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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Question regarding creating G2G Master Jars
#23929416 - 12/15/16 01:30 PM (7 years, 1 month ago) |
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Have a question in regards as to method fo going from inoculated agar to creating 6 spawn jars per monotub.
Right now I have 5 inoculated petri dishes of PE( 5th of which I wasn't able to pour a full dish but figured will work none the less) and I have 4 inoculated petri dishes of GT's( this time got my pour method down pat and all 4 look good).
Am wondering which way to go about things in terms of going from agar to creating my 6 jars per variety of spawn.
Obviously first I know I need to obtain at least one clean culture per variety.
Do I then say:
1) grow out the healthy cultures a fair amount and use the best looking plates and then PC 6-8( two extra in case of contamination) grain jars per variety and then divide up one agar plate between the jars?
or 2) Should I grow out the best plate per variety and create two master jars per variety( again the 1 extra in case of contamination) and then once the two masters are fully colonized do G2G transfers to get my 6 healthy spawn jars per variety and then perhaps store the rest of the healthy agar plates in the fridge for later use?
I'm thinking perhaps the second method will get me better timing with my jars? Also I can hold onto more of my clean culture this way. Although I would be looking to clone onto agar once I have fruited out the two tubs. However it could turn out that the tubs are bunk and lack potency or are lost to contamination.
any insight would be much appreciated.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Question regarding creating G2G Master Jars [Re: Dabrit]
#23929423 - 12/15/16 01:34 PM (7 years, 1 month ago) |
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I'll usually use one plate to inoculate 2-3 grain masters for expansion. If your concern is contamination, the safest route would be to use one plate per grain master.
G2G each grain master into 4-6 recieving jars for your spawn run.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Question regarding creating G2G Master Jars [Re: PirateSwazey]
#23929503 - 12/15/16 01:57 PM (7 years, 1 month ago) |
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make 8 masters
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: Question regarding creating G2G Master Jars [Re: cronicr]
#23929532 - 12/15/16 02:09 PM (7 years, 1 month ago) |
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Quote:
cronicr said: make 8 masters
Funny guy one day perhaps I'll have room for that level of expansion.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Question regarding creating G2G Master Jars [Re: Dabrit]
#23929543 - 12/15/16 02:11 PM (7 years, 1 month ago) |
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well if all 8 work out you have 6 to spawn and two to expand
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Re: Question regarding creating G2G Master Jars [Re: cronicr]
#23929566 - 12/15/16 02:19 PM (7 years, 1 month ago) |
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I'd save a small chunk of agar to put on another plate.
I do that so I always have some clean agar to work with....
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: Question regarding creating G2G Master Jars [Re: cronicr]
#23929588 - 12/15/16 02:27 PM (7 years, 1 month ago) |
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Quote:
cronicr said: well if all 8 work out you have 6 to spawn and two to expand
Oh shit sorry I misunderstood what you said. I thought you meant create 8 additional masters from my first two masters.
So basically what you're saying is what I have for option #1 wouldn't the problem be though that without fruiting it I don't have any idea if the spawn will supply a potent flush or potentially when spawning to bulk could get contaminated and then I've lost all of my clean agar culture...not that it matters I guess still have lots left in the spore syringe.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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keifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
Last seen: 26 days, 8 hours
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Re: Question regarding creating G2G Master Jars [Re: Dabrit]
#23929812 - 12/15/16 03:33 PM (7 years, 1 month ago) |
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You could cut one plate to knock up 8 jars but it will be slower then if you use 1 plater per jar master..
You could turn the plater into a LI and knock up more jars but I find it better to do LI with a cornmeal plate.. I say do 1 plate per jar or cut a plate into 6 sections and knock up 2 masters
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: Question regarding creating G2G Master Jars [Re: keifnnugs]
#23929843 - 12/15/16 03:42 PM (7 years, 1 month ago) |
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Quote:
keifnnugs said: You could cut one plate to knock up 8 jars but it will be slower then if you use 1 plater per jar master..
You could turn the plater into a LI and knock up more jars but I find it better to do LI with a cornmeal plate.. I say do 1 plate per jar or cut a plate into 6 sections and knock up 2 masters
Word.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Re: Question regarding creating G2G Master Jars [Re: Dabrit]
#23930257 - 12/15/16 05:37 PM (7 years, 1 month ago) |
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I thought that melting TV in your signature was a flowhood at first. I spend too much time here.
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: Question regarding creating G2G Master Jars [Re: Dactylium]
#23930436 - 12/15/16 06:32 PM (7 years, 1 month ago) |
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Quote:
Dactylium said: I thought that melting TV in your signature was a flowhood at first. I spend too much time here.
To be honest it actually really does resemble a flow hood...it can be whatever you want it to be as long as it's psychedelic in nature.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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