Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 2 months
Possible grain contamination?
    #23931372 - 12/16/16 01:10 AM (7 years, 2 months ago)

Swim recently innocculated 3 wheat berry spawn jars, its been 4 days and small bubbles have begun to form along with white (mycelium?) Is it too early to tell or is this a definite contamination?

Extras: Filter Print Post Top
InvisibleKush_Zombie
smug piece of shit
 User Gallery


Registered: 10/22/14
Posts: 4,793
Re: Possible grain contamination? [Re: Sullivanivan]
    #23931378 - 12/16/16 01:19 AM (7 years, 2 months ago)

I think you should quit worrying about people that aren't you.

post pics 'someone who is me'


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.

Extras: Filter Print Post Top
InvisibleNobler Hino
a dojo and a forge?!
 User Gallery

Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Kush_Zombie]
    #23931473 - 12/16/16 03:15 AM (7 years, 2 months ago)

You didnt even say anything illegal lol

Pics would help


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina

Extras: Filter Print Post Top
OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 2 months
Re: Possible grain contamination? [Re: Nobler Hino]
    #23931732 - 12/16/16 08:44 AM (7 years, 2 months ago)

Lesson learned XD


Extras: Filter Print Post Top
InvisibleNobler Hino
a dojo and a forge?!
 User Gallery

Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932564 - 12/16/16 01:58 PM (7 years, 2 months ago)

Ew bacteria, how did you inoculate?


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina

Extras: Filter Print Post Top
OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 2 months
Re: Possible grain contamination? [Re: Nobler Hino]
    #23932577 - 12/16/16 02:02 PM (7 years, 2 months ago)

Using a diy hood from a plastic container, I pc'ed the jars for 90 minutes and sterilized the syringe tip before, the grain was soaked for 24 hours beforehand for endospores as well

Extras: Filter Print Post Top
OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 2 months
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932591 - 12/16/16 02:08 PM (7 years, 2 months ago)

Also, the spores are from a questionable distributor, is it possible they were bad from the start?

Extras: Filter Print Post Top
InvisibleNobler Hino
a dojo and a forge?!
 User Gallery

Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932628 - 12/16/16 02:26 PM (7 years, 2 months ago)

24 hours is too long, soak for 12 hours.

We soak our grains to saturate the inside of the grains so they dont burst when we simmer them. The pressure cooker is the one to take care of endospores. (Wbs doesnt need a simmer, just a soak.)

MS syringe to grains is a bad idea, you really need to check it on agar first for contams, then drop a wedge.

I think your discribing a still air box, that was a good move.


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina

Edited by Nobler Hino (12/16/16 02:27 PM)

Extras: Filter Print Post Top
InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932720 - 12/16/16 02:56 PM (7 years, 2 months ago)

Quote:

Sullivanivan said:
Also, the spores are from a questionable distributor, is it possible they were bad from the start?




Yes.

Find a better vendor or learn agar. Ideally both.

Extras: Filter Print Post Top
OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 2 months
Re: Possible grain contamination? [Re: TheEaglesGift]
    #23932736 - 12/16/16 03:07 PM (7 years, 2 months ago)

Yes an air box, soaking for 12 hours, the agar makes sense, I'm anxious to try again, thank you for the help

Extras: Filter Print Post Top
InvisibleNobler Hino
a dojo and a forge?!
 User Gallery

Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932752 - 12/16/16 03:14 PM (7 years, 2 months ago)

No prob bro


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* Grain tek/endospores related Q GnuBobo 1,129 2 06/30/04 04:25 PM
by GnuBobo
* Endospores--everywhere? GnuBobo 1,289 3 07/01/04 03:30 PM
by GnuBobo
* Post deleted by MOE THE MAD SCIENTIST
( 1 2 all )
vatoloco 10,096 21 04/30/02 03:27 AM
by Zebedee
* WBS endospores Gumby79 2,307 7 03/01/04 09:36 PM
by Gumby79
* What causes the contam Bacillus besides endospores? MOTH 1,818 2 05/15/04 03:47 AM
by ATWAR
* what kind of contamination is this ? (PIC) ragadinks 2,188 6 12/22/03 06:03 PM
by ragadinks
* B. anthracis contamination? C_Dog1 1,144 3 09/07/04 08:02 PM
by C_Dog1
* Purple/Pink contamination can be DEADLY
( 1 2 3 all )
Ryche HawkV 32,603 42 04/23/22 11:05 PM
by Imatryin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
336 topic views. 23 members, 137 guests and 31 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.006 seconds on 12 queries.