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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Pasturized vs sterilized bulk subs.
#23938336 - 12/18/16 02:59 PM (7 years, 1 month ago) |
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Some seem to believe that with cubensis a sterile substrate will perform better..i am not one of them as over the years i have watched great growers go both routes and have done both myself for years so would like to hear your thoughts on this subject and encourage you to test it..i will be testing this myself with my pe6 culture.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
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Re: Pasturized vs sterilized bulk subs. [Re: cronicr]
#23938372 - 12/18/16 03:11 PM (7 years, 1 month ago) |
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Back when I was running my ksss it was all sterilized sub and they did great. Pasteurized sub works just as well ime
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pasturized vs sterilized bulk subs. [Re: cronicr]
#23938378 - 12/18/16 03:13 PM (7 years, 1 month ago) |
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That does work and in two ways. The longer you cook a substrate the more it breaks down. Friendly bacteria in a pasteurized sub need energy and nutrients, yield loss.
Example: Horse poo is nicely broken down, by the horse.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Pasturized vs sterilized bulk subs. [Re: Ferather]
#23938397 - 12/18/16 03:19 PM (7 years, 1 month ago) |
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That is a simple assumption of more nures means more yield
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pasturized vs sterilized bulk subs. [Re: cronicr]
#23938406 - 12/18/16 03:21 PM (7 years, 1 month ago) |
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I did a KO sub with added probiotics, yield loss was about 20% and it took months.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Pasturized vs sterilized bulk subs. [Re: Ferather]
#23938425 - 12/18/16 03:26 PM (7 years, 1 month ago) |
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I will be trying sterilized coco next run.
i am monitoring this thread
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Pasturized vs sterilized bulk subs. [Re: mushboy]
#23938440 - 12/18/16 03:28 PM (7 years, 1 month ago) |
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Ive done coir and i have done a lot of straw n never seen any diff
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pasturized vs sterilized bulk subs. [Re: cronicr]
#23938476 - 12/18/16 03:40 PM (7 years, 1 month ago) |
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You probably won't with an open air capable substrate media, in normal condition. The more you breakdown straw or wood the greater the chance of mold.
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TheShroomster01
It's my first time, dont laugh.



Registered: 10/30/16
Posts: 226
Loc: EU
Last seen: 5 years, 6 months
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Re: Pasturized vs sterilized bulk subs. [Re: Ferather]
#23938558 - 12/18/16 04:12 PM (7 years, 1 month ago) |
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Lurking along.
-------------------- I'm European, i can't help it. "To harvest a cannabis plant to make hash corresponds to slaughtering a cow to make milk. Rub it!"
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 10 hours, 54 minutes
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-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Pasturized vs sterilized bulk subs. [Re: wtfcrazymofo]
#23938667 - 12/18/16 04:55 PM (7 years, 1 month ago) |
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K gonna put this culture to a plate then to masters..i will weigh out equal amounts of grain and water and equal amounts of bulk sub n water which will be cow and horse manure.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Pasturized vs sterilized bulk subs. [Re: cronicr]
#23938798 - 12/18/16 05:50 PM (7 years, 1 month ago) |
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im interested in seeing these results.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Pasturized vs sterilized bulk subs. [Re: liloldme]
#23938845 - 12/18/16 06:15 PM (7 years, 1 month ago) |
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What about oven tek coir? Any experience? I tried it once and it definitely didn't hurt.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Pasturized vs sterilized bulk subs. [Re: Kenetic]
#23938849 - 12/18/16 06:19 PM (7 years, 1 month ago) |
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Coir i wont even consider in this..you can do anything to that stuff.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasturized vs sterilized bulk subs. [Re: cronicr]
#23938909 - 12/18/16 06:41 PM (7 years, 1 month ago) |
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The only benefit I see in sterillising your sub is you can add things like dog food, grain water,bran or BRF that IME increase yeilds. If it's just a matter of sterilised manure VS pasteurized manure, I see no real difference.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Pasturized vs sterilized bulk subs. [Re: MudaFuka]
#23939004 - 12/18/16 07:33 PM (7 years, 1 month ago) |
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I guess its more full proof. Ive fucked up pasteurizing before, but never a PC cycle
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Pasturized vs sterilized bulk subs. [Re: mushboy]
#23939006 - 12/18/16 07:34 PM (7 years, 1 month ago) |
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Ive lost a lot of sterile grows with bags..shits tricky in a sab
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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Re: Pasturized vs sterilized bulk subs. [Re: MudaFuka]
#23939013 - 12/18/16 07:36 PM (7 years, 1 month ago) |
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Bacteria don't just use nutes, they also release them. its a whole cycle.
Honestly for this to be tested out there needs to be different levels of heat treated sub. Because really what you're looking at is the bio-availability of the substrate for the mycelium through the process of heat degradation.
So If i were to do this.. I would have a minimum of 3 groups
Pasteurized, sterilized, then cooked to shit beyond sterilized.
There instead of just an a & b group of which is better, you have third group to test the actual principle behind what we believe gives one an advantage over the other.
either way
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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plus if im not mistaken I read some commercial facilities used sterilized sub and then lab inoculate/colonize.
pretty sure I saw that in a video somewhere but its a reach in my memory.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Other species you cant really compare though..i am talking cubes in general.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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Re: Pasturized vs sterilized bulk subs. [Re: cronicr]
#23939039 - 12/18/16 07:44 PM (7 years, 1 month ago) |
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ah you did say cubes, yeah edibles throw out the commercial facilities lol.
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keifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
Last seen: 26 days, 8 hours
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You can't beat Damions coco tek.. Makes it so easy. Wish I know this and that coco gives comparable results to hpoo when I started
Edited by keifnnugs (12/18/16 07:58 PM)
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Pasturized vs sterilized bulk subs. [Re: keifnnugs]
#23939595 - 12/19/16 01:18 AM (7 years, 1 month ago) |
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I would presume that the results of both methods, when compared closely and accurately, would likely not have any measurable difference. This was my personal experience but my experience is just observations of random grows and random variables.
My stance on it would be; flip a coin. 
I'd love to see a real experiment with some good data. What a time to be alive.
-------------------- ¯\_(ツ)_/¯
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pasturized vs sterilized bulk subs. [Re: Tmethyl]
#23939845 - 12/19/16 08:04 AM (7 years, 1 month ago) |
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Bacteria vs no bacteria, an obvious answer is the time it takes to recover after a shake. Breakdown will improve speed only, in general. Not yield, potency or taste.
100g of straw vs 100g of straw flour will just be easier to digest.
Pretending that you could colonize a powder alone.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pasturized vs sterilized bulk subs. [Re: Ferather]
#23939849 - 12/19/16 08:13 AM (7 years, 1 month ago) |
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Here is bacillus cereus agar, as you can see it needs nutrients to grow.
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