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InvisiblebodhisattaMDiscordReddit
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Bod's BRF AGAR how to and "test" results * 16
    #23922599 - 12/13/16 01:01 PM (7 years, 3 months ago)

3 strengths of BRF agar were made
The recipe is
400grams(mL) water
9g agar-agar (telephone brand)
and then
2/4/6 grams of BRF

that's
2.25% agar-agar
and 0.5/1.0/1.5 % BRF
and some food coloring to try to make the low medium and high light medium and dark. This didn't work that well so next time I'll use three different colors like green yellow and red or something

First I boil water and get a media bottle ready with small funnel put in the top

(media bottle)

I weigh out the 9g agar and the BRF then pour it in the funnel, then pour in boiling water until I reach a hair over the 400mL mark

I then stir and put the cap on then loosen the cap 1/8th turn

then into the pressure cooker without foil on the lids(just my style do it your way)

I PC the BRF agar for 25 minutes at 15 psi(but I run mine at 17-18). There is sediment I don't worry about it but I do like to PC longer than necessary because of it.

https://www.shroomery.org/forums/showflat.php/Number/21922023
that's my agar tek if you need supplemental information about how to prepare and use agar

and here's even more agar resources
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954




I got 17 of the 0.5% 18 of the 1.0% and 1.5%

I put PEU, Treasure Coast, Golden Teacher, and Pan Cyan spores to three plates of each strength for (4x3x3)=36 plates to test





the pans germinated first, with nearly equal performance across all the BRF strengths. dec-7 to dec-13, 6 days for growth

UPDATE 12/16:





Pans are obviously doing best on the strongest BRF agar.


golden teachers also just started to germinate, with the 1.5% being the winner, as the 0.5% and 1.0% haven't yet to germinate

UPDATE 12/16


GTs are doing the best on the highest strength agar as well

no signs of life from the TC and PEU yet

UPDATE 12/16
some life from the TC but no life from the PEU yet








here's another small test
all from the same donor ESS plate three wedges were taken each went to a different strength BRF agar
the 1.5% being clearly the strongest recovery.

UPDATE 12/16




clearly doing best on the 1.5%

Edited by bodhisatta (12/16/16 04:19 PM)

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InvisiblemushboyMDiscord
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta] * 2
    #23922614 - 12/13/16 01:06 PM (7 years, 3 months ago)

:takingnotes:

ive been wanting to try brf agar. thanks for the motivation :cheers:

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Re: Bod's BRF AGAR how to and "test" results [Re: mushboy] * 2
    #23922628 - 12/13/16 01:11 PM (7 years, 3 months ago)

I did that last summer with good results :thumbup:


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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: spirit_shadow] * 3
    #23922633 - 12/13/16 01:13 PM (7 years, 3 months ago)

I've used BRF agar years now but I never really made a thread or compared any of the strengths I usually use to see if there's a performance difference

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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta] * 1
    #23922655 - 12/13/16 01:18 PM (7 years, 3 months ago)

Quote:

bodhisatta said:
I've used BRF agar years now but I never really made a thread or compared any of the strengths I usually use to see if there's a performance difference



Lol yeah I only used it just cos I had so much I literally didnt know what to do with it. Good write up though :kaneclap:


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InvisibleMycolorado
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Re: Bod's BRF AGAR how to and "test" results [Re: spirit_shadow] * 1
    #23922675 - 12/13/16 01:24 PM (7 years, 3 months ago)

Sweet test!  Already seeing results too!

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: Mycolorado] * 2
    #23922680 - 12/13/16 01:25 PM (7 years, 3 months ago)

6 day germination for pans and some of the GT, not bad

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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta] * 2
    #23922693 - 12/13/16 01:30 PM (7 years, 3 months ago)

I like brf agar :grin:

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Re: Bod's BRF AGAR how to and "test" results [Re: Pastywhyte] * 1
    #23922702 - 12/13/16 01:33 PM (7 years, 3 months ago)

i can only assume using pf style brf wouldnt work.

you need flour ...'grade' brf. correct?

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: mushboy] * 1
    #23922708 - 12/13/16 01:35 PM (7 years, 3 months ago)

I just use the stuff I buy at whole foods.

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Offlineenlightenment
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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta] * 1
    #23922725 - 12/13/16 01:40 PM (7 years, 3 months ago)

:popcorn:

:threadmonitor:

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Re: Bod's BRF AGAR how to and "test" results [Re: enlightenment] * 1
    #23922752 - 12/13/16 01:48 PM (7 years, 3 months ago)

BRF agar is all I've ever used... treated me well so far.


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InvisiblebodhisattaMDiscordReddit
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Re: Bod's BRF AGAR how to and "test" results [Re: Thedenthead] * 1
    #23922764 - 12/13/16 01:55 PM (7 years, 3 months ago)

Those of you who used it what was your recipes and got any pics.

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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta] * 1
    #23922789 - 12/13/16 02:06 PM (7 years, 3 months ago)

I just made a suspension similar to the ratios  violet used in her poms write up. Then I added a bit of karo and just made agar with it.

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Re: Bod's BRF AGAR how to and "test" results [Re: bodhisatta] * 1
    #23922893 - 12/13/16 02:36 PM (7 years, 3 months ago)

Quote:

bodhisatta said:
Those of you who used it what was your recipes and got any pics.



Sadly no. But I will make some more soon just for comparison :thumbup:


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Re: Bod's BRF AGAR how to and "test" results [Re: spirit_shadow] * 1
    #23922940 - 12/13/16 02:47 PM (7 years, 3 months ago)

Im tryn this for sure


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Re: Bod's BRF AGAR how to and "test" results [Re: Nobler Hino] * 1
    #23923146 - 12/13/16 03:45 PM (7 years, 3 months ago)

Nice one bod:rockon:


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Re: Bod's BRF AGAR how to and "test" results [Re: mrmazdarx9] * 1
    #23923311 - 12/13/16 05:02 PM (7 years, 3 months ago)

:jolly:

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Re: Bod's BRF AGAR how to and "test" results [Re: germanauslander] * 1
    #23931523 - 12/16/16 04:43 AM (7 years, 3 months ago)

Nice Bod :thumbup:
I did brf agar months ago but I added 1% SITR in the recipe because someone I trusted told me so, don't remember who.
Last night I pc'd a bunch of brf plates (1,5% BRF, no SITR) and I'm going to noc them up today with Jalisco, Pans and TOC.
:holyshitbubbles:

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Re: Bod's BRF AGAR how to and "test" results [Re: Josex] * 2
    #23932912 - 12/16/16 04:21 PM (7 years, 3 months ago)

some updates.
the 1.5% is the clear winner

next time I'll probably do 6g 10g 14g or 1.5% 2.5% and 3.5%

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