Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Spore germination on agar
    #23934952 - 12/17/16 10:09 AM (7 years, 1 month ago)

any tips for germinating spores from a print on agar?(PDA)
make it less/more nutritious? it seems to be taking forever.

after doing a noobly job of scraping a chunk of spores off foil and onto a plate
i searched around and realized i should of used a qtip and done the ol' z pattern.
i dont think ive read they need to be hydrated in anyway.

im only curious because the same print was made into a syringe and those are growing out now.
they were made 3days after i did straight spores to agar. :shrug:


Extras: Filter Print Post Top
Invisibler.lutece
gave Columbia her wings.
 User Gallery

Registered: 09/06/15
Posts: 745
Loc: ∅
Re: Spore germination on agar [Re: mushboy]
    #23934973 - 12/17/16 10:14 AM (7 years, 1 month ago)

How long ago was the plate noc'd up? I've read that it can take much longer for spores to germinate from a print. Up to two weeks, maybe three IIRC.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 8 hours
Re: Spore germination on agar [Re: r.lutece]
    #23934990 - 12/17/16 10:22 AM (7 years, 1 month ago)

:threadmonitor:


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Spore germination on agar [Re: r.lutece]
    #23935003 - 12/17/16 10:25 AM (7 years, 1 month ago)

80-90% media water content, 1-5% soluble energy, soluble P, K, Mg, Zn. pH 5-6 at a guess.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
Invisiblefreespeech
disciple
 User Gallery


Registered: 12/12/08
Posts: 1,745
Loc: PNW
Re: Spore germination on agar [Re: The Mycologist]
    #23935004 - 12/17/16 10:26 AM (7 years, 1 month ago)

It can take a while. Be patient. I've heard that you want your agar to be more watery for spores from a print.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Spore germination on agar [Re: Ferather]
    #23935020 - 12/17/16 10:34 AM (7 years, 1 month ago)

they were done on 12/4


Quote:

Ferather said:
soluble P, K, Mg, Zn. pH 5-6 at a guess.





yah i got all that .....wtf is he taking about?? :awesanta:


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Spore germination on agar [Re: mushboy]
    #23935029 - 12/17/16 10:37 AM (7 years, 1 month ago)

More nutritional to germinate spores.
And slightly less stiff


Extras: Filter Print Post Top
Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 9 hours, 59 minutes
Re: Spore germination on agar [Re: mushboy]
    #23935032 - 12/17/16 10:38 AM (7 years, 1 month ago)




They like a wet mix for prints.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Spore germination on agar [Re: bodhisatta]
    #23935035 - 12/17/16 10:39 AM (7 years, 1 month ago)

Quote:

bodhisatta said:
More nutritional to germinate spores.
And slightly less stiff




thanks brostradamus.

i guess it will just take forever? ill make some new plates anyway.


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery


Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Spore germination on agar [Re: mushboy]
    #23935044 - 12/17/16 10:42 AM (7 years, 1 month ago)

Quote:

mushboy said:
any tips for germinating spores from a print on agar?(PDA)
make it less/more nutritious? it seems to be taking forever.

after doing a noobly job of scraping a chunk of spores off foil and onto a plate
i searched around and realized i should of used a qtip and done the ol' z pattern.
i dont think ive read they need to be hydrated in anyway.

im only curious because the same print was made into a syringe and those are growing out now.
they were made 3days after i did straight spores to agar. :shrug:




It's perfectly normal for spores from a print to take a week to germinate and 2 or 3 weeks is not unheard of.
A softer agar mix definitely helps, but the quality of the agar-agar you use has a lot to do with the ratio required to achieve a soft agar mix, it may vary from 1% to 1,5%.
Also, lots of people say that a higher nute ratio really helps with germination.

A chunk of the print to agar is an awful lot man :lol: Also, I wouldn't bother with streaking the q-tip  in a zig-zag pattern, just rub it softly on the center of the agar surface. Imo streaking in a zig-zag pattern is useful if you use an inoc loop to streak spore solution that's probably bacterial.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Spore germination on agar [Re: Josex]
    #23935051 - 12/17/16 10:44 AM (7 years, 1 month ago)

i havent worked with a print in ages. i forgot how easily they come off.
for some reason i remembered it being a pain.. who knows.

the only reason i did it that way was because i forgot to pc my qtips
and i just said fuck it.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Spore germination on agar [Re: mushboy]
    #23935076 - 12/17/16 10:56 AM (7 years, 1 month ago)

Mycelium have to digest, breakdown, energy and nutrients into small chunks, to absorb it through their cell wall.
Which is why I said soluble, less breaking down, more absorbing and thus improved speed and growth.

Next is the genetic ability to digest and the requirement to change digestive enzymes.
This is why some agar's work differently to others, various factors.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* spores germination on cardboard (pics) comario2 7,951 3 10/12/17 04:29 PM
by AK49
* Cracked corn & spore germination Erik006 1,802 5 08/19/03 04:25 PM
by Erik006
* Spores on agar question .More info plz tripndicular 4,346 5 09/13/02 05:27 PM
by tripndicular
* avg. spore germination time summitstealth 5,547 5 04/14/03 09:46 PM
by summitstealth
* About spore germination... lemming 3,008 3 11/05/02 02:23 PM
by tripndicular
* spore germination Anonymous 1,762 1 10/08/02 02:04 PM
by Skikid16
* Post deleted by Administrator HappyLeftist 729 4 01/07/03 04:50 PM
by Servator
* Will my year old spores germinate? case 2,625 2 08/16/01 02:49 PM
by shellacct

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,927 topic views. 30 members, 248 guests and 63 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 12 queries.