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Invisibleflyontoast
Farming food; farming time
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Registered: 08/20/16
Posts: 258
Which culture is better to expand (petri pics)
    #23916205 - 12/11/16 12:40 PM (7 years, 1 month ago)

These are Shiitake 3782, both from same master culture. Tried to get rid of the condensation with a hot glass, this was the best I could get.

This is on Gluten-Free Flower (so much contam on other plates).


And this one is boiled Grain-Straw Water


I'm about to make another plate and a grain master. Which of these look better/healthier/more vigorous and why? Neither look at all like the master petri I received, which was fully colonized on an agar of unknown nutrients.
Thanks for the tips and help everyone, super grateful.


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:mushroom: My trade list :mushroom:
Looking for strong terrestrial fruiters for an outdoor beds experiment:
Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors.
Also, any commercially viable Pleurotus, cold or hot strains.
Thanks for the Q&A, trades, and all the posters & teachers that have come before us  :skol:


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Offlinedrake89
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Registered: 06/26/11
Posts: 4,168
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Re: Which culture is better to expand (petri pics) [Re: flyontoast]
    #23916410 - 12/11/16 01:57 PM (7 years, 1 month ago)

shouldn't make a difference, the mycelium just looks different on different media.  assuming they're both clean and the same generation.


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Offlineshroombasa
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Re: Which culture is better to expand (petri pics) [Re: flyontoast]
    #23916502 - 12/11/16 02:27 PM (7 years, 1 month ago)

Very nice!  I'd opt for the leggier petris over the denser ones.  Looks like they're moving a tad quicker.


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Invisibleflyontoast
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Registered: 08/20/16
Posts: 258
Re: Which culture is better to expand (petri pics) [Re: shroombasa]
    #23917151 - 12/11/16 05:41 PM (7 years, 1 month ago)

How would you be able to tell if a culture HAS lost vigor. Like, I can see how you would notice your own strain IS losing vigor because you work with it for months/years, but if you received a plate from someone, how are you to know if it's shitty just by looking at it on the plate? The master plate was fully colonized when I received it and was thick and thin in some places and wasn't a uniform mass.
Like I've seen on this forum both claims that aerial growth is a since of vigor and other posts saying it's a sign of senescence (and that person said over time their culture stopped with aerial growth and then became a better fruiter). I know this is complicated because "every strain looks different" but let's just stick to common Pleurotus and Lentinula as examples.
I also received a blue oyster LC, supposedly a clone of Stamets' Columbinus, but when I cloned it to a plate, it looks thin and wispy.


--------------------
:mushroom: My trade list :mushroom:
Looking for strong terrestrial fruiters for an outdoor beds experiment:
Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors.
Also, any commercially viable Pleurotus, cold or hot strains.
Thanks for the Q&A, trades, and all the posters & teachers that have come before us  :skol:


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Offlinedrake89
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Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Re: Which culture is better to expand (petri pics) [Re: flyontoast]
    #23917754 - 12/11/16 08:14 PM (7 years, 1 month ago)

the reason your grain/straw agar might be faster and lower is because of lower nutrients in the media.  you can run mycelium out on water agar, after all, for storage.


--------------------
Fiery Fungi (like us on faeboo)


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