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OfflineCrispykoot
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Culture slant tek
    #23937905 - 12/18/16 12:24 PM (7 years, 1 month ago)

Wondering what the opinions out there are for user friendly culture slant teks? :mushroom2:


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InvisibleJosex
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Re: Culture slant tek [Re: Crispykoot]
    #23937925 - 12/18/16 12:32 PM (7 years, 1 month ago)



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OfflineTiamo
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Re: Culture slant tek [Re: Josex]
    #23937942 - 12/18/16 12:41 PM (7 years, 1 month ago)

Why is it important that the agar is slanted?


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Offlineenlightenment
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Re: Culture slant tek [Re: Tiamo]
    #23937948 - 12/18/16 12:42 PM (7 years, 1 month ago)

culture slants are used for long term storage of cultures.


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InvisibleJosex
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Re: Culture slant tek [Re: Tiamo]
    #23937952 - 12/18/16 12:45 PM (7 years, 1 month ago)

Because you can keep cultures for a very long time in the fridge this way and the small surface area and minimal GE of slants is perfect for that.


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InvisiblePastywhyteMDiscord
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Re: Culture slant tek [Re: Josex]
    #23937956 - 12/18/16 12:48 PM (7 years, 1 month ago)

The slanted agar provides a little more surface area for the culture to grow on. The more of a slant you get the more pulls you can make from the slant.


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Re: Culture slant tek [Re: Pastywhyte]
    #23937962 - 12/18/16 12:50 PM (7 years, 1 month ago)

Okay thank you. :smile:


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OfflineCrispykoot
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Re: Culture slant tek [Re: Josex]
    #23937999 - 12/18/16 01:06 PM (7 years, 1 month ago)



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Re: Culture slant tek [Re: Crispykoot]
    #23940279 - 12/19/16 12:56 PM (7 years, 1 month ago)

Ok...One more question about slants. Are people happy with 17x100mm or is it too small? I can get 25x100mm or 25x150mm in glass or it's 17x100mm in a polypropylene tube with a ge/airtight cap. Thanks again...


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Offlineenlightenment
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Re: Culture slant tek [Re: Crispykoot]
    #23940315 - 12/19/16 01:11 PM (7 years, 1 month ago)

I use 20x75mm slants.
17x100 is a little tighter but it should work well. I have enough space to inoculate.



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Re: Culture slant tek [Re: Crispykoot]
    #23940324 - 12/19/16 01:15 PM (7 years, 1 month ago)

I made a thread a month ago asking for a good source for test tubes and I bought what this member below recommended. I haven't used them yet, but they look awesome for the job, very big and high quality. If you're from the US all the better, because the seller is based there.
Quote:

NumeroEno said:
You can use eBay, but Discount Vials sells them for cheaper and you can get them with autoclavable seals. They sell on eBay as well but it's cheaper to order directly off their site. Get the biggest ones they sell. They're pretty much the largest borosilicate test tubes available anywhere. 



Quote:

NumeroEno said:
Here are the ones you want. Make sure to select the autoclavable gasket option.

http://www.discountvials.com/20-x-150-mm-test-tube-threaded-w-screw-cap-pkg-of-20/

Edit: I just realized that you're in Europe. You can do a search and filter it by location. Sorry about that. 




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OfflineCrispykoot
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Re: Culture slant tek [Re: Josex]
    #23940719 - 12/19/16 04:07 PM (7 years, 1 month ago)

Thanks again.


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