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Offlinetealseal
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well im back and in it for the long haul
    #23942337 - 12/20/16 02:49 AM (7 years, 1 month ago)

well im only just getting started really....
i have had a few grows under my belt, some maza, AA, golden teach,

started on brf, mehhhh

moved to bulk tubs, did quiet decent for a newb pulled an ounce here pulled another there, have yet to get a really beautiful pinset, still chasing the dream lol..

played around with agar a bit that was fun (not really, hadnt seen the green mold before that, wowwza)

basically dipped my toes in just about everything,

played the dicey game of going from spores to lc hahahah fun stuff, oh this looks good let knock up some WBS, never smelled a fouler smell in my life lmaoooo

so i did some reading lots of research and im back

as we speak... or as i type im PC'in some pastey plates cause i wanna really get the full effect out of what agar has to offer. i followed his TEK to a T, only i used some green food coloring and some MEA and  tsp of karo,

really basic shit, thanks for making it easy for us PASTEY!!! not sure if thats ur name but i just worked an 8 hr shift and im spent, really fighting to stay awake but only 20 mins left lol

i was blown away when i seen this tek it was right under my nose and i didnt even see it, after wasting money and time on petris
( i ordered the ones with 3 spots like in RR's videos, shit took 3 months shit came from hong kong like WTFFFF)

lmao anyways im here once more to make some friends and show off my skills n grows to benefit the next guy...


OK SO MY PLAN!!!!

i have some really lovely prints i took a while back
(couple months ago)

gonna hit a few with some more maza, AA, and GT's

and i have a few spore syringes id like to use, some equador, creeper and pink buffalo's, we shall see....

the fun begins tomorrow morning

stay tuned:)


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OfflineTh3Issu3
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Re: well im back and in it for the long haul [Re: tealseal]
    #23942453 - 12/20/16 05:50 AM (7 years, 1 month ago)

Good luck man! Here's a couple of things I wrote the otherday.

Quote:

Th3Issu3 said:
I love seeing this everyday.


To be honest, I feel like it all boils down to how far you want to take this hobby.


I went years going ms syringe>grain. I also went years without a flow hood. In that time I had some awesome grows, full canopy's and tub after tub. I did tons of jars and bags and worked my ass off in my SAB. I had contams, everyone does. It was like shooting dice. I just bought more grain and more syringes and trucked forward.

Over last summer I took a break, I suggest everyone who's ever been a slave to there PC to take one a year. This year when I came back at it, I told myself I was going to take it to the next level.

I bought a flowhood. Told myself I was going to get into this agar hype all the older guys and Tc's always swore by. So that's what I did. Got some already mixed potato dextrose agar. The simplest kind I could find. Just add water.

..Now at this time I was so used to blowing through 5-10 syringes a month and having so many contam issues I went out and bought 10 gt syringes and 10 Bplus.


It's been three months after I started working with agar and with my flowhood. I have 9 of each of the needles left. Have had basically no contams and im rocking and rolling at a 100 % contam free rate on all my jars and tubs. I'm saving money on syringes b/c its only taken one drop to make a plate which i clean up then make 4 more plates, then tiger drop into masters then grain to grain. Saving tons of money on grain, and there is less clean up.

Basically for me, It's now a no brainer. Can you go from ms syringe to grain.... Sure, why the fuck not. Will you save time, money, and heartace going to agar first? fuck yeah dude!

It's all boils down to how far you want to take this hobby my friend.

Good luck




Quote:

Th3Issu3 said:
When working with agar, my goal is clean healthy growth. I'm not trying to isolate anything. So the amount of transfers I do solely depends on that. Each time I transfer from my M.S. plate I also label it "T" then the number, for example; T1,T2,T3,ect... Usually 'T-2' Is where I find myself before cutting the plate up and using it for inoculation.

My process in this, is in steps like this......

MS to petri, labeled 'ms p'
'ms p' to 3 new plates labeled 'T 1'
3, 'T 1' plates to 3, 'T 2 ' plates

*all plates are saved for a week or so in case i need to use them again. But after clean growth on the donor plat is achieved I toss them.

Now depending how the plates look 't-2' is where I start using wedges. Not always... But like i said, depending if they are clean on not. 

So I now have 3, 't-2' petri's with clean growth. 

I make 16; 1 pint master.

I now take plate 1 and 2 labeled "T-2" and cut it up and use them to innoc the 16 1 pint grain masters. I do this b/c I take the 1 pint grain master after 100 % colonization to inoculate 2, 2 quart grain bags. In which I just use half of the jar in each bag. That's what I use for a tub. About 4 quarts of spawn...

OK now you have one plate left...

First thing i do is make 3 slants. I do this so i dont have to start from scratch...

So I take a small pieces from my t2 agar plate and drop them in the slant and label them T-3' then the strain and date. Those get set aside to fully colonize. Once they are, they get put in a lunch box in the fridge. ( I will will use this in the up coming months)

You still have the 't-2' pate just a few chunks missing...So what do we do...More transfers... Now following what I do here you can keep going down the line as far as you want.

I take the "T-2" petri and make 3 plates. Labeled "T-3"...Then I just do the same thing I did previously. Take two plates for masters then use one to go further down the line.

I try not to go farther than 't-6'. I'm not trying to isolate genetics. Just want clean growth.

When you transfer out of t-6 bring your slant out and do the same thing, work up to t-6.

IDK how others do it, but this has worked well for me. Wish you the best!!





Hope its of some use to you


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InvisibleMateja
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Re: well im back and in it for the long haul [Re: tealseal]
    #23942456 - 12/20/16 05:51 AM (7 years, 1 month ago)

Good Luck! :smile:

I recently spawned my first Mini and Mono after a few dozen BRF cakes.
In 1.5 months i'm moving to a new place and i will have my own work room
for the first time.

I'm already on the lookout for materials for EVERYTHING! I'm gonna have
GH, Minis, Monos, SGFC's and everything in between.

In other words, i will experiments as much as i can
while i'm reading books and lurking Shroomery!

My personality is perfect for this because i sort of get obsessed with
learning EVERYTHING about a subject when i find it interesting :laugh:


--------------------
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Offlinetealseal
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Registered: 01/19/16
Posts: 678
Last seen: 6 months, 20 days
Re: well im back and in it for the long haul [Re: Mateja]
    #23945993 - 12/21/16 12:31 PM (7 years, 1 month ago)

ya i lurked about for a while before trying my hand at it, it really is enjoyable to me. i love the feeling of a fresh mushie, so soft and delicate.

i too went a little crazy after my first brf cakes, got a whole 20 gallon tote full of supplies, from liners, a ton of jars, brf, and all that comes with.

i will say i spent a little too much on some things that were a bit advanced for my level, i got a whole roll of i forget what its called atm just waking up but u wrap ur petris and slants with it, not really necessary at this point because im playing with pastey plates.

and ive spent hundreds on pre made sub, im going to start making my own cause i dnt like having stuff like that shipped. but i just really love their sub, mushrooms pluck with ease, not too many holes n such afterwards..


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: Th3Issu3]
    #23946066 - 12/21/16 12:54 PM (7 years, 1 month ago)

Quote:

Th3Issu3 said:
Good luck man! Here's a couple of things I wrote the otherday.

Quote:

Th3Issu3 said:
I love seeing this everyday.


To be honest, I feel like it all boils down to how far you want to take this hobby.


I went years going ms syringe>grain. I also went years without a flow hood. In that time I had some awesome grows, full canopy's and tub after tub. I did tons of jars and bags and worked my ass off in my SAB. I had contams, everyone does. It was like shooting dice. I just bought more grain and more syringes and trucked forward.

Over last summer I took a break, I suggest everyone who's ever been a slave to there PC to take one a year. This year when I came back at it, I told myself I was going to take it to the next level.

I bought a flowhood. Told myself I was going to get into this agar hype all the older guys and Tc's always swore by. So that's what I did. Got some already mixed potato dextrose agar. The simplest kind I could find. Just add water.

..Now at this time I was so used to blowing through 5-10 syringes a month and having so many contam issues I went out and bought 10 gt syringes and 10 Bplus.


It's been three months after I started working with agar and with my flowhood. I have 9 of each of the needles left. Have had basically no contams and im rocking and rolling at a 100 % contam free rate on all my jars and tubs. I'm saving money on syringes b/c its only taken one drop to make a plate which i clean up then make 4 more plates, then tiger drop into masters then grain to grain. Saving tons of money on grain, and there is less clean up.

Basically for me, It's now a no brainer. Can you go from ms syringe to grain.... Sure, why the fuck not. Will you save time, money, and heartace going to agar first? fuck yeah dude!

It's all boils down to how far you want to take this hobby my friend.

Good luck




Quote:

Th3Issu3 said:
When working with agar, my goal is clean healthy growth. I'm not trying to isolate anything. So the amount of transfers I do solely depends on that. Each time I transfer from my M.S. plate I also label it "T" then the number, for example; T1,T2,T3,ect... Usually 'T-2' Is where I find myself before cutting the plate up and using it for inoculation.

My process in this, is in steps like this......

MS to petri, labeled 'ms p'
'ms p' to 3 new plates labeled 'T 1'
3, 'T 1' plates to 3, 'T 2 ' plates

*all plates are saved for a week or so in case i need to use them again. But after clean growth on the donor plat is achieved I toss them.

Now depending how the plates look 't-2' is where I start using wedges. Not always... But like i said, depending if they are clean on not. 

So I now have 3, 't-2' petri's with clean growth. 

I make 16; 1 pint master.

I now take plate 1 and 2 labeled "T-2" and cut it up and use them to innoc the 16 1 pint grain masters. I do this b/c I take the 1 pint grain master after 100 % colonization to inoculate 2, 2 quart grain bags. In which I just use half of the jar in each bag. That's what I use for a tub. About 4 quarts of spawn...

OK now you have one plate left...

First thing i do is make 3 slants. I do this so i dont have to start from scratch...

So I take a small pieces from my t2 agar plate and drop them in the slant and label them T-3' then the strain and date. Those get set aside to fully colonize. Once they are, they get put in a lunch box in the fridge. ( I will will use this in the up coming months)

You still have the 't-2' pate just a few chunks missing...So what do we do...More transfers... Now following what I do here you can keep going down the line as far as you want.

I take the "T-2" petri and make 3 plates. Labeled "T-3"...Then I just do the same thing I did previously. Take two plates for masters then use one to go further down the line.

I try not to go farther than 't-6'. I'm not trying to isolate genetics. Just want clean growth.

When you transfer out of t-6 bring your slant out and do the same thing, work up to t-6.

IDK how others do it, but this has worked well for me. Wish you the best!!





Hope its of some use to you





damn man it took me a minute to understand wat ur saying but i get it now. i tried a few petris and agar work and failed miserably, that being said i wasnt going to let it get me down, i took a break read A LOT and now im back. with only a few grows under my belt and most have been tray grows, i feel as soon as i get a nice plate going ill do some agar to grain, then GTG, and have enough spawn to do a real, full mono.

i have no where to dump my bad spawn cause where im at i cant really be making patches outdoors, at first i tried to clean all the jars that went bad on me, being really cautious about it but i just dnt like the feeling of walking back into my house afterwards, so i take em out to my gmas and toss em in a hole, i have yet to see if anything has come of it but i got alot going on...

also i dnt know why maybe its because my landlords a ex cop but i dnt even like pouring my bad lc's down the sink or shooting wats left of any myc down my sink... not that i really know if anythings gonna happen but i dnt want to risk it..

ive wasted pounds of WBS on bad myc.
thats wat im trying to avoid so im going to work soley on getting clean growth on these plates, i dnt mind starting over if i have to do so.

i was tired of wasting and the constant cleaning of the jars i said, enough with this there has gotta be an easier agar tek and sure as shit i done found  skeeter.

i have yet to put up any pics, i got it all done yesterday, and with wanting to be as sterile as i could i just avoided taking em all together...


before i posted this thread, i had taken wat i assumed to be the last few jars of my good spawn and started 2 more tubs, i just but them in to fruiting conditions last night and its looking good, smelled really good both in the jars before i mixed and when i fan, i get a really yummy mushroom smell,
trust me ive smelled some BAD jars lol.

so with that being said ill get some pics up as we progress, im just trying to get this thread going in the mean time,

in the past ive asked stupid questions but i feel thats all behind me, i have a few im sure ill ask throughout this grow but not as many as a newb would, im familiar with the lingo and the simple things that can make a newb go wtf are they talking about!!!! lol



ok, so i went ahead and started some maza from a print i made,was going to do some albinos but i only started 6 plates for now,

my first try at the swab n roll method was ok, i didnt feel like spores even got in there so i did another plate of maza, idk if i did too much but i wanted to see black on the agar so i made sure i got some specks on there.

i went and hit another 2 with a syringe ive been wanting to try ever since i picked it up from one of the vendors here, i got some creeper, and hit 2 plates.

on the first plate of creeper, i hit it with 1 drop, the second plate i did 2 drops. (playing around with some stuff to find out what works best)

ok so 4 plates down only have 2 left wat did i do......

well i hit one with 1 drop of pink buffalo
(that name just sounds cool lol and the fruits from wat i seen are gorgeous)

the other i did 1 drop of equador

now time will tell, i have more of those glad tight seal thingy majigs so ill use those for transfers as i need too.

the tubs i have ready to fruit were the last good jars i had made from a master GT jar, in which i took a clone put it on the only petri dish that i successfully made, and let it grow out a bit its in one of my threads...

threw a piece in a jar then did a few GTG transfers, i ran the others in the past and got some fruits like this









man i wish i cloned that puppy look at her!!!

lol anyways that about wraps it up ill let u guys know how it goes..

and heres a lil porn of my past work, dnt beat me up too bad im still a newb lol

MAZA (one of my favorites fucking mind melters man)





ALBINO A+









thanks for looking:)


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: tealseal]
    #23961072 - 12/27/16 06:00 PM (7 years, 1 month ago)

well its been a week since i started some plates from spores and not seeing anything yet, also got some slants the other day of some TOC and PE and i did a few transfers and yet to see any growth on them either its only been 3 days on those guys...

meanwhile the tubs i started fruiting are yet to show me anything either.... this hasnt happened to me before.

the pasty plates are in a closet thats about 70f. and at night it drops to 68f

both the room my trays and my paltes are in are the same temps.... should they be higher?

is slow growth because of how cold it is??


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OfflineKenetic
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Re: well im back and in it for the long haul [Re: tealseal]
    #23961081 - 12/27/16 06:08 PM (7 years, 1 month ago)

If you are really at that temp then you have a different problem, like technique.

That hybrid mono though....how's that working out for you?  Not that great apparently?


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleIntelligentxfruit
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Re: well im back and in it for the long haul [Re: Kenetic]
    #23961117 - 12/27/16 06:29 PM (7 years, 1 month ago)

Thats why agar is great, even if you have a contam on your plate its easy as pie to isolate clean growth to the next plate. Cuts down contams by a huge amount.


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OfflineKenetic
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Re: well im back and in it for the long haul [Re: Intelligentxfruit]
    #23961124 - 12/27/16 06:34 PM (7 years, 1 month ago)

I just recently had an AA syringe that had at least 3 different molds plus bacteria, but using agar I turned it into this:











Not AA as it turned out but you get the point.


Cold weather can certainly cause slow growth op


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: Kenetic]
    #23961280 - 12/27/16 07:49 PM (7 years, 1 month ago)

Quote:

kenetic said:
If you are really at that temp then you have a different problem, like technique.

what does my technique have to do with low temps??

what i was asking is if the lower temps are causing growth to be slow..


That hybrid mono though....how's that working out for you?  Not that great apparently?




its worked out quiet well actually, ive done a few grows with success, now i havent fully dialed it in but i havent made enough sub to fill the bottom of my newer big tubs, only reason this tub is a hybrid is because i messed up countless tubs with trying different teks, im still trying to dial it in.

but this has worked in the past i dnt know why its not working now.


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OfflineKenetic
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Re: well im back and in it for the long haul [Re: tealseal]
    #23961298 - 12/27/16 08:02 PM (7 years, 1 month ago)

Quote:

tealseal said:
Quote:

kenetic said:
If you are really at that temp then you have a different problem, like technique.




what does my technique have to do with low temps??

what i was asking is if the lower temps are causing growth to be slow..


Quote:

Kenetic said:
That hybrid mono though....how's that working out for you?  Not that great apparently?




its worked out quiet well actually, ive done a few grows with success, now i havent fully dialed it in but i havent made enough sub to fill the bottom of my newer big tubs, only reason this tub is a hybrid is because i messed up countless tubs with trying different teks, im still trying to dial it in.

but this has worked in the past i dnt know why its not working now.




Fixed.


Quote:

tealsea said:

what does my technique have to do with low temps??

what i was asking is if the lower temps are causing growth to be slow..






It doesn't, that's why I said the problem is something else.  :shrug:

yes, lower temps do affect growth.


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: Kenetic]
    #23961530 - 12/27/16 10:52 PM (7 years, 1 month ago)

im going to have to wait until the plates are done and i see clean growth, take a step back and run mini monos....

im going to use wat i hope is a clean lc syringe i pulled a while back. but im doubting that its gonna be good first transfer i may have to do a few.


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OfflineTheblingbling
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Re: well im back and in it for the long haul [Re: tealseal]
    #23961556 - 12/27/16 11:09 PM (7 years, 1 month ago)

good luck


--------------------
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OfflineLobi
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Re: well im back and in it for the long haul [Re: Kenetic]
    #23961644 - 12/28/16 12:05 AM (7 years, 1 month ago)

Quote:

kenetic said:
I just recently had an AA syringe that had at least 3 different molds plus bacteria, but using agar I turned it into this:











Not AA as it turned out but you get the point.


Cold weather can certainly cause slow growth op




FUCK :whacker:


--------------------
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The fellowship of atoms,
of star dust in its endless flight, of suns and worlds,  of gods and men.
The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit.

:mushroom2:My High Quality Lo-Fi Beats  -:mushroom2:MushroomCultivation Compendium  -:mushroom2:- Doing Bulk w/ No PC -:mushroom2:

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Offlinetealseal
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Re: well im back and in it for the long haul [Re: Lobi]
    #23965269 - 12/29/16 11:16 AM (7 years, 1 month ago)

i finally have some growth on my plates:)

my PE and TOC from slant to new plates and my maza from print are taking off:)

ill post pics in a bit


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: tealseal]
    #23968119 - 12/30/16 01:00 PM (7 years, 1 month ago)

i also have pins on the 2 tubs that weren't really doing anything:) wooo hoooo!!!!


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: tealseal]
    #23968177 - 12/30/16 01:31 PM (7 years, 1 month ago)

ok so here is some shots of the pasty plates:)

the pink are a mix of TOC and PE








and this plate is the maza from spore print
( this tek is the shit, and i get to use all the prints ive saved over the months)



a shot of all the plates i have going atm



and the PINS:)






Now i also went and picked up some super sick ass tubs at my local hardware store for 8 bucks a pop, heres the cool part, ive decided to run mini monos so these are 30QT

but get this, this is the best part:) they have these seals that keep put moisture, but those same seals are going to lock in the moisture causing less evaporation through the top of the lid





moisture lock ROCKS!!

anyway before i start drilling any holes (cause ive ruined about 10)
first i started SGFC style.... mehhh

then i made big monos (never made enough sub to fill the bottom)

so ive just been running aluminum trays in a HYBRID tub

ive started fresh ditched the old tubs and the old ways, finally have some good growth to do my transfers n what not, ive looked at a lot of tub teks and just would like an opinion on a legit tek for these sweet tubs.

P.S. hows the growth look on those agar plates, im still new with agar


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: tealseal]
    #23970642 - 12/31/16 12:29 PM (7 years, 30 days ago)

ok so im not exactly sure when to transfer to another dish, i was once told u  want to transfer when u see a quarter size growth before it gets over grown, so is it time to take transfers from this dish?
its from a maza print i took, being MS my first thought its to take at least 2 or 3 wedges and transfer em to a new dish
or could i just tiger drop it into a jar????


the next one was a PE wedge from a slant i was sent, was told to do a few transfers to make sure its clean but it looks like in a day or so i could just tiger drop this guy as well as is


and an update on the pins, this is the same tub as pictured up above just a day later, my other tub just started pinning as well..





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InvisiblemushboyMDiscord
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Re: well im back and in it for the long haul [Re: tealseal]
    #23970735 - 12/31/16 01:09 PM (7 years, 30 days ago)

the green plate is good to tr.. you could wait a bit. a larger culture is easier to work with.
even if i took the print id never do print-agar-jar. i always xfer at least once.

let the red one grow more.

tubs look good bro.


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Offlinetealseal
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Re: well im back and in it for the long haul [Re: mushboy]
    #23970777 - 12/31/16 01:25 PM (7 years, 30 days ago)

Quote:

mushboy said:
the green plate is good to tr.. you could wait a bit. a larger culture is easier to work with.
even if i took the print id never do print-agar-jar. i always xfer at least once.

let the red one grow more.

tubs look good bro.





ya i planned on doing a few transfers before i went to a jar, but with the pink one the PE, when i was given it i was told to do a few transfers but it looks good already, so i will do a few more while i wait for these tubs to finish...

thanks man! took a while but after these hybrid tubs are done im going to do it right, idk wat i was thinkin tbh, but ever grow has worked in these tubs so its money well spent i guess, but im going starting from square 1,

thanks for the response bro i was going to give em till monday cause i got the day off and ill just mess with transfers n shit then:)


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