Bod's BRF AGAR how to and "test" results - Mushroom Cultivation

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 61,818
Loc: Milky way

Bod's BRF AGAR how to and "test" results

15
#23922599 - 12/13/16 01:01 PM (6 years, 3 months ago)

3 strengths of BRF agar were made
The recipe is
400grams(mL) water
9g agar-agar (telephone brand)
and then
2/4/6 grams of BRF

that's
2.25% agar-agar
and 0.5/1.0/1.5 % BRF
and some food coloring to try to make the low medium and high light medium and dark. This didn't work that well so next time I'll use three different colors like green yellow and red or something

First I boil water and get a media bottle ready with small funnel put in the top

(media bottle)

I weigh out the 9g agar and the BRF then pour it in the funnel, then pour in boiling water until I reach a hair over the 400mL mark

I then stir and put the cap on then loosen the cap 1/8th turn

then into the pressure cooker without foil on the lids(just my style do it your way)

I PC the BRF agar for 25 minutes at 15 psi(but I run mine at 17-18). There is sediment I don't worry about it but I do like to PC longer than necessary because of it.

https://www.shroomery.org/forums/showflat.php/Number/21922023
that's my agar tek if you need supplemental information about how to prepare and use agar

and here's even more agar resources
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954

I got 17 of the 0.5% 18 of the 1.0% and 1.5%

I put PEU, Treasure Coast, Golden Teacher, and Pan Cyan spores to three plates of each strength for (4x3x3)=36 plates to test

the pans germinated first, with nearly equal performance across all the BRF strengths. dec-7 to dec-13, 6 days for growth

UPDATE 12/16:

Pans are obviously doing best on the strongest BRF agar.

golden teachers also just started to germinate, with the 1.5% being the winner, as the 0.5% and 1.0% haven't yet to germinate

UPDATE 12/16

GTs are doing the best on the highest strength agar as well

no signs of life from the TC and PEU yet

UPDATE 12/16
some life from the TC but no life from the PEU yet

here's another small test
all from the same donor ESS plate three wedges were taken each went to a different strength BRF agar
the 1.5% being clearly the strongest recovery.

UPDATE 12/16

clearly doing best on the 1.5%

--------------------
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My Q&A thread. Ask me questions here rather than in Private MessageBod's chat thread
5g a day keeps the 5g away

Edited by bodhisatta (12/16/16 04:19 PM)

Extras:

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