (re)using the same lids since the beginning, some 1.5 yrs ago without any problems so far..


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InvisibleThomsen
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Tweeq]
    #26883575 - 08/16/20 02:37 PM (3 years, 5 months ago)

Quote:

Tweeq said:
@lizardwizard I'm also in Europe and use the one piece metal lids too. I've been (re)using the same lids since the beginning, some 1.5 yrs ago without any problems so far..




What do you use as filtration?


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InvisibleLizardWizard
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Thomsen]
    #26883582 - 08/16/20 02:43 PM (3 years, 5 months ago)

It's not the filtration. But I do tend to twist on lids rather thight, and have noticed the little bent pieces getting out of shape quickly so maybe it's just me :shrug:


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Offlinejcm4620
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Thomsen]
    #26883662 - 08/16/20 03:52 PM (3 years, 5 months ago)

Quote:

Thomsen said:
Quote:

LotKid said:
And that's why i switched to glass dishes.




I'm right on the edge :lol:


Quote:

jcm4620 said:
are u buying premade plates???? or are u just talking about the dishes?? then u talk about the agar so i can see it either way here so can u clarify




No premade plates. It's just plates that's not sterilized.
I'm guessing it's used for bacterial growth in schools and such.
I really never see any bacterial og fungi growth on the plates other than what i inoculate it with though.

But when I went to buy more online, I saw that the 90mm dishes weren't sterile so l contacted the company and sure enough they weren't. :facepalm3:





theres your problem stop buying pre made/pre poured plates!!! and the wrong kind at that. and also for the record when you say you bought plates that werent sterile that could mean that you bought empty pitris that were not in sterile sleeves or you bought pre made/pre poured plates. to most of us that means that you bought pre made stuff meaning you didnt prepare the agar and pour them yourself but the way you worded it coulda gone either way


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Offlinejcm4620
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: jcm4620]
    #26883681 - 08/16/20 04:03 PM (3 years, 5 months ago)

lol im still confused after comin back to this but where did u get those steel sleeves at??? or did u make them ive thought about getting glass myself lately


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OfflineShroom Mafia
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: jcm4620]
    #26883996 - 08/16/20 09:16 PM (3 years, 5 months ago)

I realize this confession is most likely considered heresy and liable to get me burned at the stake for the very mention of it, but have NEVER used a glovebox, SAB or flowhood. If anything, could totally see using a flowhood eventually, but am kinda old school in how I do things and have just never had the absolute need for it. I just turn off all fans and sources of air movement, mop and clean the "lab" and proceed to rip and tear.

Most of my sessions result in over 90% success rates. However I will admit that on the rare occasion things do go bad these days, they have the tendency to go REALLY bad. Even then, nothing more than 50% bad though.

If I remember correctly though, there's is mention in The Mushroom Cultivator by Paul Stamets (realizing many here may consider that book extremely outdated by the way), something along the lines of him saying that he has seen some cultivators who have extremely primitive equipment / conditions, who can obtain much success, and seeing others with top of the line equipment / conditions not do as well as the aforementioned.

Maybe I'm just a glutton for punishment though!


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OfflineTight Lunchbox
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Shroom Mafia]
    #26884004 - 08/16/20 09:23 PM (3 years, 5 months ago)

That's interesting. Do you have any pictures of your grows?


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Offlinejcm4620
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Re: TRUE CONFESSlONS - of an amateur mycologist [Re: Tight Lunchbox] * 1
    #26884008 - 08/16/20 09:28 PM (3 years, 5 months ago)

i call bs sorry pics or it dont happen

oh and stammets is a fucking hack


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PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (08/16/20 09:29 PM)


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