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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Agar pin questions
#23925816 - 12/14/16 12:25 PM (7 years, 1 month ago) |
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Gotta couple of questions about strains in agar pins.
1. A clone taken from a fruited multispore grow will have multiple strains. So a pin grown frown MS to agar should also have multiple strains, correct?
2. (If #1 is true:) Agar pins are cloned to move forward with a strain that is a proven fruiter. If a cloned pin has multiple strains, are all of the strains found in the pin proven fruiters? Or is only one guaranteed to be a proven fruiter?
3. If more than one pin forms on agar, is it more likely that pin A is formed out of different strains than pin B, or that they share some or all of the same strains? Alternative phrasing: when multiple pins form on agar, has one strain or conglomeration of strains overtaken the dish to form multiple primordia, or can multiple strains/strain groupings produce pins in the same dish?
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Re: Agar pin questions [Re: r.lutece]
#23928556 - 12/15/16 08:04 AM (7 years, 1 month ago) |
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I think I have these answers but to be honest I'm not totally sure.
Is there some one out there that has the facts on this ?
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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 21 days
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Re: Agar pin questions [Re: Th3Issu3]
#23928601 - 12/15/16 08:34 AM (7 years, 1 month ago) |
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There is lots of strains in MS - each strain has different qualities but many of them will fruit.
I think your confusing strains with genetic traits.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: Agar pin questions [Re: Mattisfat]
#23928641 - 12/15/16 08:52 AM (7 years, 1 month ago) |
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I'm specifically referring to multiple strains within a single fruit. We know that an MS bulk grow will produce fruits with multiple strains present in the tissue of a single mushroom, so my questions are based on the assumption that pins grown from MS on agar exhibit this same quality.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar pin questions [Re: Mattisfat]
#23928649 - 12/15/16 08:56 AM (7 years, 1 month ago) |
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A pin is filled with many different strains, which all have different genetic traits. Literally every tub/tray have fruits all over that are different. PE for example can have a wide array of different looking fruits, all with different strains combined to make each mushroom have a different phenotype. Even agar has different looking fruits on the dish, so the answer is yes they're different, and you're still gambling on fruiting performance.
If you take a clone and isolate the sectors, you have reduced the chance you'll see the same desirable traits because a fruit body was formed like that because of multiple strains making it up. Some might not even be able to fruit, but still can contribute to the makeup of it. < if you read the beginning he starts off with PE fruits, and they have strains in them that don't fruit at all. I bet if he did no isolating and put that clones genetic/strain makeup on 10 tubs, he'd have seen way more yield.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Thank you, Mad! Perfect answers!
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Jabensis
Daddy



Registered: 02/14/07
Posts: 541
Loc: Copperhead Road
Last seen: 3 years, 4 months
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Re: Agar pin questions [Re: r.lutece]
#23928802 - 12/15/16 10:10 AM (7 years, 1 month ago) |
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I once took tissue from One fruit body to agar then took three wedges from the radiating beautifully rhyzomorphic mycelium to grain. Those grain masters were used to generate spawn used to produce three different subs... Each one using the genes "isolated" in those wedges. I say "isolated" because they were far from isolations... Merely isolating a group of sectors as they grew out on the agar.
Those three subs, though they came from one fruit body produced three totally different fruits from each other AND the parent fungi. One produced small squat fungi but with a dense pinset, another produced tall darker fungi with only about half the density of pins... The third "isolate" produced very few fruits and were slightly different in appearance from their brothers.
What ended up being a nice experiment was carried out im identical tubs, using identical amounts of sun while being exposed to identical environmental factors... About as scientific as I could accidentally hope for.
Anyway, goes to show how different genes get expressed.
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