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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: foragedfungus]
#26866172 - 08/06/20 10:36 AM (3 years, 5 months ago) |
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I confess that you guys should really try using only iso and drop the flame. it's not necessary. Try it it works, I haven't flamed in over a year now and any contaminants is because I screwed up and touched the sides of the jar or moved over my sterile field and not from losing the flame. I know when I make a mistake, I mark it on the jar in case I see anything wrong I'll know why.
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doc9151]
#26866465 - 08/06/20 01:13 PM (3 years, 5 months ago) |
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How do you use the ISO? Do you dip the knives or wipe them of some nice technique? I personally use neither flame nor ISO I always have a qt jar in the SAB full of sterilized knives with the lid sealed of course and properly filtered. I always hated flaming and haven't done it more than a few times lol went out and bought a bunch of knives right away. Used to put foil on the blades and pc inside open qt jars without lid and some water inside, now I just put them dry inside a jar with the lid on and throw in the PC every time I'm pcing liquids.
-------------------- Cakes inside Water Tub
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Mateja]
#26867134 - 08/06/20 07:36 PM (3 years, 5 months ago) |
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I keep two paper towels with iso, one is damp and the other is wet, but not dripping. I hold my scalpel with the damp one while wiping the knife down from the tip of the blade to the bottom of the handle without touching it with gloves, squeezing a little iso from the wet towel into the cracks of the blade where it meets the handle and proceed down the handle wiping away any excess iso with the damp towel and then wrap the knife in the damp towel until ready to use, covering the entire knife completely. I don't mean you have to squeeze out a bunch of iso into the crack, if the towel it wet it will have obvious excess on the blade and handle as you wipe. just Clean well at the junction where the blade and handle meets. It's the blade that is most important to Clean each time because the handle shouldn't ever be in contact with your work. Then main thing is to not have excess iso on the handle or blade once you are ready to work. wait a few seconds after wiping the blade for the iso to evaporate off the blade, because for alcohol to kill contaminants it has to dry, it's the drying that rupture cells killing the baddies. So, I wouldn't worry about having dripping wet towels, only wet enough to squeeze small amounts out around areas of concern like mentioned above. I use the wetter paper towel to wipe everything being careful not to expose any open jars or plates while wiping down.
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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Puduwoke
mushroom enthusiastic

Registered: 06/25/17
Posts: 1,666
Last seen: 11 hours, 1 minute
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Grimsweeper]
#26867620 - 08/07/20 03:53 AM (3 years, 5 months ago) |
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Quote:
Grimsweeper said: If my hood ever broke down and I couldn't afford to fix it I'd probably quit until I could. I can't imagine ever dicking around in a SAB again. So much prep, wiping shit down, putting all the shit away again fuck that. Flick a switch let's go to work.
How long do you let it run before starting to work? I know a lot of people say let it run for about 1 hour. I usually do only 15 because I have my flowhood in the basement of my apartment building and I'm bot allowed to have my mushrooms lab down there so I gotta do all my flowhood work very early in the morning and 4am I do not want to sit and wait 1 hour before starting to do work.. I know I could use a timer etc but wouldnt work with my situation.
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Trade List LAGM2021
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Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 7 days, 16 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Puduwoke]
#26867721 - 08/07/20 06:38 AM (3 years, 5 months ago) |
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Damn dude that's pretty ballsy. I only run it a minute or two while I'm gathering my supplies then right to work. I keep a piece of cardboard over the filter while not in use and crank my blower speed all the way up to blow any particles off that may have collected then turn it down to working speed when im ready.
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Grimsweeper]
#26868313 - 08/07/20 12:54 PM (3 years, 5 months ago) |
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Quote:
Grimsweeper said: If my hood ever broke down and I couldn't afford to fix it I'd probably quit until I could. I can't imagine ever dicking around in a SAB again. So much prep, wiping shit down, putting all the shit away again fuck that. Flick a switch let's go to work.
You say that until you need to clean up a moldy plate. A sab has its place even for someone with a flowhood
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 7 days, 16 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: tryptkaloids]
#26868452 - 08/07/20 01:59 PM (3 years, 5 months ago) |
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Yeah I get that reasoning but honestly when would you want to clean up a mold sporing plate unless you're trying to save the only genetics of a culture?
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Grimsweeper]
#26868849 - 08/07/20 06:01 PM (3 years, 5 months ago) |
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Maybe its a really good culture and you didn't make a backup/threw away the donor
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 8 days
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: tryptkaloids]
#26868851 - 08/07/20 06:04 PM (3 years, 5 months ago) |
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That's pretty much what he just said.
I suppose someone might do it simply to see if it can be done. I'd rather just swipe some new spores.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Tight Lunchbox]
#26869839 - 08/08/20 10:24 AM (3 years, 5 months ago) |
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I don't see any point in letting your flow hood run like that, turn it on wait a minute or two for it to blow off any dust on the flow side and go to work, what matters is that you are working in the airflow. any contaminants floating in the room will have to pass through the filter to get to anything in front of the flow because it can't just fall through the air and onto your work.
If you are using your flow hood to clean the air in a room you can leave it running but that's a waste of a filter.
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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foragedfungus



Registered: 09/30/13
Posts: 1,849
Loc: out there
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doc9151]
#26869878 - 08/08/20 10:56 AM (3 years, 5 months ago) |
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My hood pushes air at 400 cfm. My lab room is 10x12x8, 960 cubic feet. I'm sure the circulation around the room isn't perfect, but in some ideal situation the whole volume of air in the room could be filtered in less than 3 minutes...
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Doc9151]
#26869896 - 08/08/20 11:14 AM (3 years, 5 months ago) |
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I’ve got Trichoderm CQ on one of my APE tubs. I confess.
I was just inspecting these last midnight and there was nothing obvious showing. That white patch popped up quick af. The corner looked odd though so I had already singled this guy out for watching.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Roger Clemency]
#26869907 - 08/08/20 11:22 AM (3 years, 5 months ago) |
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I confess:
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26870131 - 08/08/20 01:42 PM (3 years, 5 months ago) |
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Grain jar broke in my hand today and sliced my hand up real nice. Lets see how the mycelium appreciates a little blood in the substrate.
Blood sacrifice = moar potency 
And seriously be careful when breaking apart grain jars. I really didn't bang it all that hard. Might have to try bike tires instead of padded furniture
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Psilosopherr]
#26870136 - 08/08/20 01:49 PM (3 years, 5 months ago) |
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Better yet, check your jars before use for stress fractures, particularly in the edge around the bottom... also check them.after sterilization and before inoculation
I confess, I've done it... a few times
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26870138 - 08/08/20 01:50 PM (3 years, 5 months ago) |
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Think I remember one guy on here saying he cut himself so bad he went to the ER 
And thanks for the tip. This brand of jar also feels a little thinner than the ones i'm used to
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Psilosopherr] 1
#26870159 - 08/08/20 02:10 PM (3 years, 5 months ago) |
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I've noticed also that round jars fair better, longer, than partially or completely squared jars, and that smaller grains (like millet, milo, WBF) crack jars in fewer cycles than oats: I suspect from compression as the pressure cycle fluffs the grain, the larger oats dont pack as tightly and thus exert less internal outward pressure maybe? Speculation, and correlation not necessarily causation...
Quote:
Mycobro420 said: I confess that. even on vacation I can't stay off the sight for one day
You've quite the journey ahead of you
I never wiped shit down for SAB work... just thoughtful hand placement, no unnecessary movement... slow and concise
Edited by Caps McGee (08/08/20 02:17 PM)
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 918
Loc: Deep inside the night
Last seen: 3 months, 20 days
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26870297 - 08/08/20 04:06 PM (3 years, 5 months ago) |
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I use a rolled up phonebook to break apart jars , works really well barely have to hit it against it
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Marmie]
#26870355 - 08/08/20 05:00 PM (3 years, 5 months ago) |
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I use my hand to break up jars. I bring the phone book in, promptly forget I have a phonebook and break up all the jars with my hand. Terrible habit. I’ve broken my share in my hand. Never needed stitches though.
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Pastywhyte] 1
#26870413 - 08/08/20 06:01 PM (3 years, 5 months ago) |
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I confess, I recently joined the shroomery Facebook page and I am just baffled; amused; confused; and a little tripped out by it. I'm paranoid about posting certain things here on the main site, but oh my goodness. What Is it that makes everyone feel so comfortable with posting grows on Facebook under their real names? I can remember a post in the newsfeed about someone posting some morels they found and were preparing to cook and within an hour the police had kicked in their door. Luckily, the Lt that showed up recognized the mushrooms and ordered the cops to leave.
Am I missing something? I don't trust Facebook not to rat me out because I know they will.
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
Edited by Doc9151 (08/08/20 06:03 PM)
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