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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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So one month after inoculation I get this.....Tric or potential?
#23933414 - 12/16/16 07:26 PM (7 years, 1 month ago) |
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Had almost given up on these plates as it had been so long this is the only one with any signs of life. I'm still a noob but my gut tells me this will just end up turning green and confirm that it is mould.
What do you guys think?
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
Edited by Dabrit (12/16/16 09:00 PM)
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Dabrit]
#23933425 - 12/16/16 07:30 PM (7 years, 1 month ago) |
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How did you inoculate? You can always transfer away from contams if there's anything good there.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: r.lutece]
#23933429 - 12/16/16 07:32 PM (7 years, 1 month ago) |
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Quote:
r.lutece said: How did you inoculate? You can always transfer away from contams if there's anything good there.
Spore syringe
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Dabrit]
#23933448 - 12/16/16 07:38 PM (7 years, 1 month ago) |
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Probably mold. Can't tell from pic / Pasty plate. Watch it grow and try another pic when it is bigger than a quarter.
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: So one month after inoculation I get this.....Tric or potential? [Re: PirateSwazey]
#23933510 - 12/16/16 07:54 PM (7 years, 1 month ago) |
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Would need a clearer photo to determine if it's mold or mushroom mycelium.
-------------------- ¯\_(ツ)_/¯
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Tmethyl]
#23933640 - 12/16/16 08:37 PM (7 years, 1 month ago) |
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Quote:
Tmethyl said:
Would need a clearer photo to determine if it's mold or mushroom mycelium.
Yeah its hard to get the camera on my phone to focus inside of the plate even with a good light on it. Will keep an eye on it for now and post another pic in a few days.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
Edited by Dabrit (12/16/16 08:38 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Dabrit]
#23933657 - 12/16/16 08:43 PM (7 years, 1 month ago) |
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if its been 1 month i highly doubt its myc. could be but i doubt it.
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: mushboy]
#23933698 - 12/16/16 09:02 PM (7 years, 1 month ago) |
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Quote:
mushboy said: if its been 1 month i highly doubt its myc. could be but i doubt it.
Yeah definitely have my doubts given the amount of time plus the syringe was over a year old that was used to inoculate it.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Dabrit]
#23933773 - 12/16/16 09:37 PM (7 years, 1 month ago) |
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It's not going to turn green. Wait for it to grow out more, then transfer away an outer edge piece to another clean plate. Good luck
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Psilosoulful]
#23933780 - 12/16/16 09:41 PM (7 years, 1 month ago) |
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trich looks like this on a plate. ...im guessing its trich??

it happened after i took some cuts. guess i wasnt careful
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Psilosoulful]
#23933786 - 12/16/16 09:45 PM (7 years, 1 month ago) |
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Personally I would think if it was mold it would have germinated much sooner. The fact that you didn't see anything for a whole month shows that you likely didn't get any contaminants on the plate and now the spores from the syringe are finally starting to germinate. It can take a while especially if the agar is a bit dry it can suck all the moisture off the surface where the spores are and delay germination.
I recently came up with a trick for germinating spores faster. place some spores on the agar and then cut a small square of agar out from a corner of the plate and sandwich the spores with it.
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Psilosoulful]
#23933876 - 12/16/16 10:22 PM (7 years, 1 month ago) |
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Quote:
Psilosoulful said: It's not going to turn green. Wait for it to grow out more, then transfer away an outer edge piece to another clean plate. Good luck
So you're saying you don't think it's tric?
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: mushboy]
#23933881 - 12/16/16 10:23 PM (7 years, 1 month ago) |
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Quote:
mushboy said: trich looks like this on a plate. ...im guessing its trich??

it happened after i took some cuts. guess i wasnt careful 
Yeah the white spot really does look similar to the spot in the top left of your picture difference is yours had turned green when the picture was taken and I'm thinking mine will as well.
But no matter have freshly inoculated plates which I hope pan out fingers crossed.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Thedillestpickle]
#23933886 - 12/16/16 10:24 PM (7 years, 1 month ago) |
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Quote:
Thedillestpickle said: Personally I would think if it was mold it would have germinated much sooner. The fact that you didn't see anything for a whole month shows that you likely didn't get any contaminants on the plate and now the spores from the syringe are finally starting to germinate. It can take a while especially if the agar is a bit dry it can suck all the moisture off the surface where the spores are and delay germination.
I recently came up with a trick for germinating spores faster. place some spores on the agar and then cut a small square of agar out from a corner of the plate and sandwich the spores with it.
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Thedillestpickle]
#23933907 - 12/16/16 10:34 PM (7 years, 1 month ago) |
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Quote:
Thedillestpickle said: Personally I would think if it was mold it would have germinated much sooner. The fact that you didn't see anything for a whole month shows that you likely didn't get any contaminants on the plate and now the spores from the syringe are finally starting to germinate. It can take a while especially if the agar is a bit dry it can suck all the moisture off the surface where the spores are and delay germination.
I recently came up with a trick for germinating spores faster. place some spores on the agar and then cut a small square of agar out from a corner of the plate and sandwich the spores with it.
Hmmm sounds like an interesting technique will keep in mind.
In terms of the agar I did follow PastyWhytes agar recipe(PastyPlates) to the letter for the plate shown.
I know a lot of people suggest using slightly less agar as I heard from a couple members stating that the recipe Pasty has( Pastyplates) may have been a little too stiff. So on my next set of petri's I just inoculated I used a half gram less of powdered agar. I think Pasty's recipe had non powdered agar in mind that you had to cut up or blend in a coffee grinder and not powdered agar so when he listed the weight in grams for powdered agar he likely guestimated how much weight you would need. So perhaps the stiff agar was what caused the delay or just the age of the spore syringe...or like I'm still leaning towards it's Trich
Time will tell.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Dabrit]
#23940366 - 12/19/16 01:37 PM (7 years, 1 month ago) |
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So Yeah.....looks like Tric-A-Whorea....
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Dabrit]
#23940402 - 12/19/16 01:54 PM (7 years, 1 month ago) |
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I guess it's obvious now.
-------------------- ¯\_(ツ)_/¯
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: So one month after inoculation I get this.....Tric or potential? [Re: Tmethyl]
#23940412 - 12/19/16 01:58 PM (7 years, 1 month ago) |
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Quote:
Tmethyl said: I guess it's obvious now. 
Haha yuup.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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