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R34P3R
Stranger


Registered: 12/21/16
Posts: 21
Last seen: 3 years, 5 months
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Grow Log: Worlds Worst Mycologist
#23947767 - 12/21/16 11:51 PM (7 years, 1 month ago) |
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So after a discussion and a tiny bit of research I decided to grow some mushrooms. I mean it's fungi how hard could it be?
I consulted many YouTube videos and posts here. It became very apparent everything I need to know is found in the Marc Keith videos. I don't know that he invented the techniques but that is the point of origin on the net as far as I can tell. With that said, I opted to go with rye berries and skip the cakes. I watched the videos so I know this is going to be super simple!
Attempt 1:
Grocery List: Box of QT Jars Pressure cooker Alcohol and containers Organic Rye Berries Gypsum
I get all ingredients together Washed rye Soaked rye with gypsum for 24 hours Brought to boil for 10 mins Strained Let steam dry with occasional mixing until it passed the tissue test Loaded jars Put small hole in the top and filled with polyfill Covered with foil PC 15psi for 90 mins Let sit overnight to cool
I used a small bathroom to inoculate that I cleaned thoroughly I then ran an air purifier for about 1 hour I then sprayed the air with Lysol and allowed it to settle I inserted the needle through the polyfill I sprayed 1cc in each jar
I placed the jars on a shelf at room temperature
I did not touch them for a week and then took them down to see how things were going. To my surprise... they weren't! Nothing had happened. No colonization of anything. I waited another week and still nothing. Bird food they became.
Attempt 2:
Grocery List: Micropore Tape Sunflower Lecinthin Coir Pickling Lime
I repeated attempt one with the exception of the following:
I washed the rye after the soak overnight and used fresh water to boil in. I added sunflower lecithin to the boiling berries I boiled for 20mins instead of 10 I used micropore tape instead of polyfill I fire sterilized between jars when I inoculated
I had given thought to what substrate and casing method to use. I opted to go with coir so I picked that stuff up as well.
I waited 10 days before checking on these jars. I pulled them down in anticipation and... nothing. Not a damn thing. WTF is going on!
I reviewed all the steps and tried to find error or suggestions others had for nothing grows. The colonizing process seems very simple. There are only a few variables that are going to cause issues; contamination was not an issue since nothing at all was growing, substrate did not seem like it was the issue since I verified no fungicide was used in the product, bad spores which came from a known vendor so I did not immediately think that and finally water. I was not concerned about the amount of water but more the quality. We have well water which gets treated with chlorine and salt. I thought that had to be it!
Attempt 3: Grocery List: Distilled water...Lots of Distilled water
I repeated all steps in attempt 2 with the exception of using distilled water. I was also on syringe 2 at this point.
I once again waited 10 days and AGAIN nothing. Something is terribly wrong. I went back over my Tek and could not find any hard reasons why this is happening. I read more threads on no and stalled growth here. One thing that some people talked about was air exchange so I thought what the hell and took the lid off one of the jars. A few days later BOOM WE HAVE GROWTH but it was mold at least I now knew my jar was capable of supporting life. I came to the conclusion it had to be the spores.
Attempt 4: Grocery List: New spore syringes
I repeated the steps used in attempt 3 except I went back to tap water. After further research it appears the chlorine will be removed in the heating process.
Again nothing. I thought maybe it was not warm enough so I put a heat bad indirectly by the jars to heat them more. And boom we have growth ... shit... mold. Ok I am just going all out on the next one and trying every syringe.
Attempt 5 Grocery List: Freebie!
I repeated attempt 4. The exception is that I used 2cc sprayed to the edge of the jar and not the center with 1 strain per jar. I did not use the heat pad again. More post reading on here I concluded room temp is just fine (75f here)
Again nothing. This is when I gave up on rye and pretty much decided I could not grow mushrooms. Time went by and it really bugged me that I could not get this to work. I watched all the videos again. I read many more posts here and decided to have another go. I decided to go with the noob proof cakes this time. I also opted, for some unknown reason to grow the spores on agar as well.
Attempt 6 Grocery List: Wide mouth small jars Verm Perlite Brown Rice Flower Scale Beakers MEA Petri Dishes
I followed the BF tek from the let's grow mushrooms series exactly to the letter. For the agar I mixed just shy of 5g MEA to 200ml of water. I loaded everything up together and off we went and then I noticed the silence. The PC is not making any noise! Yea the worst has happened and I boiled it dry causing a huge bow in the bottom of my PC. I figure the agar was still ok to use. So I salvaged that an poured dishes. I followed the same procedure as inculating jars but using only a couple drops of each strain per dish. I did make sure there were a little bit of visible spore clumps since the syringes were dense. After a week no mycelium but plenty of bacteria and some mold on 2. I assume this was due to the PC failure but I am going to use distilled water next time.
Attempt 7 Grocery List; Pressure Cooker (I went with the big boy All American this time) Distilled water Glass Petri dishes
This attempt was identical to 6 with exception of distilled water in place of tap for making the agar. I inoculated the cakes using the described method in the video with exception of no glove box. I waited about 5 days and checked the cakes and the dishes. No growth in either. Good news is no contamination on the agar this time. I contacted the spore vendor and explained the situation. They agreed this is an odd issue but just to be sure they are replacing my syringes! That is some outstanding customer service.
I am thinking this is not going to be my thing.... but wait there is more... So today I am cleaning up my office that had turned into a lab. Tossing the Petri dishes. I grab the jars to go clean out and I look down at the first jar I pulled out and Boom we have growth! I looked closely and yes it is nice dense white and right at the inoculation points. I check the other jars and YES all but one is growing!
So that is the grow log to this point and I am really getting hooked on this stuff Any other ideas on the rye issue would be greatly appreciated.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R] 1
#23947792 - 12/22/16 12:04 AM (7 years, 1 month ago) |
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Patience, Try to leave them on the shelf until a shake is required(30%). Agar work should come before grain work. MS > grain is sometimes a let down.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 21 days
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Re: Grow Log: Worlds Worst Mycologist [Re: wtfcrazymofo]
#23948097 - 12/22/16 04:09 AM (7 years, 1 month ago) |
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Yeah it can take 2 weeks to germinate spores - you just need to play the waiting game
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 6 hours, 27 minutes
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Re: Grow Log: Worlds Worst Mycologist [Re: Mattisfat]
#23948115 - 12/22/16 04:38 AM (7 years, 1 month ago) |
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Sounds to me like you didn't wait long enough. You can wait up to 2/3 weeks for spores to germinate on agar or for any growth to appear in the rye or brf cakes. The colonising part is very slow.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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R34P3R
Stranger


Registered: 12/21/16
Posts: 21
Last seen: 3 years, 5 months
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
#23948179 - 12/22/16 06:28 AM (7 years, 1 month ago) |
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Thanks for the responses. I admit that the last 5 day wait was being quick on the gun, but I would think that 10 days was plenty of time to see at least SOME growth No? Perhaps my expectations were a bit optimistic. I plan to try the Rye again when I can inoculate from the petri. Who knows how long that will take me to figure out lol Hopefully the rest of the cake process will go smooth.
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
#23948210 - 12/22/16 06:58 AM (7 years, 1 month ago) |
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Quote:
R34P3R said: I would think that 10 days was plenty of time to see at least SOME growth
From spore to agar, rye or brf, no, it can take a lot longer than that. From a LC or LI, definitely.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
#23948271 - 12/22/16 07:48 AM (7 years, 1 month ago) |
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Stay away from water softeners!! They fucked with my grows something fierce. Also dont use distilled water.
Also a bathroom is a terrible place to inject jars.
Use the spiltball link in my signature. All you need to know is within.
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R34P3R
Stranger


Registered: 12/21/16
Posts: 21
Last seen: 3 years, 5 months
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Re: Grow Log: Worlds Worst Mycologist [Re: mushboy]
#23948292 - 12/22/16 08:07 AM (7 years, 1 month ago) |
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Quote:
mushboy said: Also a bathroom is a terrible place to inject jars.
From what I have seen, my bathroom is cleaner then where 95% of people are inoculating I kid. I have a spare bathroom that is only used by guests but still gets cleaned twice a week. All surfaces are solid and easy to sanitize. It is also the smallest suitable room. I understand the word bathroom does not sound sanitize'y but I assure you it was. My issue was not contamination, it was lack of any growth at all. I do think the water is playing into it somehow but I am not sure how yet. If I continue on I will make a glove box and if I go further I will graduate to the hood... Baby steps 
Thank you for the link I will have a look!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R] 1
#23948379 - 12/22/16 08:45 AM (7 years, 1 month ago) |
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glove box.. still air box!
They take less than 5 minutes to make my friend. If your bathroom is still with the vents closed, having a still air box inside of a room that's still just adds onto your success rates.
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 6 hours, 27 minutes
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
#23948390 - 12/22/16 08:50 AM (7 years, 1 month ago) |
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My glovebox cost me €10 for a 130 L clear tote, I drilled with a hole saw and was done in <2 mins. You can use a coffee can heated on a stove or a sharp knife too.
Would recommend, but don't think that's your issue. Your issue is just patience. I definitely recommend going agar first and inoculating with a blenderless LI, you will notice growth much faster. Make sure you have a clean, full plate of mycelium.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
#23948392 - 12/22/16 08:52 AM (7 years, 1 month ago) |
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Fuck the sharp knife/screwdriver method. Use scissors instead.
https://www.shroomery.org/forums/showflat.php/Number/23897456
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 6 hours, 27 minutes
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
#23948399 - 12/22/16 08:54 AM (7 years, 1 month ago) |
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I have a real sharp Spyderco knife, real nice blade and well sharpened, see my gallery. It will slice your scizzors right in half.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
#23948408 - 12/22/16 08:58 AM (7 years, 1 month ago) |
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What's funny AF is those scissors I used were already broken in half. They had no handles, as you can see by my weird way of holding them. It's so fuckin easy to cut a box up with them. Just like cutting a nice circle in paper. I really couldn't imagine doing that with a knife all that easily. Maybe you could prove me wrong on that 
And for germinating lame spores agar is the best route. Charcoal agar, sandwich teks, hot pour, etc are stuff people do to germinate shit spores
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 6 hours, 27 minutes
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
#23948417 - 12/22/16 09:02 AM (7 years, 1 month ago) |
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I have a feeling the serrated edge + heat will feel slice through it like butter, similar to scissors but I have no need for a second SAB. So not in a rush to prove it to you. Anyway, getting a bit off-topic. I'll stray away.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
#23948421 - 12/22/16 09:05 AM (7 years, 1 month ago) |
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I don't even have a knife like that to try xD. Broke ass over here. I do have a blunt katana tho
And yeah I really don't think the lack of SAB is the problem with tricky spores. But agar is the shit for tricky spores IMO. There's only ever been 1 syringe I've never been able to get to germinate on agar and the various forced germination methods
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Grow Log: Worlds Worst Mycologist [Re: Mad Season]
#23948540 - 12/22/16 09:47 AM (7 years, 1 month ago) |
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dont use a knife. your SAB will look like mine. it works but yikes...

BACK at OP..
i didnt say your failed because of contams. i said a bathroom is a bad place to work. they have built in mold growers in the floors(toilets).
water is playing into it because softeners have salt added to the water. salt is bad for mushrooms. i think distilled also has salt. you want a water rich in minerals. not salt.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Grow Log: Worlds Worst Mycologist [Re: mushboy]
#23948685 - 12/22/16 10:20 AM (7 years, 1 month ago) |
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Bathrooms and kitchens are the two worst places to do mushroom work. But if you have a still air box you can work nearly anywhere in your house anyway
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Grow Log: Worlds Worst Mycologist [Re: bodhisatta]
#23948747 - 12/22/16 10:44 AM (7 years, 1 month ago) |
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I bet I could use my SAB in a dumpster.
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R34P3R
Stranger


Registered: 12/21/16
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Re: Grow Log: Worlds Worst Mycologist [Re: R34P3R]
#23949683 - 12/22/16 05:16 PM (7 years, 1 month ago) |
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Son of a.....
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Kalsticky
royale with cheese


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Re: Grow Log: Worlds Worst Mycologist [Re: Tiamo]
#23949965 - 12/22/16 07:35 PM (7 years, 1 month ago) |
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Quote:
Tiamo said:
Quote:
R34P3R said: I would think that 10 days was plenty of time to see at least SOME growth
From spore to agar, rye or brf, no, it can take a lot longer than that. From a LC or LI, definitely.
Sounds like the OP just has some bunk syringes from a bad vendor. Ive had MS germinate on WBS in 3 to 4 days but usually for me MS takes about a week to germinate in room temp. Also you should never use more than 1 kind in each jar. Just use 1 type per jar and dont use too mush solution or youll make the water ratio in the jars off.
-------------------- "Nature loves courage. You make the commitment and nature will respond to that commitment by removing impossible obstacles. Dream the impossible dream and the world will not grind you under, it will lift you up. This is the trick. This is what all these teachers and philosophers who really counted, who really touched the alchemical gold, this is what they understood. This is the shamanic dance in the waterfall. This is how magic is done. By hurling yourself into the abyss and discovering its a feather bed.” ― Terence McKenna
T.C. Mexicube. P.C. Brazilian
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