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ConAcide
Stranger

Registered: 11/25/16
Posts: 2
Last seen: 7 years, 1 month
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How many cc's of MS per pound of rye berries?
#23935427 - 12/17/16 01:24 PM (7 years, 1 month ago) |
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Utilized search and was unable to find much.
I've been doing PF tek for a while with some great success, finally decided to upgrade a bit to grain bags as I can't afford a PC. I have 1x 1 lb bag of Rye Berries and 2x 2 lb bags of Rye Berries. Plan on knocking up the bags and the fruiting in a mono after colonization of the bags. About how many cc's of MS per bag? And do I need to pop the full cc amount in one spot, or move the syringe around a bit?
Thanks guys.
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 8 hours
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Re: How many cc's of MS per pound of rye berries? [Re: ConAcide] 1
#23935430 - 12/17/16 01:26 PM (7 years, 1 month ago) |
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Get a pc or stick to cakes. If you do agar all you need is .25 ml for infinite inoculate.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: How many cc's of MS per pound of rye berries? [Re: ConAcide] 1
#23935444 - 12/17/16 01:31 PM (7 years, 1 month ago) |
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Quote:
ConAcide said: Utilized search and was unable to find much.
I've been doing PF tek for a while with some great success, finally decided to upgrade a bit to grain bags as I can't afford a PC. I have 1x 1 lb bag of Rye Berries and 2x 2 lb bags of Rye Berries. Plan on knocking up the bags and the fruiting in a mono after colonization of the bags. About how many cc's of MS per bag? And do I need to pop the full cc amount in one spot, or move the syringe around a bit?
Thanks guys.
U need a PC.............
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ConAcide
Stranger

Registered: 11/25/16
Posts: 2
Last seen: 7 years, 1 month
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Re: How many cc's of MS per pound of rye berries? [Re: Edmunter]
#23935456 - 12/17/16 01:34 PM (7 years, 1 month ago) |
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Reckon that I should have said this, but the berries are pre-sterilized. I'm fairly certain I don't need a PC for that.
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 4 days
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Re: How many cc's of MS per pound of rye berries? [Re: The Mycologist]
#23935460 - 12/17/16 01:35 PM (7 years, 1 month ago) |
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1/2 CC per pound is good. One spot is fine.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: How many cc's of MS per pound of rye berries? [Re: ConAcide]
#23935492 - 12/17/16 01:46 PM (7 years, 1 month ago) |
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Quote:
ConAcide said: Reckon that I should have said this, but the berries are pre-sterilized. I'm fairly certain I don't need a PC for that.
Yep, thats slightly different. Use as little as u can. Any amount of solution will germinate. as without a PC its gunna cost u lots in substrate and other costs involved in not being able to expand your cultures.....
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 10 hours, 9 minutes
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Re: How many cc's of MS per pound of rye berries? [Re: ConAcide]
#23935516 - 12/17/16 01:55 PM (7 years, 1 month ago) |
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Edited by wtfcrazymofo (12/17/16 01:57 PM)
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