Bod's BRF AGAR how to and "test" results - Mushroom Cultivation

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 61,818
Loc: Milky way

Re: Bod's BRF AGAR how to and "test" results

[Re: FriendOfTheDevil70]
#24959088 - 02/01/18 08:25 AM (5 years, 1 month ago)

1. If you want. Doesn't matter
2. I toss it anyway.

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Everything you need in one spot.
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5g a day keeps the 5g away

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Zero Nowhere
Beer Drinker

Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon

Re: Bod's BRF AGAR how to and "test" results

[Re: bodhisatta]
#24960323 - 02/01/18 06:49 PM (5 years, 1 month ago)

Is brf agar an acceptable replacement for Mea when culturing or cloning?

Edited by Zero Nowhere (02/01/18 06:50 PM)

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 61,818
Loc: Milky way

Re: Bod's BRF AGAR how to and "test" results

[Re: Zero Nowhere]
#24960460 - 02/01/18 07:31 PM (5 years, 1 month ago)

Of course... Mycelium grows on it

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Zero Nowhere
Beer Drinker

Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon

Re: Bod's BRF AGAR how to and "test" results

[Re: bodhisatta]
#24960517 - 02/01/18 07:59 PM (5 years, 1 month ago)

Ok. Thanks bod. I'm just getting started with agar. I'm glad I don't have to order mea when I have so much brf.i didn't realize it was that simple.

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 61,818
Loc: Milky way

Re: Bod's BRF AGAR how to and "test" results

[Re: Zero Nowhere]
#24960520 - 02/01/18 08:00 PM (5 years, 1 month ago)

any agar recipe will work unless someone says hey this one didn't work

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Everything you need in one spot.
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Zero Nowhere
Beer Drinker

Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon

Re: Bod's BRF AGAR how to and "test" results

[Re: bodhisatta]
#24960528 - 02/01/18 08:07 PM (5 years, 1 month ago)

I really like the idea of the oat steep agar. No weighing and mixing dry ingredients. Just the steep and agar. Could you point me toward an oat steep to agar ratio to get started? Thanks again Bod.

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 61,818
Loc: Milky way

Re: Bod's BRF AGAR how to and "test" results

[Re: Zero Nowhere]
#24960529 - 02/01/18 08:09 PM (5 years, 1 month ago)

if you make oats like in my oat tek you can use the "oat water" as is or diluted with some water.

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Everything you need in one spot.
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Zero Nowhere
Beer Drinker

Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon

Re: Bod's BRF AGAR how to and "test" results

[Re: bodhisatta]
#24960551 - 02/01/18 08:19 PM (5 years, 1 month ago)

Ok. So like 400ml oat steep and 10 grams of agar powder? Sorry bod I'm sure you're a busy man. I'll leave you be after this. Thanks again.

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 61,818
Loc: Milky way

Re: Bod's BRF AGAR how to and "test" results

[Re: Zero Nowhere]
#24960581 - 02/01/18 08:32 PM (5 years, 1 month ago)

2% agar is good starting point. that means 8grams in 400mL since 400mL of water is 400grams

or you could say 2% w/v when using grams since the metric equivalency of water 1g=1ml=1cm³

--------------------
Everything you need in one spot.
My Q&A thread. Ask me questions here rather than in Private MessageBod's chat thread
5g a day keeps the 5g away

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Zero Nowhere
Beer Drinker

Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon

Re: Bod's BRF AGAR how to and "test" results

[Re: bodhisatta]
#24960583 - 02/01/18 08:34 PM (5 years, 1 month ago)

Thanks Bod.

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Bumholio
What's the craic

Registered: 07/23/18
Posts: 4,267
Loc: Shroomsville

Re: Bod's BRF AGAR how to and "test" results

[Re: Zero Nowhere]
#25658714 - 12/05/18 09:57 AM (4 years, 3 months ago)

Ima bump this here thread because I've found it very helpful and I'm sure others will too. I've been running in to problems with my agar recently and tbh since I've started I've just followed a 2% recipe and shits been working etc etc.

Lately though I've been struggling to get good growth on my plates using GSA so reading this thread has clued me in TF.

I've new plates arriving tomorrow and cant wait to fuck about with different ratios and see what results I get.

Also this is thread is a great read :kittylaugh:

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"great things may come to those who wait, but only what's left by those who hustle"

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wildlifesc
Taster

Registered: 02/05/22
Posts: 192
Loc: SC, USA

Re: Bod's BRF AGAR how to and "test" results

[Re: bodhisatta]
#27684745 - 03/06/22 07:52 AM (1 year, 15 days ago)

Just tried this last night using a no-pour method. Sterilized in small mason containers. There is a lot of sediment at the bottom, not sure if all the BRF is in the sediments and if there is any nutrient left at the surface of the agar for the mycelium. We'll see...

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 61,818
Loc: Milky way

Re: Bod's BRF AGAR how to and "test" results

[Re: wildlifesc]
#27684895 - 03/06/22 10:40 AM (1 year, 15 days ago)

It'll be fine

--------------------
Everything you need in one spot.
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nakadash1
Stranger

Registered: 11/02/22
Posts: 139
Last seen: 18 hours, 34 minutes

Re: Bod's BRF AGAR how to and "test" results

[Re: Violet]
#28193301 - 02/18/23 03:49 PM (1 month, 7 hours ago)

Hey, thanks for sharing your findings on BRF agar! I want to try this as well, and I was thinking of using higher quantities as you recommended but filtering it for clarity, until I read a post above where somebody did side by side comparisons and had poor results with the filtered batch.

Do you happen to know if anything in BRF actually dissolves in water, or is it a suspension? I can't argue against the efficacy of your results, but I'm wondering if a lesser percentage works because 100% of the BRF makes its way to the plate, versus using a larger amount of BRF and discarding possibly the higher particulate sized portion of the flour.

I suppose what I want -- a high % of BRF while maintaining high clarity might simply be impossible.

--------------------
#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold

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