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OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 1 month
Possible grain contamination?
    #23931372 - 12/16/16 01:10 AM (7 years, 1 month ago)

Swim recently innocculated 3 wheat berry spawn jars, its been 4 days and small bubbles have begun to form along with white (mycelium?) Is it too early to tell or is this a definite contamination?


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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: Possible grain contamination? [Re: Sullivanivan]
    #23931378 - 12/16/16 01:19 AM (7 years, 1 month ago)

I think you should quit worrying about people that aren't you.

post pics 'someone who is me'


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How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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InvisibleNobler Hino
a dojo and a forge?!
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Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Kush_Zombie]
    #23931473 - 12/16/16 03:15 AM (7 years, 1 month ago)

You didnt even say anything illegal lol

Pics would help


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"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 1 month
Re: Possible grain contamination? [Re: Nobler Hino]
    #23931732 - 12/16/16 08:44 AM (7 years, 1 month ago)

Lesson learned XD



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InvisibleNobler Hino
a dojo and a forge?!
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Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932564 - 12/16/16 01:58 PM (7 years, 1 month ago)

Ew bacteria, how did you inoculate?


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 1 month
Re: Possible grain contamination? [Re: Nobler Hino]
    #23932577 - 12/16/16 02:02 PM (7 years, 1 month ago)

Using a diy hood from a plastic container, I pc'ed the jars for 90 minutes and sterilized the syringe tip before, the grain was soaked for 24 hours beforehand for endospores as well


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OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 1 month
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932591 - 12/16/16 02:08 PM (7 years, 1 month ago)

Also, the spores are from a questionable distributor, is it possible they were bad from the start?


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InvisibleNobler Hino
a dojo and a forge?!
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Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932628 - 12/16/16 02:26 PM (7 years, 1 month ago)

24 hours is too long, soak for 12 hours.

We soak our grains to saturate the inside of the grains so they dont burst when we simmer them. The pressure cooker is the one to take care of endospores. (Wbs doesnt need a simmer, just a soak.)

MS syringe to grains is a bad idea, you really need to check it on agar first for contams, then drop a wedge.

I think your discribing a still air box, that was a good move.


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


Edited by Nobler Hino (12/16/16 02:27 PM)


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932720 - 12/16/16 02:56 PM (7 years, 1 month ago)

Quote:

Sullivanivan said:
Also, the spores are from a questionable distributor, is it possible they were bad from the start?




Yes.

Find a better vendor or learn agar. Ideally both.


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OfflineSullivanivan
Stranger
Registered: 12/16/16
Posts: 5
Last seen: 7 years, 1 month
Re: Possible grain contamination? [Re: TheEaglesGift]
    #23932736 - 12/16/16 03:07 PM (7 years, 1 month ago)

Yes an air box, soaking for 12 hours, the agar makes sense, I'm anxious to try again, thank you for the help


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InvisibleNobler Hino
a dojo and a forge?!
 User Gallery

Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Possible grain contamination? [Re: Sullivanivan]
    #23932752 - 12/16/16 03:14 PM (7 years, 1 month ago)

No prob bro


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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