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Offlinekrypto2000
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Peracetic acid strength and exposure time for effective sterilization of tissue?
    #23923157 - 12/13/16 03:49 PM (7 years, 1 month ago)

I'm interested in using peracetic acid for tissue sterilization but I can only seem to find guidelines for equipment sterilization which is often done for hours and using PAA in its gaseous phase. Does anyone have experience with this or have a reference I could review? Thanks.


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OfflineShu
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Registered: 11/20/11
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Re: Peracetic acid strength and exposure time for effective sterilization of tissue? [Re: krypto2000]
    #23923848 - 12/13/16 07:01 PM (7 years, 1 month ago)

So you want to dip your fungus in PAA?


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Offlinekrypto2000
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Re: Peracetic acid strength and exposure time for effective sterilization of tissue? [Re: Shu]
    #23923925 - 12/13/16 07:23 PM (7 years, 1 month ago)

Exactly.


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OfflineShu
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Re: Peracetic acid strength and exposure time for effective sterilization of tissue? [Re: krypto2000]
    #23924203 - 12/13/16 08:37 PM (7 years, 1 month ago)

When I was cloning rose cuttings (meristem tissue explants) I tried PAA among other things and it worked okay. 30 seconds to 2 minutes minutes was all the plants could handle and still root. Another sterilant worked better (e.g. mercuric chloride) but I wouldn't use it on edibles. The PAA I used was 1% in 5% H2O2.


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