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OfflineDabrit
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Will the condensation on the lids of my Petri dishes eventually dissipate??
    #23925599 - 12/14/16 11:09 AM (7 years, 1 month ago)

Hey fellow shrooms folk,

So I poured my first set of 5 petris last night and inoculated with a PE syringe. 2 out of the 5 have heavy condensation on the lids will this eventually dissipate so that I can see what's going on??

I wished I would have done a little more agar pouring research prior as I simply could have just put hot water in an empty Petri dish or cup and stacked that on top of the stack to avoid this issue.

Lesson learned !


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Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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InvisiblePirateSwazey
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: Dabrit]
    #23925638 - 12/14/16 11:19 AM (7 years, 1 month ago)

The condensation you have probably won't go away.


To avoid condensation:

Stack petris when you pour them - condensation will rise to the top plate.

Where you store them, maintain a constant temperature.

If you have issues with that, a small insulated lunchbox works great.

:goodluck:


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OfflineGreg
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: PirateSwazey]
    #23925651 - 12/14/16 11:24 AM (7 years, 1 month ago)

I have to disagree with Pirate, the condensation always goes away for me after a few days.
I think it either absorbs into the agar or evaporates through the vents/parafilm on the sides of the dish.

I've seriously never had a dish stay fogged up for more than a week.
Even when there were sizable droplets of water it still went away.


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Invisibler.lutece
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: Greg]
    #23925668 - 12/14/16 11:30 AM (7 years, 1 month ago)

If you keep them in a warm environment (~100F) until they have set, you will see less condensation. It's caused by the differential between the internal agar plate temperature (~120-130F) and the external environment (~65-75F). By reducing this differential, you reduce condensation.

Try letting them cool in stacks, letting them cool in an insulated lunchbox, etc.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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InvisiblePirateSwazey
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: Greg]
    #23925703 - 12/14/16 11:42 AM (7 years, 1 month ago)

It may or may not go away for him though. You are able to equalize the temp. I said that because homeboy is in Canada and it is cold as fuck up there right now lol.

When I first started doing this I always had condensation issues that wouldn't go away because I was a younger, less financially endowed version on myself and couldn't afford to run the heat in the winter... Then I discovered the lunch box  :wink:


--------------------
 

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OfflineDabrit
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: PirateSwazey]
    #23925721 - 12/14/16 11:50 AM (7 years, 1 month ago)

Quote:

PirateSwazey said:
It may or may not go away for him though. You are able to equalize the temp. I said that because homeboy is in Canada and it is cold as fuck up there right now lol.

When I first started doing this I always had condensation issues that wouldn't go away because I was a younger, less financially endowed version on myself and couldn't afford to run the heat in the winter... Then I discovered the lunch box  :wink:




Although I do live in Canada where I live doesn't get too cold really plus the room I'm doing this in is heated. I'm pretty sure the hot cup of water trick on top of the stack should help me next go around. I do not have a furnace so it's just electric heat which shouldn't create drafts when working with the SAB.

Getting my next set of petris done this morning for my GT syringe fingers crossed the hot water trick helps me this go around.

On a side note had an embarrassing night last night, made my agar prepped the SAB then as I'm pulling off the cap off the needle for my syringe I stabbed myself with it....my thumb was bleeding everywhere and wouldn't stop bleeding for quite some time....I'm now officially part mushroom...they call me the fungi man.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineDabrit
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: Greg]
    #23925725 - 12/14/16 11:52 AM (7 years, 1 month ago)

Quote:

Greg said:
I have to disagree with Pirate, the condensation always goes away for me after a few days.
I think it either absorbs into the agar or evaporates through the vents/parafilm on the sides of the dish.

I've seriously never had a dish stay fogged up for more than a week.
Even when there were sizable droplets of water it still went away.




Good to know. I just used saran wrap on the edges of my petri dishes this will suffice right?


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineGreg
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: PirateSwazey]
    #23925731 - 12/14/16 11:54 AM (7 years, 1 month ago)

I've never used saran wrap for petris but many people do so without issues.
I think it will be fine!

Quote:

PirateSwazey said:
It may or may not go away for him though. You are able to equalize the temp. I said that because homeboy is in Canada and it is cold as fuck up there right now lol.

When I first started doing this I always had condensation issues that wouldn't go away because I was a younger, less financially endowed version on myself and couldn't afford to run the heat in the winter... Then I discovered the lunch box  :wink:




That's a fair point, it really does depend on the environment they're stored in.
The ones I keep in my fridge actually get worse in terms of condensation.


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OfflineDabrit
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: Greg]
    #23925767 - 12/14/16 12:06 PM (7 years, 1 month ago)

Quote:

Greg said:
I've never used saran wrap for petris but many people do so without version on myself and couldn't afford to run the heat in the winter... Then I discovered the lunch box  :wink:




That's a fair point, it really does depend on the environment they're issues.
I think it will be fine!

Quote:

PirateSwazey said:
It may or may not go away for him though. You are able to equalize the temp. I said that because homeboy is in Canada and it is cold as fuck up there right now lol.

When I first started doing this I always had condensation issues that wouldn't go away because I was a younger, less financially endowed stored in.
The ones I keep in my fridge actually get worse in terms of condensation.




Right on man. Can't wait to hit the ground running for some new monotubs. Will be my first time growing using agar and my first time growing PE Variety. I've heard I'll need to be patient as they take longer to colonize and fruit. Likely my GT's will be done way before my PE's which is fine I only have a smaller sized dehydrator for now anywhoo until I've set enough aside to purchase the 9 Tray Excalibur I'll be working with my 5 tray dehydrator.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineAlCapone2k
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: Dabrit]
    #23925818 - 12/14/16 12:27 PM (7 years, 1 month ago)

When I pour my petries in 10-stacks the first or second one from above will also have some condensation.

At the beginning I put a metal cup of hot water on the stack after pouring, now I don't care anymore.

I wrap the petris directly uninoculated in saran wrap or parafilm and put them afterwards in a ziploc bag and store them at room temperatur. After two or three deays the condensations disappears.

as I said it happens with parafilm and saran wrap. It just disappears.


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OfflineTiamo
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: AlCapone2k]
    #23925900 - 12/14/16 12:57 PM (7 years, 1 month ago)

In labs it is standard practice to label the bottom rather than the lid with the species, date and initials. Then you inoculate it, wrap it, and store it upside down in an incubator unstacked. The condensation should then be absorbed by the agar. If you store at room temperature it takes a bit longer.

You store upside down because mycelium produces heat when it grows producing a temperature differential and fogging up the dish. This effect is much less noticeable when you store upside down.


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Edited by Tiamo (12/14/16 12:59 PM)


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OfflineDabrit
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Re: Will the condensation on the lids of my Petri dishes eventually dissipate?? [Re: Tiamo]
    #23926166 - 12/14/16 01:59 PM (7 years, 1 month ago)

Quote:

Tiamo said:
In labs it is standard practice to label the bottom rather than the lid with the species, date and initials. Then you inoculate it, wrap it, and store it upside down in an incubator unstacked. The condensation should then be absorbed by the agar. If you store at room temperature it takes a bit longer.

You store upside down because mycelium produces heat when it grows producing a temperature differential and fogging up the dish. This effect is much less noticeable when you store upside down.




I saw this heavily debated by RR in another thread really it seems you can do it either way .


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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