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Offlinemoment467
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Sterile syringe protocol
    #23923423 - 12/13/16 05:33 PM (7 years, 1 month ago)

What are the steps to make a completely sterile syringe for microscopic purposes?


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Offlinethe collector
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Re: Sterile syringe protocol [Re: moment467]
    #23923513 - 12/13/16 05:55 PM (7 years, 1 month ago)

willy mico has alot of good stuff for newbies.



enjoy, this method has worked for me over my time working with spores.


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Offlinemoment467
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Re: Sterile syringe protocol [Re: the collector]
    #23923646 - 12/13/16 06:08 PM (7 years, 1 month ago)

Oh cool!. The shitaake are a really good tasty mushroom!


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InvisibleKauaiOrca
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Re: Sterile syringe protocol [Re: moment467]
    #23923756 - 12/13/16 06:33 PM (7 years, 1 month ago)

I just put 6-7 of em in a mason jar and pressure cook them for 20 mins at 15psi.  When they cool, I tighten the lid and use em as I need em.  They stay sterile indefinitely like that.


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"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

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Offlinekrypto2000
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Re: Sterile syringe protocol [Re: moment467]
    #23923789 - 12/13/16 06:45 PM (7 years, 1 month ago)

I would not flame sterilize in a glove box that you wipe down with alcohol, that could go badly. He also seemed to be hovering over top of all of his stuff and in general had quite a few bad practices, be mindful not to do that. Not that the print would have been sterile to begin with, but there's no way that syringe wasn't contaminated, it just wasn't contaminated enough.


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OnlineLogicaL ChaosM
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Re: Sterile syringe protocol [Re: krypto2000]
    #23923983 - 12/13/16 07:36 PM (7 years, 1 month ago)

boil water, suck up and spit out water 9 times into sink. On the 10th time, leave the water inside.

mission accomplished :yesnod:


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"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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Offlinekrypto2000
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Re: Sterile syringe protocol [Re: LogicaL Chaos] * 1
    #23924090 - 12/13/16 08:01 PM (7 years, 1 month ago)

I sterilize my syringes in a jar and I sterilize some water in a LC jar (a jar with an injection and airport). I then take them in front of the flow hood and suck up sterile water. If you don't have a flow hood a SAB is good. If the syringes are dirty I do what Logical Chaos suggested to clean them, but then I go the extra step to be sure.


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Offlinetump
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Re: Sterile syringe protocol [Re: krypto2000]
    #23924654 - 12/13/16 11:37 PM (7 years, 1 month ago)

Lazy bastard tek. Then dilute in 500 ml of sterile water . suck back up in


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