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OfflineAK1000
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What are the pros & cons of inoculating with LI versus G2G?
    #23939014 - 12/18/16 07:36 PM (7 years, 1 month ago)

I can make one batch of blenderless LI and inoculate many bottles with it. Or, I could get a quart of colonized grain, and inoculate a bunch of bottles with it.  Both are extremely easy to use.

So what are their differences?

For the LI
  • I need to wait for a clean culture of agar to be almost completely colonized.
  • I need to sterilize a jar of some water in the PC so it takes up one spot in my PC.

For the G2G, I need to wait for a jar of grain to colonize. That's all I could come up with.

Both of them provide a multitude of inoculation points when shaken up, so I'm not sure which one will be faster or have any other difference. Got any other ideas?


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-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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InvisibleIntelligentxfruit
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: AK1000]
    #23939030 - 12/18/16 07:41 PM (7 years, 1 month ago)

personal preference.

I think some try to claim LI as faster, but without solid tests its anecdotal.


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OfflineNDStepp84
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: Intelligentxfruit]
    #23939578 - 12/19/16 01:00 AM (7 years, 1 month ago)

If you are going to use that quart to g2g you better be waiting on some agar to inoculate it with as well.  So then you could drop the agar in that qt to be expanded g2g, or LI it to 10 quarts in that time but as said personal preference. Which ever works best for you and your situation is the best methodIMO, it's good to practice other methods and have more tools in your tool box so to speak and keeps the hobby fun and interesting.


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

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Offlineenlightenment
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: NDStepp84]
    #23939598 - 12/19/16 01:19 AM (7 years, 1 month ago)

Pro LI/LC:
  • Possible to inoculate without exposing the the receiving jar to air. (sterile inoculation)


IMO this is the biggest advantage.
I like both. G2G and using liquid cultures.
All of my lids have an injection port.


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OfflineDactylium
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: enlightenment]
    #23939646 - 12/19/16 02:18 AM (7 years, 1 month ago)

LI makes it easy to distribute agar between a bunch of jars. It also minimizes contact between surfaces so bacteria have a more difficult time hitching a ride into your spawn.


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Invisiblewildernessjunkie
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: Dactylium]
    #23939682 - 12/19/16 03:16 AM (7 years, 1 month ago)

I can't believe there's such a thing as Sterile Inoculation. There's Aseptic, and the "Best You Can Do". In amateur mycology, it's all you can really ask for and expect.

With that said....

If you are reasonably sure that your existing colonized grain is clean, you can do both. ANY form of clean mycelial culture can be transfered.

Agar is best, but if you have good clean grain spawn it will work just as well. Slurry it in the blender and you can use it the same as LI.


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OfflineKenetic
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: wildernessjunkie]
    #23939795 - 12/19/16 07:25 AM (7 years, 1 month ago)

I would much rather use li instead of g2g because grains often have bacteria in them, no matter what you do.  Better to inoculate with clean agar than bacterial grain.


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Todo Cambia
   

               
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OfflineMattisfat
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: Kenetic]
    #23939857 - 12/19/16 08:22 AM (7 years, 1 month ago)

G2g seems to be alot quicker for me but LI allows me to make 10 jars of grain straight from agar where as dropping agar to grain then doing g2g means that my first bulk amount of jars would be generation 2 if that makes sense.


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OfflineAK1000
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: Mattisfat] * 1
    #23939953 - 12/19/16 09:33 AM (7 years, 1 month ago)

Quote:

Mattisfat said:
G2g seems to be alot quicker for me but LI allows me to make 10 jars of grain straight from agar where as dropping agar to grain then doing g2g means that my first bulk amount of jars would be generation 2 if that makes sense.




Is it called generation 2, though? I thought it's just a continuation of the same genetic material without any isolation so it would be the same generation, no?


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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OfflineMattisfat
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: AK1000]
    #23939989 - 12/19/16 09:58 AM (7 years, 1 month ago)

Quote:

AK1000 said:
Quote:

Mattisfat said:
G2g seems to be alot quicker for me but LI allows me to make 10 jars of grain straight from agar where as dropping agar to grain then doing g2g means that my first bulk amount of jars would be generation 2 if that makes sense.




Is it called generation 2, though? I thought it's just a continuation of the same genetic material without any isolation so it would be the same generation, no?



It is a continuation of the same material yeah; i say generation 2 but im sure someone else could give it a better name - the myc expands alot in grain jars causing senescence over 2 or 3 g2g's which makes the culture grow weak and contam prone.


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OfflineMattisfat
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Re: What are the pros & cons of inoculating with LI versus G2G? [Re: Mattisfat]
    #23940171 - 12/19/16 11:49 AM (7 years, 1 month ago)

Also OP for LI the whole puck doesnt need to be colonised - just cut out the area you need just like agar to grain - obviously the more the better


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