Home | Community | Message Board

Cannabis Seeds Zamnesia
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Left Coast Kratom Buy Kratom Capsules   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: < First | < Back | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 7 months
Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Munchauzen]
    #23396149 - 06/30/16 01:30 AM (7 years, 10 months ago)

Quote:

Munchauzen said:
Quote:

cj711 said:
Quote:

blackout said:
Quote:

cj711 said:MS syringes bought from vendors and always inoculate each jar with about 1-1.5 CC's of solution.



They also do not want loads of competing growth from loads of germinating spores.






why??? isn't that why agar is so awesome, because it has so many germinating spores and thus makes colonization times faster?? seems contradictory to me...



nothing of what you just said made any sense.




Eh, it kind of does, but it's wrong. The point of agar is to separate growth and remove some of the randomness in the genetics. You aren't just trying to colonize the plate as fast as possible. The point is to verify that there are no contaminants and find good genetics that you can separate from the rest.

Extras: Filter Print Post Top
Offlineblackout
 User Gallery

Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 9 days
Trusted Cultivator
Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: spacechildo]
    #23396622 - 06/30/16 07:19 AM (7 years, 10 months ago)

Quote:

spacechildo said:
Quote:

blackout said:
I have completely dried out grains, drier than they were in their natural state, and added all the moisture back in as LC.




that sounds crazy! :lol: did you document it?




Here are 2 I can find
https://www.shroomery.org/forums/showflat.php/Number/4685380

this is my sig link, not sure if they were dried to completion.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=4294326

Extras: Filter Print Post Top
OfflineLa Flama Blanca
Stranger
 User Gallery
Registered: 01/15/16
Posts: 487
Last seen: 1 year, 2 months
Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: GreenRabbit]
    #23396745 - 06/30/16 08:08 AM (7 years, 10 months ago)

Quote:

GreenRabbit said:


Eh, it kind of does, but it's wrong. The point of agar is to separate growth and remove some of the randomness in the genetics. You aren't just trying to colonize the plate as fast as possible. The point is to verify that there are no contaminants and find good genetics that you can separate from the rest.




yes, that much I do know, but i swear i saw in i think the tiger drop tek, the guy said the tiger drop method is so good also because it gives so many "inoculation points" and thus will colonize extremely fast. i guess inoculation points does not equal spores though

Extras: Filter Print Post Top
InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: La Flama Blanca]
    #23396755 - 06/30/16 08:12 AM (7 years, 10 months ago)

The tiger drop is only 1 inoculation point, it is just very large. But no, inoculating with agar is definitely not the same as using spores. It will still be in effect, a multispore grow but no spores are actually used to inoculate the grains. The spores are germinated on agar so the cultures can be cleaned up from any possible contams that were on the spores. You are just using that multistrain mycelium to inoculate your grains with.

Extras: Filter Print Post Top
OfflineLa Flama Blanca
Stranger
 User Gallery
Registered: 01/15/16
Posts: 487
Last seen: 1 year, 2 months
Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Supalemonhaze]
    #23396916 - 06/30/16 09:29 AM (7 years, 10 months ago)

ohhhh i get it i think thank you. the spores germinate whatever nutrition you apply them to first, then mature/evolve into the myc growth from that point, no more spores are being created; just mycelia. cool!

Extras: Filter Print Post Top
OfflineMachiavelliavore
Vermiculite Hater
 User Gallery


Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 7 months, 5 days
Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: La Flama Blanca]
    #23397832 - 06/30/16 03:02 PM (7 years, 10 months ago)

A tigerdrop can be between a single and many inoculation points.  If you just drop a single wedge in and leave it, growth will emanate from that wedge.  If you shake it hard, mycellium will be scraped off and the wedge will probably fall apart creating far more inoculation points.  Liquid Inoculant (blended agar plate in water) has far higher inoculation points still.  The tigerdrop 2.0 (chopped agar plate) aids in spreading mycellium and pieces of wedge.

But as others said, the point of agar is to know what you're inoculating with (ie clean culture/monoculture.), not to go faster.  Multispore can have quite a nice inoculation point spread if you shake a good amount, but it will tend to be slower since you have spore germination time tagged on. People tend not to shake MS syringe inoculations immediately so they can inspect the growth on the side of the jar, then shake and effectively g2g the rest of the jar.  MS Syringe to Grain will almost always outpace MS Syringe to Agar to Grain, but is less reliable.  Live mycellium to agar to grain should be comparable or faster than MS syringe to grain and more reliable.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...

Extras: Filter Print Post Top
OfflineBoogens
Boogens


Registered: 06/18/15
Posts: 96
Loc: Canada Flag
Last seen: 3 years, 2 months
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Violet]
    #23407122 - 07/03/16 01:17 PM (7 years, 10 months ago)

Hi Violet,

I have contams. How should I go about dealing with the containers?

Should I crack them and steam sterilise for a couple hours then dump the contents?


--------------------


            How it should & shouldn't look - NEW CULTIVATORS GUIDE

                          FAQ's

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,866
Loc: Canada Flag
Trusted Cultivator
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Boogens]
    #23407125 - 07/03/16 01:18 PM (7 years, 10 months ago)

Yup. Steam, dump, do better next time.

Extras: Filter Print Post Top
OfflineBoogens
Boogens


Registered: 06/18/15
Posts: 96
Loc: Canada Flag
Last seen: 3 years, 2 months
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Pastywhyte]
    #23407134 - 07/03/16 01:21 PM (7 years, 10 months ago)

Thanks bro


--------------------


            How it should & shouldn't look - NEW CULTIVATORS GUIDE

                          FAQ's

Extras: Filter Print Post Top
Offlinesecrethq
Stranger
 User Gallery
Registered: 10/27/16
Posts: 7
Last seen: 7 years, 2 months
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Violet]
    #23813992 - 11/08/16 08:29 PM (7 years, 6 months ago)

I just have one little simple question... Should I shake right before inoculation, just to break everything up a little? I'm trying to follow this tek to the letter but haven't found the answer in the first 20 pages. I'm using grass seed. I'm a noob, go easy on me lol!

Edited by secrethq (11/08/16 08:30 PM)

Extras: Filter Print Post Top
InvisibleViolet
 User Gallery


Registered: 12/06/11
Posts: 4,205
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: secrethq]
    #23816891 - 11/09/16 05:03 PM (7 years, 6 months ago)

Yeah that's a fine idea. Distribute any standing moisture and settle the seeds down evenly.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts

Extras: Filter Print Post Top
Offlineeritain
Stranger
Registered: 11/12/15
Posts: 1
Last seen: 7 years, 5 months
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Violet]
    #23827127 - 11/12/16 08:10 PM (7 years, 6 months ago)

Quote:

Violet said:





Looks like those hit full growth exactly at the top of the container. Very nice use of space!

... well, they do look kind of skinny though. Maybe the yield per container would be better with fatasses? Hmm. Has anyone experimented with this?

I guess if you could get something to grow skinny but close-packed, like enoki, that would be perfect. But unlikely. :smile:

Extras: Filter Print Post Top
InvisibleViolet
 User Gallery


Registered: 12/06/11
Posts: 4,205
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: eritain]
    #23827387 - 11/12/16 09:34 PM (7 years, 6 months ago)

Lots of grow look similar to them. The methods can effect how genes end up looking sometimes but tall mushrooms aren't a detriment. The grow still gets nice yield for the substrate, depending on the culture of course.
When testing cultures to find which one you want to use, you'll end up picking the one that makes the most by weight. Might be fat, may be tall and dense clusters - what I like.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts

Extras: Filter Print Post Top
OfflineMacondo
Stranger
Registered: 12/15/16
Posts: 4
Last seen: 7 years, 1 month
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Violet]
    #23928432 - 12/15/16 06:14 AM (7 years, 5 months ago)

This tek looks pretty amazing so I decided to try it out. I've got ten containers going at various stages, anywhere from 30% to 80% colonized. I have a question about adding water, do you need to use sterilized water or just tap water? I read through the full thread twice and I don't think this was specifically mentioned, or I missed it!

Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery


Registered: 11/13/15
Posts: 8,998
Loc: Flag
Trusted Cultivator
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Macondo]
    #23928437 - 12/15/16 06:19 AM (7 years, 5 months ago)

Tap water is fine. The second you expose sterile water to open air it's no longer sterile, so it'd be pointless to do that anyway.

Extras: Filter Print Post Top
InvisibleKauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Macondo]
    #23928444 - 12/15/16 06:31 AM (7 years, 5 months ago)

Quote:

Macondo said:
This tek looks pretty amazing so I decided to try it out. I've got ten containers going at various stages, anywhere from 30% to 80% colonized. I have a question about adding water, do you need to use sterilized water or just tap water? I read through the full thread twice and I don't think this was specifically mentioned, or I missed it!




I've used this tek extensively and bottom watering is essential.  What I do is I boil a full pot of water and then keep it in a tea kettle that holds about a liter, I suspect and use that water to bottom water that jars.  It's certainly not sterile, but it's pretty clean and there is no reason to introduce any additional contams into the jar if it can be avoided.  Certainly tap water will work, but I feel better with water that's been boiled.  The kettle I use has a spout that's perfect for pouring small amounts of water into the jars. 

Also, I've found that, at least with the strains I grow, once they grow beyond the small pin size, some additional air really helps.  They will grow with the lids on, but will grow MUCH BIGGER if you take the lids off from time to time or create a different "hood" out of a 2-liter plastic soda bottle ... this gives them more headroom to grow and more air ventilation. 

However, if you're using the method to farm some near sterile pins, that's another story.  I do that too and don't take the lid off until some harvestable pins show up and then I nab them, put them on Agar (this is how to find a great culture, which is essential) and then let the jar grow out as normal.

It is critical to understand that all strains will not grow well in these conditions.  You have to find one that will and once you do, you're golden.  I found that South Americans and Amazonians thrive in these conditions, moreso than other strains I've tried.  Not to say you can't find a great culture from ANY strain that will work if you try hard enough, but it  helps to start with one that has a high likelihood of fruiting in invitro conditions and regardless of what anyone says, a lot of strains will fruit fine in SGFC's or Monotubs, but NOT invitro.  Common sense things like misting to keep the top of the substrate from drying out apply as well. 


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master

Edited by KauaiOrca (12/15/16 07:37 AM)

Extras: Filter Print Post Top
OfflineMacondo
Stranger
Registered: 12/15/16
Posts: 4
Last seen: 7 years, 1 month
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Josex]
    #23928506 - 12/15/16 07:26 AM (7 years, 5 months ago)

Quote:

Josex said:
Tap water is fine. The second you expose sterile water to open air it's no longer sterile, so it'd be pointless to do that anyway.




Thanks Josex, that makes sense, I kinda figured also that if the seed is fully colonized, it's not as likely to contaminate anyway.

Extras: Filter Print Post Top
OfflineMacondo
Stranger
Registered: 12/15/16
Posts: 4
Last seen: 7 years, 1 month
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: KauaiOrca]
    #23928524 - 12/15/16 07:42 AM (7 years, 5 months ago)

Quote:

KauaiOrca said:
I've used this tek extensively and bottom watering is essential.  What I do is I boil a full pot of water and then keep it in a tea kettle that holds about a liter, I suspect and use that water to bottom water that jars.  It's certainly not sterile, but it's pretty clean and there is no reason to introduce any additional contams into the jar if it can be avoided.  Certainly tap water will work, but I feel better with water that's been boiled.  The kettle I use has a spout that's perfect for pouring small amounts of water into the jars. 

Also, I've found that, at least with the strains I grow, once they grow beyond the small pin size, some additional air really helps.  They will grow with the lids on, but will grow MUCH BIGGER if you take the lids off from time to time or create a different "hood" out of a 2-liter plastic soda bottle ... this gives them more headroom to grow and more air ventilation. 

However, if you're using the method to farm some near sterile pins, that's another story.  I do that too and don't take the lid off until some harvestable pins show up and then I nab them, put them on Agar (this is how to find a great culture, which is essential) and then let the jar grow out as normal.

It is critical to understand that all strains will not grow well in these conditions.  You have to find one that will and once you do, you're golden.  I found that South Americans and Amazonians thrive in these conditions, moreso than other strains I've tried.  Not to say you can't find a great culture from ANY strain that will work if you try hard enough, but it  helps to start with one that has a high likelihood of fruiting in invitro conditions and regardless of what anyone says, a lot of strains will fruit fine in SGFC's or Monotubs, but NOT invitro.  Common sense things like misting to keep the top of the substrate from drying out apply as well. 




Great advice! I like the tea kettle idea.

I'm not sure how these will fruit invitro.  I have only done one grow using the PF Tek, mostly with Burma strain,  but two cakes with Amazon.  I was down to the final flush and ran into problems trying to buy more spore syringes, so I made up a few agar jars and cloned the very last shroom. Unfortunately I am not 100% sure which one I cloned (I learned my lesson and now label the hell out of everything!)  At least I know that all ten containers are from the same clone, so if I have trouble fruiting the first ones, I can move the others into my SGFC.

Extras: Filter Print Post Top
InvisibleKauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: Macondo]
    #23928569 - 12/15/16 08:14 AM (7 years, 5 months ago)

Quote:

Macondo said:

Great advice! I like the tea kettle idea.

I'm not sure how these will fruit invitro.  I have only done one grow using the PF Tek, mostly with Burma strain,  but two cakes with Amazon.  I was down to the final flush and ran into problems trying to buy more spore syringes, so I made up a few agar jars and cloned the very last shroom. Unfortunately I am not 100% sure which one I cloned (I learned my lesson and now label the hell out of everything!)  At least I know that all ten containers are from the same clone, so if I have trouble fruiting the first ones, I can move the others into my SGFC.




This is the most important element of invitro growing, from my experience.  If you look at Violet's tek of doing some small jars of RGS and then snagging the good pins to start a high potential/probability culture, you're way ahead.  I personally think invitro is a great growing method because it's stealthy, super easy, very contam free and you don't need big fruiting chambers and they can produce very efficiently too.  I highly suggest you start some small pint jars with about a half inch of RGS in them, use whatever LC you have and get 3-4 of them going then work on finding great pins.  Once you get that, you'll love this system.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master

Extras: Filter Print Post Top
OfflineMacondo
Stranger
Registered: 12/15/16
Posts: 4
Last seen: 7 years, 1 month
Re: PODS TEK – Invitro tek growing with BRF or Grass Seed: a simple, self-contained, single-step method [Re: KauaiOrca]
    #23930432 - 12/15/16 06:31 PM (7 years, 4 months ago)

Thanks for all the advice KauaiOrca.  I am going to be doing a G2G of Ecuador strain this weekend. Sounds like tht may be a better choice. If I can get them to fruit then I'll definitely get them into the RGS and get a good culture.

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | Next > | Last >

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Left Coast Kratom Buy Kratom Capsules   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Do-It-Yourself Pod Tek
( 1 2 all )
Drummer 6,281 21 06/07/02 05:43 PM
by Mycelium69
* Wick water feed for Inner Resevoir Tek? BlueX 1,367 4 10/08/01 12:21 AM
by Rashiki
* Gulf Coast Invitro... Shiznitz 2,485 9 08/14/01 11:48 PM
by psilocybinjunkie
* need help.. neglect tek max 2,180 8 07/27/01 07:39 AM
by Vitticeps
* rye tek question yogithehoneybear 1,696 2 01/24/02 07:00 AM
by psilocyber
* Good tek for a dorm room??? happycamper 2,194 10 07/01/02 01:44 PM
by GreyMatter
* Re: Invitro EQ Pics Holydiver 1,435 3 04/13/01 08:33 AM
by Teragon
* eating the mycelium (Foolproof Tek w/ BRF)?????? matamore 4,650 8 10/04/18 04:10 PM
by bodhisatta

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
115,919 topic views. 26 members, 118 guests and 64 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.009 seconds on 14 queries.