|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: AndyHinton]
#24016886 - 01/17/17 09:37 AM (7 years, 13 days ago) |
|
|
3 more one step clone water "pasty-pucks" ready for action ... G2G to other jars or agar or both.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (01/17/17 09:48 AM)
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24016932 - 01/17/17 09:57 AM (7 years, 13 days ago) |
|
|
Quote:
KauaiOrca said: 3 more one step clone water "pasty-pucks" ready for action ... G2G to other jars or agar or both.

What grain is that? Those look like hella bacteria
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Anyone Tried Cloning This Way??? [Re: Pastywhyte]
#24016935 - 01/17/17 09:59 AM (7 years, 13 days ago) |
|
|
Yea those ain't healthy.
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: Pastywhyte]
#24016990 - 01/17/17 10:22 AM (7 years, 13 days ago) |
|
|
Quote:
Pastywhyte said:
Quote:
KauaiOrca said: 3 more one step clone water "pasty-pucks" ready for action ... G2G to other jars or agar or both.

What grain is that? Those look like hella bacteria 
RGS ... probably need another day or two to finish up ... The others were about the same and both grew out two good jars when I split em up. Might not be good for bulk, though ... I don't know. I did take some agar samples off one and didn't see any visible contam on the agar, but would take a microscope to be sure. I've done a lot of really good jars from pucks that look just like that.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (01/17/17 10:25 AM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca] 1
#24017048 - 01/17/17 10:50 AM (7 years, 13 days ago) |
|
|
Quote:
Pastywhyte said:
Quote:
KauaiOrca said:
How does mold enter the equation?
Bacteria is a vector for mold.
and those are bacterial. your idea is acceptable for your needs but i wouldnt do it. but its also a good demonstration of how easy cubes are to grow
good job with your grows. ive liked this thread. its shroomy
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: mushboy]
#24017068 - 01/17/17 10:57 AM (7 years, 13 days ago) |
|
|
Quote:
mushboy said:
Quote:
Pastywhyte said:
Quote:
KauaiOrca said:
How does mold enter the equation?
Bacteria is a vector for mold.
and those are bacterial. your idea is acceptable for your needs but i wouldnt do it. but its also a good demonstration of how easy cubes are to grow
good job with your grows. ive liked this thread. its shroomy
It might be bacterial ... I don't know ... I'll take your guys word for it as you're a lot more experienced than I am. I've found personally, from the smaller (definitely not bulk) grows that I tend to do, that whatever level of bacteria this is, has little impact on yield, if any. And, since with each harvest I'm starting over with a fresh new clone water sample, the bacteria, if it exists, never gets chance to gain any ground with generations of expansion because the new clone water sample always starts relatively clean. I used to put a lot of effort into getting a squeaky clean strong LC and just grow from that over and over, but I kinda got bored with it, to be honest. The idea of having a different clone growing each new jar became a lot more interesting so I start a new MS grow every once in awhile to refresh the genetics and then each jar from that point is off of its own individual clone water start. There's a lot of mystery to it with each jar having it's own individual personality.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (01/17/17 11:03 AM)
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24017118 - 01/17/17 11:20 AM (7 years, 13 days ago) |
|
|
I'd be curious if you guys think that the pictures from post #23978484 in this thread (page 4 on my count) look bacterial or not?
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24017123 - 01/17/17 11:22 AM (7 years, 13 days ago) |
|
|
Quote:
kenetic said: Nice! your sub does seem pretty bacterial though, based on the pressed look of the grains on the glass.....
No offence but not a fan. Like I said, no offence.
few replies later.
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: mushboy]
#24017192 - 01/17/17 11:51 AM (7 years, 13 days ago) |
|
|
Quote:
mushboy said:
Quote:
kenetic said: Nice! your sub does seem pretty bacterial though, based on the pressed look of the grains on the glass.....
No offence but not a fan. Like I said, no offence.
few replies later.
The jar in the picture (bacterial) that was cased with verm grew out really good. I can honestly say I don't think I've ever lost a jar, at least that I'm aware of, because it went bacterial ... I've lost some to mold for sure. Only really serious bacteria issue I ever had was with a print I got from someone else that had a lot of red and black stuff that grew from it, but my own prints and clones don't have bacteria that impacts the grow much.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24017243 - 01/17/17 12:13 PM (7 years, 13 days ago) |
|
|
Bacteria isn't a huge issue if the colony remains unbroken. When spawning even small amounts of bacteria can lead to mold. However in my experience bacteria will lead to a small drop in BE even in the case of an unbroken colony.
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: Pastywhyte]
#24017286 - 01/17/17 12:37 PM (7 years, 13 days ago) |
|
|
Quote:
Pastywhyte said: Bacteria isn't a huge issue if the colony remains unbroken. When spawning even small amounts of bacteria can lead to mold. However in my experience bacteria will lead to a small drop in BE even in the case of an unbroken colony.
Thanks, P. Your knowledge and advice is ALWAYS appreciated.
Good luck with your mother of all tubs grow.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24029740 - 01/22/17 06:25 AM (7 years, 8 days ago) |
|
|
Latest Clone Water Jar getting going ....
This latest jar, from clone water extraction to these pins (which showed up about 3 days ago) was exactly 31 days. Looks like it will be a pretty big first flush as this strain tends to grow big, tall fruits. The mushroom I took the clone water out of was from a MS grow ... I'm going to do clone water starters with 2-3 of the mushrooms from this flush and see how many generations it will work. Straight RGS cased with VERM.

-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (01/22/17 06:35 AM)
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24029777 - 01/22/17 07:21 AM (7 years, 8 days ago) |
|
|
Here are two jars three days after I inoculated them with a "clone water puck" which is a pastyplate type PP5 with RGS inoculated with the clone water ... it colonized then I "tiger dropped" the puck into a wide mouthed pint mason jar with about 50ml's of sterile water ...

... then shook the crap out of it then split it into these two jars, shook violently and here's what they look like 3-days later.

The puck was slow to colonize ... took 17-days. But it seems to be colonizing these jars pretty fast. Might have been that the clone water inoculation just contained a tiny amount of tissue that took a while to replicate and get going.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (01/22/17 07:24 AM)
|
ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24038550 - 01/25/17 12:31 PM (7 years, 5 days ago) |
|
|

Im loving this
I would really like to see pics of exactly where and at what angle that you make your scalpel cuts and injections on these fruits
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
Edited by ichugwindex (01/25/17 12:54 PM)
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: ichugwindex]
#24047244 - 01/28/17 06:57 PM (7 years, 2 days ago) |
|
|
First Flush from a Clone Water Jar that is just pure RGS cased with verm. This one took
-17 days to colonize the small PP5 RGS puck (longer than others) after clone water inoculation - 10 days to fully colonize a 2/3 full PP5 Quart with RGS after G2G with grain puck - 12-days to pin after being cased with verm - 7 days after first pins until this picture.

These were from clone water from an MS grow and I'll start 3 more clone water pucks from these fruits that will be clones from the first generation clone water to see if growth characteristics are similar. Most likely, the 2nd flush will be bigger than the first.
It's not as big a flush as most of the others and it came from a clone water puck that was slow to colonize. Two of the fruits were 9+ inches tall, though Probably the weakest of all the clone water jars I've done so far.
For a noob that wants a pretty simple way to grow a steady stream of mushrooms from jars with basically two ingredients ... RGS and Verm ... with no fruiting chamber needed and wants to get into cloning, this is pretty frigging easy. If you want to get into bigger bulk grows, this is not the path ... It might be, but it hasn't been tested. Certainly, though, to create a never ending nearly free (about 20 cents a jar) supply of (15-30 oz's dry per jar) them, this is a very reliable way to do it. Agar is more certain, but a lot more steps and time.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (01/28/17 11:06 PM)
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: ichugwindex]
#24047259 - 01/28/17 07:04 PM (7 years, 2 days ago) |
|
|
Quote:
ichugwindex said:

Im loving this
I would really like to see pics of exactly where and at what angle that you make your scalpel cuts and injections on these fruits
It's very simple. After picking the fruit, I take a red hot scalpel and just cut about 1/3 of an inch off the bottom into the meaty part of the bottom of the stem. Then, I insert a red hot syringe needle right into the center of the meaty bottom that has just been sliced up into the stem holding it horizontally. You can easily feel when the tip of the needle gets through the meaty thicker bottom into the hollow part of the stem. You know the center because there is, at least on my fruits, a small very white center that I just use to target the going in spot. Then I slowly squeeze ALL (about 2 ml) of the water into the hollow stem then pull the needle back until it "feels" like it's just coming back into the solid meaty part of the bottom. Then I turn the fruit so it is upright and gravity pulls the water I pushed in down toward the waiting tip of my needle that is now just above the solid part of the bottom of the stem and then I slowly try and suck back the water I pushed in. I usually get about 5-7 drops or so. Then I pull it out and quickly quickly inject this water into a small sterilized PP5 jar with a little RGS in it right through a foil top (I hate those plastic lids they come with) and tape the small hole and put it aside to colonize. Entire procedure is 15-30 seconds. Thus far, I haven't been doing this in an SAB and haven't had any of the PP5 small pucks contaminate.
I don't claim it's 100% sterile and don't know, to be totally honest, but I do know it's clean enough to consistently colonize a small RGS puck that's clean enough to G2G to a couple of quart jars that fruit consistently as good as the picture in the post above.
I suppose if I find a clone that fruits exceptionally well and I want to keep it, I'll just put some clone tissue from that harvest on agar and clean it up to prep it for a LC jar although I don't think that's really necessary given how easy and simple it is to start new starter pucks when I harvest. I just now sterilize 10 small syringes at a time in the PC with 2 ml's of water already in them so they're 100% ready for use at harvest time. I have f'd the procedure up a couple of time and it's really no big deal ... I just pick another fruit, grab another syringe and try it again. Does no harm at all to the fruits and they just go into the the dehydrator with all the rest.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (01/28/17 07:19 PM)
|
ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24051877 - 01/30/17 02:16 PM (7 years, 11 hours ago) |
|
|
I like this. I also enjoy how you are taking your prints and Just finished getting a print like this myself. Way to go man
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: ichugwindex]
#24064363 - 02/04/17 06:12 AM (6 years, 11 months ago) |
|
|
Quote:
ichugwindex said:
I like this. I also enjoy how you are taking your prints and Just finished getting a print like this myself. Way to go man
Thanks! Here's another PP5 from a clone water starter that is ready for harvest this morning in its own, self contained fruiting chamber ... no misting, fanning, drilling holes, perlite, stuffing with polyfill, micropore tape, tyvek or special lighting needed. If there's an easier or faster way to quickly and reliably clone and fruit jars after jar without agar, I haven't come across it.

Took a total of 26 days from clone water procedure until this. Clone water on to straight RGS ... cased with Verm ... bottom watered when pins showed up.
When I harvest, I'll do the same clone water procedure to a couple of them and start two new small pp5 grain pucks ... the cycle starts again.... and it always starts with what is proving to be very clean clone tissue that is fully capable of colonizing a jar and fruiting well without contaminating. If you just want a steady, never ending supply of fresh mushrooms coming in flushes of 80-125 grams (wet) with 5-10 continuous jars that can all fit literally on a 18" x 24" book case slot, this is a very easy way to do it. No liquid cultures, agar, LI, Blenders, SAB, complex growing formulas, pasteurizing, or any of that stuff.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (02/04/17 07:23 AM)
|
KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24064388 - 02/04/17 06:36 AM (6 years, 11 months ago) |
|
|
Here's a second flush getting ready to take off from a clone water jar pictured a few posts back. Looks like it will be about double the size of the first flush.

Now is the perfect time to bottom water this jar.
This is the same jar that a number of people had said looked loaded with bacterial contams.
One of the nice pluses of growing this way is that side pinning is a complete non-issue ... if they side pin... great ... if they pin from the center ... great ... makes no difference. They're always easy to harvest and side pins grow just as big as the others.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (02/04/17 07:11 AM)
|
oontribe


Registered: 01/14/15
Posts: 3,570
|
Re: Anyone Tried Cloning This Way??? [Re: KauaiOrca]
#24064456 - 02/04/17 07:13 AM (6 years, 11 months ago) |
|
|
monitoring this thread
|
|