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Peyote Road
Stranger

Registered: 09/02/15
Posts: 3,527
Loc: Great Lakes State
Last seen: 1 year, 3 months
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help after failed grows
#23930835 - 12/15/16 08:31 PM (7 years, 1 month ago) |
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I am no good at growing mushrooms apparently. My first couple grows went great even despite the fact that I had no idea what I was doing. THis made me think growing mushrooms was super easy. But since then I have had problem after problem, from green mold taking over the cakes to bad spores, to contamination which seems to be my biggest issue of as late.
I dont understand why in my first grows I followed the same procedure and only had one jar contaminate. Now, my jars are contaminated after only 3 days after planing the spores! They contaminate before the mycellium even begins to grow! Which each of my last three grows the jars have contaminated with this gray orgranism and in each subsequent grow it happens quicker. FIrst time they contamed after about 2 weeks, second time after about a week and this most recent time in only 3 days!
Can someone tell me what I am doing wrong? I am using pf tek, brown rice flour and vermiculite cakes pressure cook on medium heat for 45 minutes and inoculate using the oven method wearing a mask and sterilazing syringe after every 2 jars.
-------------------- The path of the herbalist is to open ourselves to nature in an innocent and pure way. SHe in turn will open her bounty and reward us with many valuable secrets. May the earth bless you. - Michael Tierra
Edited by Peyote Road (12/15/16 08:32 PM)
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Are you inoculating in open air? Build a still air box if you are. Other than that there isn't much you can do, syringes are dirty. It's luck of the draw with PF tek.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Oven method is not a good practice, it actually pulls room air toward it and circulates it. Use a sab or a glove box. It could also be the syringe/s you are using are very dirty. Have you changed vendor's or anything?
Edited by PinPornProducer (12/15/16 08:39 PM)
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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I fucked up my last 2 pf grows. Said fuck it and attempted bulk and almost all of my grows have been solid since. Must've been the mushrooms telling me to stop thinking small
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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PC at least an hour bro.
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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even pf needs 90min at 15psi. when i steamed them i did 3hours. 45min isnt nearly enough time dude.
and you flame sterilize after every jar.
and dont do the oven tek shit
a sab box require zero thought power. just brawn.
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tripo3
Trippy-Hippo


Registered: 04/02/16
Posts: 324
Last seen: 5 years, 5 days
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Re: help after failed grows [Re: mushboy]
#23930899 - 12/15/16 08:56 PM (7 years, 1 month ago) |
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along with what everyone said make sure your spores are clean and coming from a well trusted vendor.
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unfortunategent
Stranger


Registered: 06/04/14
Posts: 316
Last seen: 2 years, 2 months
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A few easy things you could look into:
1.) Buy a 66qt plastic tote and cut holes big enough for your arms to fit in. Do all your sterile work here.
2.) Make sure you're using your pressure cooker correctly. I turn mine on high, getting a steady flow of steam for about 10 min, then turn it down until it reaches a steady pressure...then I start counting down.
3.) Flame sterilize after every jar.
4.) Don't count on the syringes being clean. Make pasty plates (takes no time at all--10 min. of work/an hour of cooking/a few hrs to cool) or order some pre poured agar plates to drop spores on. This way you'll know exactly what you're inoculating with as you'll see any contaminants clearly.
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unfortunategent
Stranger


Registered: 06/04/14
Posts: 316
Last seen: 2 years, 2 months
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also 45 min of proper pressure cooking should be enough. If you're just steaming, then you'll need 90 min
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: help after failed grows [Re: tripo3]
#23930913 - 12/15/16 09:01 PM (7 years, 1 month ago) |
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Quote:
tripo3 said: along with what everyone said make sure your spores are clean and coming from a well trusted vendor.
Does she know she's an ad? DOES SHE KNOW SHE'S AN AD??
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Basically
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
unfortunategent said: also 45 min of proper pressure cooking should be enough. If you're just steaming, then you'll need 90 min
nah. go with 90 in the pc please.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: help after failed grows [Re: mushboy]
#23930944 - 12/15/16 09:09 PM (7 years, 1 month ago) |
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Quote:
mushboy said:
Quote:
unfortunategent said: also 45 min of proper pressure cooking should be enough. If you're just steaming, then you'll need 90 min
nah. go with 90 in the pc please.
Good call mushboy. Don't know how I missed that. Why on earth would you ever reccomend a shorter PC cycle to sone one having contamination issues? And fyi 60 is bare minimum. 90+ is reccomended
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
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1. If you don't have a pressure cooker, get one 2. Make a still air box and use it These two steps eliminate most problems. 3. I never found PF Tek an easy method at all ... others do and I understand that ... Having said that, if your cakes are contaminating, you've got bad spores or you aren't sterilizing your cakes properly. 4. Try V-tek method ... https://www.shroomery.org/forums/showflat.php/Number/21390984 ... as it's the easiest method I found. 5. Learn how to use agar ... pasty-plates are easy and cheap ... as that will dramatically reduce your contamination risk.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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unfortunategent
Stranger


Registered: 06/04/14
Posts: 316
Last seen: 2 years, 2 months
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Quote:
ComebackKid said:
Quote:
mushboy said:
Quote:
unfortunategent said: also 45 min of proper pressure cooking should be enough. If you're just steaming, then you'll need 90 min
nah. go with 90 in the pc please.
Good call mushboy. Don't know how I missed that. Why on earth would you ever reccomend a shorter PC cycle to sone one having contamination issues? And fyi 60 is bare minimum. 90+ is reccomended
You guys are right. 45 min is not enough time, so thanks for correcting me. I meant to say 60 min, as that's what I have always done for cakes in the past. Haven't done cakes in awhile though. OP, I think if you make the changes listed in the few replies you've received so far, you will see some positive results.
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tripo3
Trippy-Hippo


Registered: 04/02/16
Posts: 324
Last seen: 5 years, 5 days
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ad?
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