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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Trich and Cubensis
#23933492 - 12/16/16 07:48 PM (7 years, 1 month ago) |
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Firstly I feel a bit weird about asking this, but I have multiple factors that could have contributed to this. I have never had trich before with my cubies, but in this batch I have had consistent poor results and have lost 5 out of 6 experimental tubs. This is what I am getting:

Possibility 1: My older pressure cooker was on its way out and there could have been residual bacteria in the jars of mycelium that I could not smell or see. This eventually weakened the mycelium and made the batch less resistant to mould?
Possibility 2: I had a good fan blowing in close proximity that could have blown an 'above normal' quantity of spores onto the substrate casing until it finally gave in?
Possibility 3: I used peat moss/ vermiculite casing and this is not ideal for cubies growing in a monotub?
I can't think of any other specific reasons why this has consistently happened? Any feedback appreciated
-------------------- Why reinvent the wheel when someone here has already done the science?
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Do you know if the trich is starting under the casting and emerging to the top, or starting directly atop the casing? I would try some without peat moss, I never had luck with it.
The fan should be used very minimally.
And I doubt your PC is the issue, they wouldn't have made it this far if so, you would see clear contamination in the jars.
-------------------- ¯\_(ツ)_/¯
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Re: Trich and Cubensis [Re: Tmethyl]
#23933759 - 12/16/16 09:31 PM (7 years, 1 month ago) |
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It definitely looked like it was on top of the casing. Did not want to dig it up too much and make a storm! Peat moss is great for pans, but what casing do you use for cubes? Last year I used Coco/verm 50/50 that worked fine.
-------------------- Why reinvent the wheel when someone here has already done the science?
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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In all honesty I don't case P.cubensis anymore, I know it's helpful sometimes but I find it entirely unnecessary, and one less vector for contamination. I started out casing then worked my way into uncased subs, and found no real value in casing, I was getting comparable weights and beautiful flushes both ways. Perfecting the growing conditions is more important than a casing for P.cubensis, I know Panaeolus species can be much more picky.
When I cased I used lightly moisturized 100% vermiculite. Crumbly.
-------------------- ¯\_(ツ)_/¯
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maxpassin
Shwad Scientist


Registered: 12/02/16
Posts: 116
Last seen: 5 years, 8 months
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Re: Trich and Cubensis [Re: Tmethyl]
#23933962 - 12/16/16 11:23 PM (7 years, 1 month ago) |
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Hmm, I have a question about contams like these (in a corner or on a side) on bulk trays. Is it salvageable at all?
In both scenarios (if the contam is just growing on the top / if it grew from the bottom up), would it be possible to just cut off a portion of the tray and save the rest? In your pic the contam takes up probably 4-6% of the whole tray right? what if you were to cut off like 20-30% of the tray to be safe? Could you save some of those pins?
I haven't attempted bulk trays yet, just wondering it it's possible.
Edited by maxpassin (12/16/16 11:23 PM)
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Trich and Cubensis [Re: maxpassin]
#23934042 - 12/16/16 11:52 PM (7 years, 1 month ago) |
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Quote:
maxpassin said: Hmm, I have a question about contams like these (in a corner or on a side) on bulk trays. Is it salvageable at all?
In both scenarios (if the contam is just growing on the top / if it grew from the bottom up), would it be possible to just cut off a portion of the tray and save the rest? In your pic the contam takes up probably 4-6% of the whole tray right? what if you were to cut off like 20-30% of the tray to be safe? Could you save some of those pins?
I haven't attempted bulk trays yet, just wondering it it's possible.
I have done it with success(removing contaminated sections with a knife), but I will always move the contaminated culture far away from my most active growing space. Sometimes once you remove the contam it will just continue as normal and produce plenty of fruits, other times the contams just take over and you have to toss it. Most molds are harmless and don't affect or infect the mushrooms whatsoever, but always post pics for trusted folks to tell you if what you have is safe.
-------------------- ¯\_(ツ)_/¯
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maxpassin
Shwad Scientist


Registered: 12/02/16
Posts: 116
Last seen: 5 years, 8 months
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Re: Trich and Cubensis [Re: Tmethyl]
#23934153 - 12/17/16 12:43 AM (7 years, 1 month ago) |
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Ah, so it's possible eh? cool thanks.
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Re: Trich and Cubensis [Re: maxpassin]
#23934817 - 12/17/16 08:56 AM (7 years, 1 month ago) |
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Oh damn, so I could have saved those trays? I thought trich was a deal breaker for sure and I had to bin the tray? Next time I will try and cut it out and hope that the disturbance of spores it creates is comparable with background levels that the mycelium can normally tolerate.
No casing at all on cubes growing in a monotub? Is that because the mycelium is so hardy that it protects the grain and serves as a barrier against fungal fallout?
-------------------- Why reinvent the wheel when someone here has already done the science?
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