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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: FriendOfTheDevil70]
    #24959088 - 02/01/18 08:25 AM (6 years, 3 months ago)

1. If you want. Doesn't matter
2. I toss it anyway.

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InvisibleZero Nowhere
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta]
    #24960323 - 02/01/18 06:49 PM (6 years, 3 months ago)

Is brf agar an acceptable replacement for Mea  when culturing or cloning?

Edited by Zero Nowhere (02/01/18 06:50 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: Zero Nowhere]
    #24960460 - 02/01/18 07:31 PM (6 years, 3 months ago)

Of course... Mycelium grows on it

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InvisibleZero Nowhere
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta]
    #24960517 - 02/01/18 07:59 PM (6 years, 3 months ago)

Ok.  Thanks bod.  I'm just getting started with agar.  I'm glad I don't have to order mea when I have so much brf.i didn't realize it was that simple.

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: Zero Nowhere]
    #24960520 - 02/01/18 08:00 PM (6 years, 3 months ago)

any agar recipe will work unless someone says hey this one didn't work

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InvisibleZero Nowhere
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta]
    #24960528 - 02/01/18 08:07 PM (6 years, 3 months ago)

I really like the idea of the oat steep agar.  No weighing and mixing dry ingredients.  Just the steep and agar.  Could you point me toward an oat steep to agar ratio to get started?  Thanks again Bod.

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: Zero Nowhere]
    #24960529 - 02/01/18 08:09 PM (6 years, 3 months ago)

if you make oats like in my oat tek you can use the "oat water" as is or diluted with some water.

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InvisibleZero Nowhere
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta]
    #24960551 - 02/01/18 08:19 PM (6 years, 3 months ago)

Ok.  So like 400ml oat steep and 10 grams of agar powder? Sorry bod I'm sure you're a busy man.  I'll leave you be after this. Thanks again.

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: Zero Nowhere]
    #24960581 - 02/01/18 08:32 PM (6 years, 3 months ago)

2% agar is good starting point. that means 8grams in 400mL since 400mL of water is 400grams

or you could say 2% w/v when using grams since the metric equivalency of water 1g=1ml=1cm3

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InvisibleZero Nowhere
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta]
    #24960583 - 02/01/18 08:34 PM (6 years, 3 months ago)

Thanks Bod.

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InvisibleBumholio
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Re: Bod's BRF AGAR how to and "test" results [Re: Zero Nowhere]
    #25658714 - 12/05/18 09:57 AM (5 years, 5 months ago)

Ima bump this here thread because I've found it very helpful and I'm sure others will too. I've been running in to problems with my agar recently and tbh since I've started I've just followed a 2% recipe and shits been working etc etc.

Lately though I've been struggling to get good growth on my plates using GSA so reading this thread has clued me in TF.

I've new plates arriving tomorrow and cant wait to fuck about with different ratios and see what results I get.

Also this is thread is a great read:kittylaugh:


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

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Invisiblewildlifesc
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta]
    #27684745 - 03/06/22 07:52 AM (2 years, 2 months ago)

Just tried this last night using a no-pour method.  Sterilized in small mason containers.  There is a lot of sediment at the bottom, not sure if all the BRF is in the sediments and if there is any nutrient left at the surface of the agar for the mycelium.  We'll see...

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: wildlifesc]
    #27684895 - 03/06/22 10:40 AM (2 years, 2 months ago)

It'll be fine

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Offlinenakadash1
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Re: Bod's BRF AGAR how to and "test" results [Re: Violet]
    #28193301 - 02/18/23 03:49 PM (1 year, 3 months ago)

Hey, thanks for sharing your findings on BRF agar! I want to try this as well, and I was thinking of using higher quantities as you recommended but filtering it for clarity, until I read a post above where somebody did side by side comparisons and had poor results with the filtered batch.

Do you happen to know if anything in BRF actually dissolves  in water, or is it a suspension? I can't argue against the efficacy of your results, but I'm wondering if a lesser percentage works because 100% of the BRF makes its way to the plate, versus using a larger amount of BRF and discarding possibly the higher particulate sized portion of the flour.

I suppose what I want -- a high % of BRF while maintaining high clarity  might simply be impossible.


--------------------
#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold

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OfflineImJonas
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta]
    #28722390 - 04/01/24 12:09 PM (1 month, 27 days ago)

I know this is old, but reading through the entire thread I still had questions. So I ran some agar as bod described and as violet described (kind of messed that one up, but whatever).

Results:

bod's had better growth but did have more sediment.

Violet's was more clear, but had thin translucent growth.  Violet definitely is working with different ingredients, because the method just was not reproducible by me. It took way longer than it should have for the sediment to form.

Both were made and PC'd at the same time.
Same cultures transferred to each plate, at the same time and stored under similar conditions.

Preparation, bod method.
6g brf (ground in a coffee grinder)
9g A-A
Dumped into media bottle.
Topped to 350ml with boiling water.
Add food coloring.
Stir.
Cap tightly, loosed 1/8 turn.
Into the PC.

Preparation, Violet method
20g brf (ground in a coffee grinder)
Boil in 750ml of water for 20min
Place in a quart mason jar.
After 10min Pour off super goopy liquid and leave heavy sediment.
Allow to settle for 9 hours.
This finally separated out a top and bottom layer.
Pour off top layer of 400ml.
Add 200ml water
Bring to a boil and add A-A.
Place in media bottle to 300ml and spoon into no pour jars.
Add food coloring to media bottle.
Place in PC (along with freshly prepared bod bottle)

PC 30min

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