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OfflineR34P3R
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Grow Log: Worlds Worst Mycologist
    #23947767 - 12/21/16 11:51 PM (7 years, 1 month ago)

So after a discussion and a tiny bit of research I decided to grow some mushrooms. I mean it's fungi how hard could it be?

I consulted many YouTube videos and posts here. It became very apparent everything I need to know is found in the Marc Keith videos. I don't  know that he invented the techniques but that is the point of origin on the net as far as I can tell. With that said, I opted to go with rye berries and skip the cakes. I watched the videos so I know this is going to be super simple!

Attempt 1:

Grocery List:
Box of QT Jars
Pressure cooker
Alcohol and containers
Organic Rye Berries
Gypsum

I get all ingredients together
Washed rye
Soaked rye with gypsum for 24 hours
Brought to boil for 10 mins
Strained
Let steam dry with occasional mixing until it passed the tissue test
Loaded jars
Put small hole in the top  and filled with polyfill
Covered with foil
PC 15psi  for 90 mins
Let sit overnight to cool

I used a small bathroom to inoculate  that I cleaned thoroughly
I then ran an air purifier for about 1 hour
I then sprayed the air with Lysol and allowed it to settle
I inserted the needle through the polyfill
I sprayed 1cc in each jar

I placed the jars on a shelf at room temperature

I did not touch them for a week and then took them down to see how things were going. To my surprise... they weren't! Nothing had happened. No colonization of anything. I waited another week and still nothing.  Bird food they became.

Attempt 2:

Grocery List:
Micropore Tape
Sunflower Lecinthin
Coir
Pickling Lime

I repeated attempt one with the exception of the following:

I washed the rye after the soak overnight and used fresh water to boil in.
I added sunflower lecithin to the boiling berries
I boiled for 20mins instead of 10
I used micropore tape instead of polyfill
I fire sterilized between jars when I inoculated

I had given thought to what substrate and casing method to use. I opted to go with coir so I picked that stuff up as well.

I waited 10 days before checking on these jars. I pulled them down in anticipation and... nothing. Not a damn thing.  WTF is going on!

I reviewed all the steps and tried to find error or suggestions others had for nothing grows. The colonizing process seems very simple. There are only a few variables that are going to cause issues; contamination was not an issue since nothing at all was growing, substrate did not seem like it was the issue since I verified no fungicide was used in the product, bad spores which came from a known vendor so I did not immediately think that and finally water. I was not concerned about the amount of water but more the quality. We have well water which gets treated with chlorine and salt. I thought that had to be it!

Attempt 3:
Grocery List:
Distilled water...Lots of Distilled water

I repeated all steps in attempt 2 with the exception of using distilled water. I was also on syringe 2 at this point.

I once again waited 10 days and AGAIN nothing. Something is terribly wrong. I went back over my Tek and could not find any hard reasons why this is happening. I read more threads on no and stalled growth here. One thing that some people talked about was air exchange so I thought what the hell and took the lid off one of the jars. A few days later BOOM WE HAVE GROWTH but it was mold :frown: at least I now knew my jar was capable of supporting life. I came to the conclusion it had to be the spores.

Attempt 4:
Grocery List:
New spore syringes

I repeated the steps used in attempt 3 except I went back to tap water. After further research it appears the chlorine will be removed in the heating process.

Again nothing. I thought maybe it was not warm enough so I put a heat bad indirectly by the jars to heat them more. And boom we have growth ... shit... mold. Ok I am just going all out on the next one and trying every syringe.

Attempt 5
Grocery List:
Freebie!

I repeated attempt 4. The exception is that I used 2cc sprayed to the edge of the jar and not the center with 1 strain per jar. I did not use the heat pad again. More post reading on here I concluded room temp is just fine (75f here)

Again nothing. This is when I gave up on rye and pretty much decided I could not grow mushrooms. Time went by and it really bugged me that I could not get this to work. I watched all the videos again. I read many more posts here and decided to have another go. I decided to go with the noob proof cakes this time. I also opted, for some unknown reason to grow the spores on agar as well.

Attempt 6
Grocery List:
Wide mouth small jars
Verm
Perlite
Brown Rice Flower
Scale
Beakers
MEA
Petri Dishes

I followed the BF tek from the let's grow mushrooms series exactly to the letter. For the agar I mixed just shy of 5g MEA to 200ml of water. I loaded everything up together and off we went and then I noticed the silence. The PC is not making any noise! Yea the worst has happened and I boiled it dry causing a huge bow in the bottom of my PC. I figure the agar was still ok to use. So I salvaged that an poured dishes. I followed the same procedure as inculating jars but using only a couple drops of each strain per dish. I did make sure there were a little bit of visible spore clumps since the syringes were dense.  After a week no mycelium but plenty of bacteria and some mold on 2. I assume this was due to the PC failure but I am going to use distilled water next time.

Attempt 7
Grocery List;
Pressure Cooker (I went with the big boy All American this time)
Distilled water
Glass Petri dishes

This attempt was identical to 6 with exception of distilled water in place of tap for making the agar. I inoculated the cakes using the described method in the video with exception of no glove box. I waited about 5 days and checked the cakes and the dishes. No growth in either. Good news is no contamination on the agar this time. I contacted the spore vendor and explained the situation. They agreed this is an odd issue but just to be sure they are replacing my syringes! That is some outstanding customer service.

I am thinking this is not going to be my thing.... but wait there is more... So today I am cleaning up my office that had turned into a lab. Tossing the Petri dishes. I grab the jars to go clean out and I look down at the first jar I pulled out and Boom we have growth! I looked closely and yes it is nice dense white and right at the inoculation points. I check the other jars and YES all but one is growing!

So that is the grow log to this point and I am really getting hooked on this stuff :smile: Any other ideas on the rye issue would be greatly appreciated.


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Offlinewtfcrazymofo
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R] * 1
    #23947792 - 12/22/16 12:04 AM (7 years, 1 month ago)

Patience, Try to leave them on the shelf until a shake is required(30%).  Agar work should come before grain work.  MS > grain is sometimes a let down.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineMattisfat
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Re: Grow Log: Worlds Worst Mycologist [Re: wtfcrazymofo]
    #23948097 - 12/22/16 04:09 AM (7 years, 1 month ago)

Yeah it can take 2 weeks to germinate spores - you just need to play the waiting game


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OfflineTiamo
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Re: Grow Log: Worlds Worst Mycologist [Re: Mattisfat]
    #23948115 - 12/22/16 04:38 AM (7 years, 1 month ago)

Sounds to me like you didn't wait long enough. You can wait up to 2/3 weeks for spores to germinate on agar or for any growth to appear in the rye or brf cakes. The colonising part is very slow.


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
    #23948179 - 12/22/16 06:28 AM (7 years, 1 month ago)

Thanks for the responses. I admit that the last 5 day wait was being quick on the gun, but I would think that 10 days was plenty of time to see at least SOME growth No? Perhaps my expectations were a bit optimistic. I plan to try the Rye again when I can inoculate from the petri. Who knows how long that will take me to figure out lol :eek: Hopefully the rest of the cake process will go smooth.


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OfflineTiamo
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #23948210 - 12/22/16 06:58 AM (7 years, 1 month ago)

Quote:

R34P3R said:
I would think that 10 days was plenty of time to see at least SOME growth




From spore to agar, rye or brf, no, it can take a lot longer than that. From a LC or LI, definitely.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisiblemushboyMDiscord
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
    #23948271 - 12/22/16 07:48 AM (7 years, 1 month ago)

Stay away from water softeners!! They fucked with my grows something fierce.
Also dont use distilled water.

Also a bathroom is a terrible place to inject jars.

Use the spiltball link in my signature. All you need to know is within.


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: mushboy]
    #23948292 - 12/22/16 08:07 AM (7 years, 1 month ago)

Quote:

mushboy said:
Also a bathroom is a terrible place to inject jars.





From what I have seen, my bathroom is cleaner then where 95% of people are inoculating :wink: I kid. I have a spare bathroom that is only used by guests but still gets cleaned twice a week. All surfaces are solid and easy to sanitize. It is also the smallest suitable room. I understand the word bathroom does not sound sanitize'y but I assure you it was. My issue was not contamination, it was lack of any growth at all. I do think the water is playing into it somehow but I am not sure how yet.  If I continue on I will make a glove box and if I go further I will graduate to the hood... Baby steps :wink:

Thank you for the link I will have a look!


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InvisibleMad Season
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R] * 1
    #23948379 - 12/22/16 08:45 AM (7 years, 1 month ago)

:thumbdown: glove box.. :thumbup: still air box!

They take less than 5 minutes to make my friend. If your bathroom is still with the vents closed, having a still air box inside of a room that's still just adds onto your success rates.


--------------------
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Improve your sterile techniques! (A comprehensive guide to agar)
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OfflineTiamo
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
    #23948390 - 12/22/16 08:50 AM (7 years, 1 month ago)

My glovebox cost me €10 for a 130 L clear tote, I drilled with a hole saw and was done in <2 mins. You can use a coffee can heated on a stove or a sharp knife too.



Would recommend, but don't think that's your issue. Your issue is just patience. I definitely recommend going agar first and inoculating with a blenderless LI, you will notice growth much faster. Make sure you have a clean, full plate of mycelium. :yesnod:


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleMad Season
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
    #23948392 - 12/22/16 08:52 AM (7 years, 1 month ago)

Fuck the sharp knife/screwdriver method. Use scissors instead.

https://www.shroomery.org/forums/showflat.php/Number/23897456


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineTiamo
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
    #23948399 - 12/22/16 08:54 AM (7 years, 1 month ago)

I have a real sharp Spyderco knife, real nice blade and well sharpened, see my gallery. It will slice your scizzors right in half. :rofl:


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleMad Season
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
    #23948408 - 12/22/16 08:58 AM (7 years, 1 month ago)

What's funny AF is those scissors I used were already broken in half. They had no handles, as you can see by my weird way of holding them. It's so fuckin easy to cut a box up with them. Just like cutting a nice circle in paper. I really couldn't imagine doing that with a knife all that easily. Maybe you could prove me wrong on that :shrug:

And for germinating lame spores agar is the best route. Charcoal agar, sandwich teks, hot pour, etc are stuff people do to germinate shit spores


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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OfflineTiamo
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
    #23948417 - 12/22/16 09:02 AM (7 years, 1 month ago)

I have a feeling the serrated edge + heat will feel slice through it like butter, similar to scissors but I have no need for a second SAB. So not in a rush to prove it to you. Anyway, getting a bit off-topic. I'll stray away.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleMad Season
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
    #23948421 - 12/22/16 09:05 AM (7 years, 1 month ago)

I don't even have a knife like that to try xD. Broke ass over here. I do have a blunt katana tho :rofl:

And yeah I really don't think the lack of SAB is the problem with tricky spores. But agar is the shit for tricky spores IMO. There's only ever been 1 syringe I've never been able to get to germinate on agar and the various forced germination methods


--------------------
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Improve your sterile techniques! (A comprehensive guide to agar)
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
    #23948540 - 12/22/16 09:47 AM (7 years, 1 month ago)

dont use a knife. your SAB will look like mine. it works but yikes...


BACK at OP..

i didnt say your failed because of contams. i said a bathroom is a bad place to work. they have built in mold growers in the floors(toilets).

water is playing into it because softeners have salt added to the water.
salt is bad for mushrooms. i think distilled also has salt. you want a water rich in minerals. not salt.


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InvisiblebodhisattaMDiscordReddit
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Re: Grow Log: Worlds Worst Mycologist [Re: mushboy]
    #23948685 - 12/22/16 10:20 AM (7 years, 1 month ago)

Bathrooms and kitchens are the two worst places to do mushroom work. But if you have a still air box you can work nearly anywhere in your house anyway


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Re: Grow Log: Worlds Worst Mycologist [Re: bodhisatta]
    #23948747 - 12/22/16 10:44 AM (7 years, 1 month ago)

:whathesaid: I bet I could use my SAB in a dumpster.


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #23949683 - 12/22/16 05:16 PM (7 years, 1 month ago)

Son of a.....



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OfflineKalsticky
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
    #23949965 - 12/22/16 07:35 PM (7 years, 1 month ago)

Quote:

Tiamo said:
Quote:

R34P3R said:
I would think that 10 days was plenty of time to see at least SOME growth




From spore to agar, rye or brf, no, it can take a lot longer than that. From a LC or LI, definitely.



Sounds like the OP just has some bunk syringes from a bad vendor. Ive had MS germinate on WBS in 3 to 4 days but usually for me MS takes about a week to germinate in room temp. Also you should never use more than 1 kind in each jar. Just use 1 type per jar and dont use too mush solution or youll make the water ratio in the jars off.


--------------------
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InvisiblemagicMerlin
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #23949969 - 12/22/16 07:37 PM (7 years, 1 month ago)

Quote:

R34P3R said:
Son of a.....






Not so bad

Clip the big pieces off, or better yet use a dremel if you have one.

Then you can use some low grit sandpaper and sand it down so it's more even at least.


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: magicMerlin]
    #23950043 - 12/22/16 08:03 PM (7 years, 1 month ago)

Quote:

magicMerlin said:
Not so bad

Clip the big pieces off, or better yet use a dremel if you have one.

Then you can use some low grit sandpaper and sand it down so it's more even at least.




My concern was more with the severe cracking. I am going to grab another box tomorrow and a hole bit. Thank you for the advice on how to salvage the bin though :smile:


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InvisiblemagicMerlin
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #23950537 - 12/22/16 11:29 PM (7 years, 1 month ago)

Ahh it's kind of hard for me to see it, but if it hasn't entirely split open the tub you could probably get away with taping it or silicone it, although it would probably hinder visibility. If a new tub is no big deal then I would do that :wink:

The best way I have found to make my holes is heat up a big empty can of beans
on your stove top for a minute and then press it on the plastic tote and slowly spin it. It makes a perfect circle for me every time. People get away with using hole saws too, but I find the tote is more prone to crack when using them. Make SURE to use an oven mitt and ventilate where you are melting plastic.

You sound like you've done a lot of research, but just a reminder, make sure you get the biggest SAB you can. If you plan to G2G gran jars in the future you should consider the height of the box as well. Check out this giant one.


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: magicMerlin]
    #23951378 - 12/23/16 11:13 AM (7 years, 1 month ago)

Quote:

magicMerlin said:

The best way I have found to make my holes is heat up a big empty can of beans
on your stove top for a minute and then press it on the plastic tote and slowly spin it. It makes a perfect circle for me every time. People get away with using hole saws too, but I find the tote is more prone to crack when using them. Make SURE to use an oven mitt and ventilate where you are melting plastic






Dremel to the rescue! Also I went with a 30gal tote. It was the tallest clear bin I could find 13.5"


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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #23951391 - 12/23/16 11:18 AM (7 years, 1 month ago)

Looks good


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
    #23952398 - 12/23/16 05:55 PM (7 years, 1 month ago)

Quote:

Tiamo said:
Looks good




Thank you. I had a chance to finish it up this evening.




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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #23995802 - 01/09/17 05:01 PM (7 years, 21 days ago)

I thought I would update this. I started using agar and now I have spawn for days. I have 2 mono and 1 shotgun going.  I also have 5 cakes ready to go with 3 more Pink Oyster, Golden Oyster and Lions main colonizing now. Thanks for all the advice.  Between the agar and the still air box , my beginner blues are over!


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: magicMerlin]
    #24026242 - 01/20/17 04:59 PM (7 years, 10 days ago)

The monos look like they will pin any day and the Pink cake is coming along :smile:


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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #24026293 - 01/20/17 05:11 PM (7 years, 10 days ago)

Take the gloves off it and itll be great.


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OfflineR34P3R
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Re: Grow Log: Worlds Worst Mycologist [Re: mrmazdarx9]
    #24026302 - 01/20/17 05:15 PM (7 years, 10 days ago)

Quote:

mrmazdarx9 said:
Take the gloves off it and itll be great.



Yes indeed I removed them after 2 uses. I also shortened the tubes sleeves. Thank you for the advice though:mushroom2:


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Registered: 05/15/16
Posts: 9,796
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
    #24026308 - 01/20/17 05:16 PM (7 years, 10 days ago)

:thumbup:


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COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN


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