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Voltageloss
Stranger everyday

Registered: 11/23/16
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24041277 - 01/26/17 01:20 PM (7 years, 4 days ago) |
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Thanks bud. Was having a hard time finding pics of Cyan myc on agar, especially early germination pics. Trying to build some reference. Keep it up, good work.
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Voltageloss]
#24054357 - 01/31/17 12:08 PM (6 years, 11 months ago) |
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2017-01-31
Ps. azurescens woodchips spread out to the size of a small coin and then a satellite colony of... something appeared so I did some transfers on azures and cyanescens. I took the transfers a bit ago and they've grown out, I did grab the leading edge. You requested more pics of the mycelium so here they are. 
Donors:
 cyan
 azur, the contaminations got even worse, guess the woodchips were dirty (well, they were outdoors)
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Edited by Tiamo (02/11/17 11:30 AM)
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Adas
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24054381 - 01/31/17 12:17 PM (6 years, 11 months ago) |
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These plates look fucked, man. Are you sure you don't want to wait till you have some spores?
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Adas]
#24054426 - 01/31/17 12:34 PM (6 years, 11 months ago) |
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Sorry, but what? The azurescens is obviously fucked, but there is lots of clean myc on there around those woodchips. The contamination popped up only 24 hours ago and grew that much in those 24 hours, that makes it easy to spot a contam. Obviously, I transferred the clean myc. The cyanescens is 100% clean and is from spore, if you had read the main post you would've known that and about my previous failures with agar. I streaked the plate, it didn't just grow from the middle. Also, the pink is food colouring.
Seriously, I know how to tell clean myc from contamination and these are most definitely not fucked. They have lots of healthy mycelium available for transfer. I even transfered the azures before anything sporulated.
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Edited by Tiamo (01/31/17 12:45 PM)
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Adas
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Registered: 12/22/16
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24054459 - 01/31/17 12:49 PM (6 years, 11 months ago) |
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Okay then. It's clearly an advantage that mold spores take some time to germinate, isn't it? Also, pretty brave of you to put unsterile colonized woodchips on agar. If I didn't see it I would bet my ass it couldn't work haha.
Keep us updated.
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Voltageloss
Stranger everyday

Registered: 11/23/16
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Adas]
#24054522 - 01/31/17 01:15 PM (6 years, 11 months ago) |
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Voltageloss]
#24082019 - 02/11/17 11:29 AM (6 years, 11 months ago) |
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2017-02-09
I made a blenderless LI using two agar plates (Ps. cyanescens) to 400 mL of water and used it to inoculate 8 L of spawn consisting of 80% grains, 10% woodchips, 10% saw dust. Below is one of the colonies used to make LI.
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Edited by Tiamo (02/11/17 02:02 PM)
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Adas
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Registered: 12/22/16
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24082301 - 02/11/17 01:56 PM (6 years, 11 months ago) |
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Could I ask you why you haven't used more % of woodchips in your spawn? In my humble opinion, grains should just be used as energy source to help the myc grow faster on the wood. At least that's what I do.
Really looking for your progress.
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Adas]
#24082310 - 02/11/17 02:01 PM (6 years, 11 months ago) |
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I hypothesised that the more grain spawn used, the higher the yield will be.
liloldme in his grow found that the higher spawn rate with a better energy source (i.e. grains) yielded better than a low spawn rate with a worse energy source, such as saw dust spawn or LC.
The spawn's purpose is to propagate mycelium for the main substrate. The main substrate usually has little nutritional value compared to the spawn. In this case, the main substrate will be a mix of soil, wood chips and saw dust. This does not have enough energy to produce tons of mushrooms.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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Adden

Registered: 06/04/03
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24082436 - 02/11/17 03:05 PM (6 years, 11 months ago) |
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Quote:
Tiamo said: liloldme in his grow found that the higher spawn rate with a better energy source (i.e. grains) yielded better than a low spawn rate with a worse energy source, such as saw dust spawn or LC.
The spawn's purpose is to propagate mycelium for the main substrate. The main substrate usually has little nutritional value compared to the spawn. In this case, the main substrate will be a mix of soil, wood chips and saw dust. This does not have enough energy to produce tons of mushrooms.
Yeah and with his high use of grain he better feed it more wood in those areas or it won't establish and give weaker flushes as it dies. It's my understanding he left plenty of mushrooms though to rot back into the mycelial mats. I'm worried about the areas he cased with peat coz of pH.
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Adden]
#24082445 - 02/11/17 03:09 PM (6 years, 11 months ago) |
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Adden]
#24082484 - 02/11/17 03:20 PM (6 years, 11 months ago) |
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I'm not too worried about keeping this patch for several years. I think I'll just use fresh spawn and chips and such next year again.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo] 1
#24102208 - 02/19/17 09:33 AM (6 years, 11 months ago) |
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2017-02-19
I also made a blenderless LI using one agar plate of Ps. azurescens and used it to inoculate spawn consisting of 80% rye 20% woodchips. Here is one big happy family!
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo] 1
#24119063 - 02/25/17 03:01 PM (6 years, 10 months ago) |
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2017-02-25
Cyanescens jars are almost done, they look good. I think I made a mistake with the saw dust, they don't seem to want to colonise it very well. In places they grab onto it, but it's pretty rare. Either way, shouldn't matter too much.
The azurescens jars also looking very nice.
Although I inoculated both jars with a blenderless LI I haven't seen the crazy growth I was expecting. Not sure what's up with that. I presume it's my hard agar, next time I will try soft recipe.
 cyan jar
 azur jar
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24119097 - 02/25/17 03:20 PM (6 years, 10 months ago) |
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Any idea why they don't colonize the sawdust? I've heard of it not being a good substrate before, but I would like to know the reason. Nice jars btw.
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Adas]
#24119112 - 02/25/17 03:25 PM (6 years, 10 months ago) |
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Well, I found out it was spruce wood. (Norway spruce or Picea abies)
I think that might have something to do with it, but I also read multiple sources that it should work. I used the same saw dust with Reishi mushroom and they did fine on it.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo] 1
#24180015 - 03/21/17 10:34 AM (6 years, 10 months ago) |
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Has been a long time since an update!
2017-03-21

The cyanescens patch has been laid. 
About 5 liters of spawn, into a 45 L monotub, kept feeding it wood over the weeks and eventually it was almost entirely full. I decided then it was time to bring them outside. Dug a big hole, put cardboard down in the bottom, put my last jar of spawn over the cardboard. Spread out all the wood from the tub into the hole. Covered with woodchips, few shovelfuls of dirt and mixed thoroughly.
Good times. 
Tub in progress, kept feeding it wood, cardboard (and pizza boxes lol)

Ps. azurescens spawn coming along too
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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Edited by Tiamo (04/08/17 10:22 AM)
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Adden

Registered: 06/04/03
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24181105 - 03/21/17 05:23 PM (6 years, 10 months ago) |
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Keep up the great work!
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Tiamo
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Adden] 1
#24227458 - 04/08/17 10:22 AM (6 years, 9 months ago) |
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2017-04-08
The azurescens patch has been laid next to the cyanescens patch. About 4 L of grain spawn went into this baby. I soaked them in water and shook before inoculating the patch. Kinda like making a GLC but throwing the grains in there too. I think this will benefit colonisation. Thinly cased with mix of coir/dirt (the dirt is more like clay where I live ). I want some plants/grasses in the patch, any idea how I could set that up? I don't think they're capable of growing there because the ground is pretty much solid woodchips.

I got Ps. serbica on agar but the growth is very slow. I'm thinking it might be bacterial but it doesn't look like it. Gonna make more transfers to get good growth, maybe I can get some fruits this fall.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Just_A_Noob
Breathing



Registered: 12/30/16
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Re: 2017 Outdoor Ps. cyanescens & Ps. azurescens grow western EU (Zone 8) [Re: Tiamo]
#24227465 - 04/08/17 10:26 AM (6 years, 9 months ago) |
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Go for a walk, find some tall grass from last year, grab seed tops and spread... I would think some grass would help with potency but that is only hypothetical
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