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InvisibleIntelligentxfruit2
I'm a teapot


Registered: 12/02/16
Posts: 50
Loc: In a tree somewhere
Re: Is RGS more contamination resistant than ... ??? [Re: KauaiOrca]
    #23925034 - 12/14/16 05:54 AM (7 years, 1 month ago)

I've never actually done controlled experiments on spawn, only with substrates. I wish I kept better records to try and look back but theres no way.

Corn is less nutritious so I'm sure you're right. I think I might start a log here soon tracking yeild weights of different grains
Not that it hasn't been done but it'll give me a reason to change things up


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
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Re: Is RGS more contamination resistant than ... ??? [Re: tump]
    #23925066 - 12/14/16 06:21 AM (7 years, 1 month ago)

Quote:

tump said:
Never even used corn for bottle tek pasty only wheat, oats, flours. Ive tried all the grains or seeds much earlier then your tek. Straw is a good food source and your bottles are great. But are you really saying that if corn is 5.5 cents a lb and that oats are 14 cents a lb that your Yeild in bulk would be 254% better?





Tump what I'm saying is that I simply don't believe any of the gibberish you spout. Show me a grow. Period. If I thought you could even produce a yield this conversation might go somewhere. But if a person can't even achieve 100% BE with any grain, why debate the merits of one over the other.

End of day is cracked corn = bacteria. Corn = far less surface area by volume and less mycelium, less nock points, and less real nutrition.


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OfflineAK1000
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Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
Re: Is RGS more contamination resistant than ... ??? [Re: KauaiOrca]
    #23925271 - 12/14/16 08:54 AM (7 years, 1 month ago)

Quote:

KauaiOrca said:
I've found that since I switched to RGS as my grain of choice, I never get contamination problems anymore.  Don't think I'm being more cautious with sterile precautions and, in fact, I may be getting more lax in some ways because of how little a problem it has become.

I'm curious, does anyone know if RGS or any other grain has a tendency to be less likely to contaminate?    Or are they all about the same?





I haven't seen a difference between bottles that were purely RGS and bottles that were a mix of wheat/oats/wheatbran/coffee/gypsum/verm/coir (a la coir based bottle tek)... It's nearly 100% (if not 100%) success rate if I'm not an idiot during the inoculation process. I PC them at 15psi for at least 2 hours... sometimes 2.5 hours cause I'm high, lol.


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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InvisibleKauaiOrca
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Registered: 08/12/08
Posts: 3,131
Re: Is RGS more contamination resistant than ... ??? [Re: AK1000]
    #23925320 - 12/14/16 09:19 AM (7 years, 1 month ago)

Amazing to me how many threads ultimately end up in the "my grow's bigger, better and more bad-ass than yours is and I can prove it" zone. 
Quote:

AK1000 said:
Quote:

KauaiOrca said:
I've found that since I switched to RGS as my grain of choice, I never get contamination problems anymore.  Don't think I'm being more cautious with sterile precautions and, in fact, I may be getting more lax in some ways because of how little a problem it has become.

I'm curious, does anyone know if RGS or any other grain has a tendency to be less likely to contaminate?    Or are they all about the same?





I haven't seen a difference between bottles that were purely RGS and bottles that were a mix of wheat/oats/wheatbran/coffee/gypsum/verm/coir (a la coir based bottle tek)... It's nearly 100% (if not 100%) success rate if I'm not an idiot during the inoculation process. I PC them at 15psi for at least 2 hours... sometimes 2.5 hours cause I'm high, lol.




WOW!  I rarely PC grain jars for longer than 45 minutes.  If I'm doing a big bulky grain bag, maybe 60-70 minutes tops. 

I do, however rolling boil for a solid 30 minutes and steep them for another thirty before I drain and load the jars.  I'm not sure how much that kills undesirables, but I would suspect it helps somewhat.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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InvisiblePastywhyteMDiscord
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Re: Is RGS more contamination resistant than ... ??? [Re: KauaiOrca]
    #23925417 - 12/14/16 10:01 AM (7 years, 1 month ago)

Quote:

KauaiOrca said:
Amazing to me how many threads ultimately end up in the "my grow's bigger, better and more bad-ass than yours is and I can prove it" zone.




That's because people make all kinds of claims with no basis to do so. Tump hasn't posted a successful grow but has posted lots of fail. He also claims he has a US government grant to grow cubes. If you want to believe his line of crap go ahead. I'm more concerned about the people who lurk who might consider his line of BS.


Peer review is everything in science. Prove your claims.

Also 45 min is your PC time? No wonder your contam issues seemed to disappear with RGS.


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InvisibleMad Season
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Re: Is RGS more contamination resistant than ... ??? [Re: Pastywhyte]
    #23925444 - 12/14/16 10:16 AM (7 years, 1 month ago)



Lol pulled right from TMC. I do minimum 2 hours on every grain.


--------------------
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OfflineAK1000
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Registered: 06/06/16
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Re: Is RGS more contamination resistant than ... ??? [Re: KauaiOrca]
    #23925719 - 12/14/16 11:50 AM (7 years, 1 month ago)

Quote:

KauaiOrca said:
WOW!  I rarely PC grain jars for longer than 45 minutes.  If I'm doing a big bulky grain bag, maybe 60-70 minutes tops. 




Dude... I'm guessing your RGS is doing better because it's such a smaller seed that the 45 minutes was enough to sterilize it... but other grains, like oats or whatever which are slightly larger... everywhere I've read says it should be done for 90-120 minutes, more the merrier. I let it slide to at least 2 hours.


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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InvisibleKauaiOrca
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Registered: 08/12/08
Posts: 3,131
Re: Is RGS more contamination resistant than ... ??? [Re: AK1000]
    #23926312 - 12/14/16 02:43 PM (7 years, 1 month ago)

Quote:

AK1000 said:

Dude... I'm guessing your RGS is doing better because it's such a smaller seed that the 45 minutes was enough to sterilize it... but other grains, like oats or whatever which are slightly larger... everywhere I've read says it should be done for 90-120 minutes, more the merrier. I let it slide to at least 2 hours.




The only three grains I've used are WBS, Brown Rice and RGS.  And I used wheat berries for Schlerotia a couple of times ... I've never really had a lot of contamination problems with grain, but I dropped my PC times primarily because I moved to jars opposed to grain bags and read in one of the teks that it didn't take as long.  The contam problems I did have I think probably traced back to an LC that somehow got infected, but other than that, not that much.  I have never gone substrate>bulk though as never had need for that level of production.  It's mind boggling the level of production some of the growers take it to.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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InvisiblePastywhyteMDiscord
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Re: Is RGS more contamination resistant than ... ??? [Re: KauaiOrca]
    #23926421 - 12/14/16 03:03 PM (7 years, 1 month ago)

If you never spawned to bulk that why you haven't had issues with cereal grains with such short cycles. Rice has low to no endospore populations and millets small size is a bit more forgiving. But the real reason is that the unbroken colony means that bacteria harbored in it will not be able to become a mold vector. Cased grain methods like V tek, where a shake later on in the expansion is not incorporated, are very forgiving of poor sterilization logs. But that same grain used as spawn will often result in mold when spawned in open air.


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InvisibleKauaiOrca
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Registered: 08/12/08
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Re: Is RGS more contamination resistant than ... ??? [Re: Pastywhyte]
    #23926461 - 12/14/16 03:15 PM (7 years, 1 month ago)

Quote:

Pastywhyte said:
If you never spawned to bulk that why you haven't had issues with cereal grains with such short cycles. Rice has low to no endospore populations and millets small size is a bit more forgiving. But the real reason is that the unbroken colony means that bacteria harbored in it will not be able to become a mold vector. Cased grain methods like V tek, where a shake later on in the expansion is not incorporated, are very forgiving of poor sterilization logs. But that same grain used as spawn will often result in mold when spawned in open air.




Pasty - Really appreciate all your helpful posts ... You are an incredible wealth of knowledge. 

I tried the "clone water" (syringe into a stem then suck it back out) idea and it's got a nice healthy looking culture going that I'm going to G2G in probably 3-4 days.  I'm going to try it with three separate stems ... the short and fat ones are the best ... and see what happens.  So far, doesn't look like contams have surfaced, but too early to tell.  The initial growth was good and fairly fast, though.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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InvisibleIntelligentxfruit2
I'm a teapot


Registered: 12/02/16
Posts: 50
Loc: In a tree somewhere
Re: Is RGS more contamination resistant than ... ??? [Re: KauaiOrca]
    #23928077 - 12/14/16 11:47 PM (7 years, 1 month ago)

Yeah pasty killing it as always, one day man, one day.


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OfflineThe_breadsticks
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Registered: 09/23/16
Posts: 220
Last seen: 3 years, 6 days
Re: Is RGS more contamination resistant than ... ??? [Re: Pastywhyte]
    #23929644 - 12/15/16 02:46 PM (7 years, 1 month ago)

Quote:

Pastywhyte said:
Quote:

tump said:
Never even used corn for bottle tek pasty only wheat, oats, flours. Ive tried all the grains or seeds much earlier then your tek. Straw is a good food source and your bottles are great. But are you really saying that if corn is 5.5 cents a lb and that oats are 14 cents a lb that your Yeild in bulk would be 254% better?





Tump what I'm saying is that I simply don't believe any of the gibberish you spout. Show me a grow. Period. If I thought you could even produce a yield this conversation might go somewhere. But if a person can't even achieve 100% BE with any grain, why debate the merits of one over the other.

End of day is cracked corn = bacteria. Corn = far less surface area by volume and less mycelium, less nock points, and less real nutrition.





Hey pasty, what's BE?

BTW, thank you for all the help and info youve posted.


--------------------
FREE TUMP


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OfflineAK1000
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Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
Re: Is RGS more contamination resistant than ... ??? [Re: The_breadsticks]
    #23929714 - 12/15/16 03:06 PM (7 years, 1 month ago)

Quote:

The_breadsticks said:
Hey pasty, what's BE?






Biological Efficiency =        Yield of Fresh Mushrooms
                            ________________________________________  x 100
                             
                                Total weight of dry substrate used


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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Shop: Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom


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