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bestpsybeever
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Registered: 10/11/15
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A question about senescence
#23933704 - 12/16/16 09:05 PM (7 years, 1 month ago) |
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I have a strain of GTs that I isolated a bit over a year ago. It is showing signs of senescence (slower mycelium growth, smaller/slower fruiting, and more contamination) but also another trait which I have not seen any information on. Since they've shown these signs of aging trips with them have been weird. 5g and I am still very aware of my ego, and the visuals have become extremely dull in color. not to say I'm not tripping hard, but the trips just aren't the same anymore.
I wanted to know if anybody else has some experience on this. Is it just a lack/difference in the alkaloids the shrooms are producing due to age? It could also be my brain changing, but it seems like a bit of a coincidence that these things have happened ate the same time.
-------------------- One thing I've learned. "You can turn your back on a person, but never on a drug, especially when it's waving a razor sharp hunting knife in your eye."
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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It is a cell division thing..always save your earlier plates.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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tump
ban the undead



Registered: 03/17/16
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Last seen: 6 years, 10 months
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Re: A question about senescence [Re: cronicr]
#23933761 - 12/16/16 09:32 PM (7 years, 1 month ago) |
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Yes save earlier plates. If you still want to save the culture. You challenge your myc, with weriod agar recipe like dog food or crickets. If you ever really love a culture on it second tranfers make a myc culture tube and store in 35f degrees. It will stay good for up ten years. Best bet now is to start over form a print to agar
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: A question about senescence [Re: tump]
#23933781 - 12/16/16 09:42 PM (7 years, 1 month ago) |
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Yup been there... Time to chunk that one. Slant early in the future to keep younger cell lines!
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AK1000
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Registered: 06/06/16
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While we're on the topic. What are the best teks for storing cultures for long term? (Any teks on how to make slants?)
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: A question about senescence [Re: AK1000]
#23933831 - 12/16/16 10:04 PM (7 years, 1 month ago) |
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Basically just mix your agar in a measuring cup... fill test tubes about half way... sterilize 30 minutes... Cool them at an angle... Inoculate... Loosen lid & wrap with film... Allow to colonize a week or so... Store in filtered jar in the fridge.
 
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AK1000
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Registered: 06/06/16
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Quote:
PirateSwazey said: Basically just mix your agar in a measuring cup... fill test tubes about half way... sterilize 30 minutes... Cool them at an angle... Inoculate... Loosen lid & wrap with film... Allow to colonize a week or so... Store in filtered jar in the fridge.
  
Sounds easy enough. Where do I buy said test tubes? (Got an amazon link or something?) And is there a slanted tray everyone uses? Or I guess it doesn't matter cause the agar cools slanted it just stays that way forever once it's cooled down.
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: A question about senescence [Re: AK1000]
#23933865 - 12/16/16 10:18 PM (7 years, 1 month ago) |
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It is easy as it sounds! Yeah Amazon has them... Just poke around you'll find em. Yes the agar cools at a slant and stays that way. Lay them on something clean at an angle.
Be sure to stir your agar between filling tubes to keep a homogenous mixture.
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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I notice your tubes are sitting in water in the jar. Why is that? Is it to prevent temperature fluctuations? Or does it keep them from drying out?
Edited by Dactylium (12/16/16 10:33 PM)
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: A question about senescence [Re: Dactylium]
#23933912 - 12/16/16 10:42 PM (7 years, 1 month ago) |
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Just for the sterilizer...you don't have to but with some tubes (like these plastic ones) it helps keep the agar from bubbling out.
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AK1000
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Registered: 06/06/16
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Quote:
PirateSwazey said: Just for the sterilizer...you don't have to but with some tubes (like these plastic ones) it helps keep the agar from bubbling out.
But won't that prevent it from being properly sterilized? I thought it was the steam and pressure that helps the temps go higher than boiling water allows. I wanna learn!
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: A question about senescence [Re: AK1000]
#23933938 - 12/16/16 11:13 PM (7 years, 1 month ago) |
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The temperature inside the sterilizer gets to 250 degrees. You could probably sterilize tubes for 20 minutes and still be fine... it's such a small amount of agar. The water in the jar helps stabilize the temperature inside the tubes so they don't pop or implode. It's not necessary but that's just how I learned to do it.
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bestpsybeever
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Registered: 10/11/15
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Last seen: 3 months, 11 days
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Thanks guys. I was thinking my weird trips were due to me to me tripping too much recently, good to know that it's probably just the shrooms.
Although this strain is unique in that it naturally mutated from regular gt to an albino gt (not a true albino, just pale caps.) I don't think there's anything I can do to rejuvenate it at this point, it seems pretty far gone. Looks like I'll just finish off what I've got left and start a new strain. I'll be excited to have shrooms the way they used to be again! 
Thanks for the info guys!
-------------------- One thing I've learned. "You can turn your back on a person, but never on a drug, especially when it's waving a razor sharp hunting knife in your eye."
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AK1000
Stranger



Registered: 06/06/16
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Last seen: 2 years, 5 months
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Quote:
bestpsybeever said: Thanks guys. I was thinking my weird trips were due to me to me tripping too much recently, good to know that it's probably just the shrooms.
Although this strain is unique in that it naturally mutated from regular gt to an albino gt (not a true albino, just pale caps.) I don't think there's anything I can do to rejuvenate it at this point, it seems pretty far gone. Looks like I'll just finish off what I've got left and start a new strain. I'll be excited to have shrooms the way they used to be again! 
Thanks for the info guys!
I wanna ask a question to the forum users here while we're on the topic:
If OP waits for some mushrooms to appear and makes a spore print of a mushroom and then grows it on some agar, will the senescence be "reset" or reverted back to square one cause it's starting with new genetics? Or are those spores full of genetic errors as well?
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: A question about senescence [Re: AK1000]
#23937404 - 12/18/16 08:28 AM (7 years, 1 month ago) |
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If I understand correctly, a spore print should reboot the genetic line. Like old people giving birth, kind of.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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bestpsybeever
Stranger

Registered: 10/11/15
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Re: A question about senescence [Re: r.lutece]
#23939265 - 12/18/16 09:27 PM (7 years, 1 month ago) |
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Yes, starting anew strain with a spore print from these old girls would "reset" the genetics of the strain... Well it would start a new strain, from multispore. Like new babies from a old lady. They should not have any of the genetic defects, nor any of the genetic advantages of the strain from which they were produced. It will be completely new.
I've just never found a spore print/syringe tek that I have been able to understand. Provably lack of attention on my part though.
Since I still have about 5 ml of spore syringe left I will try that first, and if it fails then I will try making a spore print.
-------------------- One thing I've learned. "You can turn your back on a person, but never on a drug, especially when it's waving a razor sharp hunting knife in your eye."
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Quote:
bestpsybeever said: Yes, starting anew strain with a spore print from these old girls would "reset" the genetics of the strain... Well it would start a new strain, from multispore. Like new babies from a old lady. They should not have any of the genetic defects, nor any of the genetic advantages of the strain from which they were produced. It will be completely new.
I've just never found a spore print/syringe tek that I have been able to understand. Provably lack of attention on my part though.
Since I still have about 5 ml of spore syringe left I will try that first, and if it fails then I will try making a spore print.
I haven't made a spore print myself personally, but a friend gave me one and I just made a make-shift inoculation loop using twisty-ties around a chopstick (here's the link to that tek) and just used that tool to scrub off a bunch of the spores and onto a pasty plate of agar and it germinated in a couple weeks. It was really easy. If it's a very old spore print, it has lost some viability and using a bit more spores may help ensure something grows.
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