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Offlinerunsfrombears
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Agar dishes ... tell me what I'm seeing
    #23921765 - 12/13/16 06:20 AM (7 years, 1 month ago)

Okay, I'm pretty new to making my own PDA dishes and I could use some help deciphering what's going on in my dishes.
I inoculated them all with a Liquid inoculant syringe I made ... I tried my best to get one drop but mostly it just sprayed onto my agar.







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Offlinemrmazdarx9
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Re: Agar dishes ... tell me what I'm seeing [Re: runsfrombears]
    #23921863 - 12/13/16 07:54 AM (7 years, 1 month ago)

Your supposed to take a transfer as soon as you see the myc growing to hopefully escape from contams that grow a little later


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OfflinePinpapa
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Re: Agar dishes ... tell me what I'm seeing [Re: mrmazdarx9]
    #23921929 - 12/13/16 08:33 AM (7 years, 1 month ago)

Take smalll pie shaped pieces from good looking edges of new growth.Transfer and repeat.Keep all pltes till they contam or get big and white. After 3-7 days most shit will show if not they are clean. Then transfer to grain,wood  or lc and multiply!Mould is the tough one to get around cause it looks like myc and outgrows everything else.They look ok, take some from the edge of one of the denser growths.


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Edited by Pinpapa (12/13/16 08:36 AM)


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InvisibleMad Season
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Re: Agar dishes ... tell me what I'm seeing [Re: Pinpapa]
    #23921988 - 12/13/16 09:05 AM (7 years, 1 month ago)

Right.. they look ok... :sad: they look awful if you asked me.

I'd probably just start over tbh. Yeah you could probably clean it up, but I'd just start over and save yourself some time. Transfer when the growths are like nickel sized.


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Offlinemrmazdarx9
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Re: Agar dishes ... tell me what I'm seeing [Re: Pinpapa]
    #23922003 - 12/13/16 09:14 AM (7 years, 1 month ago)

Quote:

Pinpapa said:
Take smalll pie shaped pieces from good looking edges of new growth.Transfer and repeat.Keep all pltes till they contam or get big and white. After 3-7 days most shit will show if not they are clean. Then transfer to grain,wood  or lc and multiply!Mould is the tough one to get around cause it looks like myc and outgrows everything else.They look ok, take some from the edge of one of the denser growths.



How do they look ok?  i was saying he left it too long now the jars are nasty


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Offlinerunsfrombears
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Re: Agar dishes ... tell me what I'm seeing [Re: mrmazdarx9]
    #23922294 - 12/13/16 11:03 AM (7 years, 1 month ago)

Relax. These have all being trashed already. I'm just looking for any explanation for why they would grow in that weird pattern.
..... I would like to learn from these failed dishes and any information involved with "why" would be appreciated.


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InvisibleMad Season
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Re: Agar dishes ... tell me what I'm seeing [Re: runsfrombears]
    #23922310 - 12/13/16 11:11 AM (7 years, 1 month ago)

Because the other shit like bacteria, or other white molds like vert in your syringe contaminated it.

You have to transfer before then to get good growths going.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinerunsfrombears
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Re: Agar dishes ... tell me what I'm seeing [Re: Mad Season]
    #23922375 - 12/13/16 11:35 AM (7 years, 1 month ago)

So there IS mold and bacteria in there?

I honestly can't tell what's going on in there.

When they are good, it's pretty obvious but ... Trying to figure out what's going on in that mess, I'm finding difficult.


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InvisibleMad Season
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Re: Agar dishes ... tell me what I'm seeing [Re: runsfrombears]
    #23922412 - 12/13/16 11:47 AM (7 years, 1 month ago)

I'm saying it could be either, I have no idea for sure without being there and watching it grow/using a scope.

https://www.shroomery.org/forums/showflat.php/Number/22020260
This thread might help.

https://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1
This is a great one too


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinerunsfrombears
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Re: Agar dishes ... tell me what I'm seeing [Re: Mad Season]
    #23922602 - 12/13/16 01:02 PM (7 years, 1 month ago)

Oh, cool. Thanks.


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