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Offlinelovelaughlibs
Dopamine Slave
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Registered: 10/14/15
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Can't seem to get germination outside of BRF?
    #23934910 - 12/17/16 09:40 AM (7 years, 1 month ago)

I purchased some syringes from a vendor and they seem to work fine on BRF cakes, but on both potato dextrose agar and rye grain I see nothing at all. On the plus side, no contamination, but after waiting a month I'm surprised to be seeing nothing.

How could I troubleshoot this or encourage germination?


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Invisibler.lutece
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Re: Can't seem to get germination outside of BRF? [Re: lovelaughlibs]
    #23934919 - 12/17/16 09:45 AM (7 years, 1 month ago)

Try making a wetter agar mix by reducing the amount of agar powder by .5g.


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OfflineTmethylM
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Re: Can't seem to get germination outside of BRF? [Re: r.lutece]
    #23935047 - 12/17/16 10:43 AM (7 years, 1 month ago)

Quote:

r.lutece said:
Try making a wetter agar mix by reducing the amount of agar powder by .5g.



Try that and also how long has it been since inoculation?


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Offlinelovelaughlibs
Dopamine Slave
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Registered: 10/14/15
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Re: Can't seem to get germination outside of BRF? [Re: Tmethyl]
    #23935522 - 12/17/16 01:57 PM (7 years, 1 month ago)

Cheers!

I've tried it a few times so far, maximum wait was 6 weeks but even that didn't work, I'll try the wetter PDA mix and see how it goes.


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OfflinecronicrFacebook
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Re: Can't seem to get germination outside of BRF? [Re: lovelaughlibs]
    #23935541 - 12/17/16 02:05 PM (7 years, 1 month ago)

Syringes to agar can be tricky..sometimes o resort to squirting the spores on a swab.


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OfflineFerather
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Registered: 03/19/15
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Re: Can't seem to get germination outside of BRF? [Re: cronicr]
    #23937110 - 12/18/16 02:19 AM (7 years, 1 month ago)

A square of kitchen towel, PC in a jar if you really need to.
Its enough to absorb a few drops of spore syringe.


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InvisibleMad Season
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Re: Can't seem to get germination outside of BRF? [Re: Ferather]
    #23937473 - 12/18/16 09:08 AM (7 years, 1 month ago)

Germinate in a pf jar/grain jar and transfer the mycelium to agar? Although for some reason I've never had issues with syringe to agar.

You can also stab under the surface and squirt a drop or 2 in :wink:


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