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Offlinekronikdaheghog
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issues with mycelium growth
    #23935327 - 12/17/16 12:40 PM (7 years, 1 month ago)

i have been using 1:1:2 (by volume) water:BRF:vermiculite in 1/2 pint jars, autoclaved, two holes in the top for inoculation, replaced foil, put in sterilite container with pad underneath for warmth in closet
temp/hygrometer reads: 75-79degrees:99%humidity
my problem is the cakes dont seem to want to 100% colonize - they only get to like 75% after 3 weeks (looks like normal white mycelium)...
Two questions about mycelium:
1) Am i doing something wrong? Need more holes in the top? If so, should I bother to do it now?
2) Can I try to fruit a non-fully colonized cake? Should I attempt now?


Fruiting chamber questions: wet paper towels, layer of sterilized perlite in sterilite container with heating source underneath
1) Does that sound appropriate for fruiting?
2) Best temp?
3) Do I need to put it in direct sun at all?

Thanks for the help


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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23935412 - 12/17/16 01:18 PM (7 years, 1 month ago)

Could be bacterial... Need to post a pic with a question to get a definitive answer.

Ditch the paper towels and heater... No need to sterilize perlite.

Search for how to build a proper "SGFC" - shotgun fruiting chamber.


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OfflineTira
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23935415 - 12/17/16 01:20 PM (7 years, 1 month ago)

Don't put your jars in a box, and you don't need to heat them unless your room temp is extremely low.

There are two explanations why your cakes won't colonize 100%:

1- Uncolonized areas are bacterial:  If this is the case, you can see sectoring (and probably some kind of little gap) between the colonized area and the uncolonized area.  Also, uncolonized area might seem darker or wetter than usual substrate.

2-Your cakes are suffocating because they are in a closed box.

You can fruit uncolonized cakes but you should clean the uncolonized parts first and give them a good wash with cold water.

You don't need to sterilize perlite, just wash it before filling it into your fruiting chamber.

Don't put mycelium in direct sunlight.  Ambient room light is enough.




Did you put filters on top of the inoculation holes?  Do you have a vermiculite layer on top of each jar?  How was your inoculation process like?  Where did you inoculate them?

Pictures could help us identify the problem better.



Btw you can find answers to all of your questions if you watch RogerRabbit's Let's Grow Mushrooms videos.


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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23935416 - 12/17/16 01:21 PM (7 years, 1 month ago)

4 holes in top per directions
Foil comes off stays off after sterilization, don't put new foil on
Heating pads are a big no
68-72 is good ie room temperature
Humidity shouldn't be 99% and during colonization humidity doesn't matter at all
Don't put holes in now they needed to be there from the start
You can try to fruit partially colonized cakes by Washing off the uncolonized portions

1b. No that sounds like a terrible fruiting chamber
And no not directly in sun. Use recommend fluorescent lighting. Links in signature to relevant information


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23935568 - 12/17/16 02:18 PM (7 years, 1 month ago)

thanks for the replies...obviously i dont know what the hell im doing, haha...
i didnt do the vermiculite layer on top of the jars...fail

i took pics of 4 sample jars...i think i have like 8 in all that look similar to this... the growth appears white, sometimes cobwebby, sometimes a little grayer
sorry the lighting is shitty...



i looked up shotgun terrarium...ill build one and go ahead with that...maybe just try to fruit all these and see what happens...

what is everyones opinion?


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OfflineTira
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23935652 - 12/17/16 02:54 PM (7 years, 1 month ago)

These jars have bacterial contamination.  A layer of dry verm on top of the jars serves as a layer of filter and protects the substrates from contamination.  I can't see the top of the lids but looking at those jars, I'm guessing there are no filters or anything on those holes right?

I'd keep the ones that are mostly colonized (the ones that come out of the jar as a block) and toss those weren't (the ones that fall apart).

Just watch this and the other parts of it, it'll take 20 minutes of your time and will save weeks of it.


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: Tira]
    #23967659 - 12/30/16 09:57 AM (7 years, 1 month ago)

what if i dont have medical tape for under the top of the jar? does that really matter that much?

thanks


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: Tira]
    #23967706 - 12/30/16 10:17 AM (7 years, 1 month ago)

should i always dunk and roll the cakes? ive never heard of doing that...
i can sterilize the verm beforehand, shouldnt be a problem


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OfflineTira
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23968482 - 12/30/16 03:39 PM (7 years, 1 month ago)

Quote:

kronikdaheghog said:
what if i dont have medical tape for under the top of the jar? does that really matter that much?

thanks




Well you need some kind of filter or what you're doing is basically inviting contamination by leaving the holes open for them.  Micropore tape over the holes, tyvek under the lid, polyfill stuffed into holes; any of these would be a barrier with airborne particles in your environment and the sterilized content of the jar.

Dry layer of verm also serves as a filter both works better when combined with some kind of filter.  I've done my first grow without filters on holes (but with vermiculite on top of the substrate) and did not have any issues except a little bit of  bacterial contamination.



Yes, you should always dunk and roll the cakes, mushrooms are %90 water and mycelium consumes most of the water in your jars during colonization, so you need to replenish that by dunking.

Having vermiculite on your cakes works both as a water reservoir for your cakes, and as a casing for your cakes (it helps them forming pins).


How did your jars do?  Did you get any fruits from them?


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: Tira]
    #23968513 - 12/30/16 03:51 PM (7 years, 1 month ago)

ill dunk em tonight, roll and place in fruiting chamber tomorrow. thanks for your help. will send pics


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OfflineTira
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23968534 - 12/30/16 03:59 PM (7 years, 1 month ago)

Quote:

kronikdaheghog said:
ill dunk em tonight, roll and place in fruiting chamber tomorrow. thanks for your help. will send pics




are we talking about the same jars you posted about 13 days ago?

If so, did they continue colonization?  How do they look?


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: Tira]
    #23970962 - 12/31/16 02:21 PM (7 years, 30 days ago)



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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23970971 - 12/31/16 02:23 PM (7 years, 30 days ago)

how does this look? they all were pure white, smelled strongly of mushrooms, floated in the water during dunk...i used sterile vermiculite for the roll
three inches of sterile (before water) perlite in the bottom of the SGFC

opinions?


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23970983 - 12/31/16 02:25 PM (7 years, 30 days ago)

id also like heat source opinions...its been around 60s where im at...house is not heated..
also, how much direct light? indirect light?

thanks


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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23970988 - 12/31/16 02:28 PM (7 years, 30 days ago)

You forgot the foil under the cakes. a 5000k to 6500k bulb directly above will work just fine.

Also 70-75 is the perfect temp range.


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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23970999 - 12/31/16 02:33 PM (7 years, 30 days ago)

colder temps will take a little longer but provide more robust fruits 60s will work but not ideal, can you turn the heat up in the room they're in? that's about the only way to go.


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: tryptkaloids]
    #23972731 - 01/01/17 11:13 AM (7 years, 29 days ago)

the humidity is fine (99% in the chamber) but im thinking about pouring water directly onto the cakes to speed up/produce larger fruit. i've read you can do that. is that true or is it a bad idea?

thanks for the advice on light and temp.


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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23972753 - 01/01/17 11:23 AM (7 years, 29 days ago)

Humidity should be more like 50-80 and only 90-99 within an inch of the surface of the cakes. If the whole chamber is 99 you wont have great results


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OfflineTira
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Re: issues with mycelium growth [Re: kronikdaheghog]
    #23973276 - 01/01/17 03:07 PM (7 years, 29 days ago)

Quote:

kronikdaheghog said:
im thinking about pouring water directly onto the cakes




Misting them is better than pouring water on them.  You can also do bottom watering along with misting.

I use mini cake foils like these:



Just fill a little amount at a time and only add more after they've sucked it up and the cup is empty or near empty


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Offlinekronikdaheghog
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Re: issues with mycelium growth [Re: Tira]
    #23976814 - 01/02/17 05:26 PM (7 years, 28 days ago)

Ok, that makes alot more sense, thanks Tira. I have been misting 2-3x/day. Ill invest in some mini cake foils.

No pins yet guys as far as I can see.

Im dunking one more tonight, will send a pic of the cake before dunking just to get your opinion.

Thanks again.


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