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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Which culture is better to expand (petri pics)
#23916205 - 12/11/16 12:40 PM (7 years, 1 month ago) |
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These are Shiitake 3782, both from same master culture. Tried to get rid of the condensation with a hot glass, this was the best I could get.
 This is on Gluten-Free Flower (so much contam on other plates).

And this one is boiled Grain-Straw Water

I'm about to make another plate and a grain master. Which of these look better/healthier/more vigorous and why? Neither look at all like the master petri I received, which was fully colonized on an agar of unknown nutrients. Thanks for the tips and help everyone, super grateful.
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Re: Which culture is better to expand (petri pics) [Re: flyontoast]
#23916410 - 12/11/16 01:57 PM (7 years, 1 month ago) |
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shouldn't make a difference, the mycelium just looks different on different media. assuming they're both clean and the same generation.
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shroombasa
Sojourner



Registered: 05/02/15
Posts: 364
Loc: 'Merka
Last seen: 3 years, 3 months
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Re: Which culture is better to expand (petri pics) [Re: flyontoast]
#23916502 - 12/11/16 02:27 PM (7 years, 1 month ago) |
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Very nice! I'd opt for the leggier petris over the denser ones. Looks like they're moving a tad quicker.
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Peace and long life.
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Which culture is better to expand (petri pics) [Re: shroombasa]
#23917151 - 12/11/16 05:41 PM (7 years, 1 month ago) |
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How would you be able to tell if a culture HAS lost vigor. Like, I can see how you would notice your own strain IS losing vigor because you work with it for months/years, but if you received a plate from someone, how are you to know if it's shitty just by looking at it on the plate? The master plate was fully colonized when I received it and was thick and thin in some places and wasn't a uniform mass. Like I've seen on this forum both claims that aerial growth is a since of vigor and other posts saying it's a sign of senescence (and that person said over time their culture stopped with aerial growth and then became a better fruiter). I know this is complicated because "every strain looks different" but let's just stick to common Pleurotus and Lentinula as examples. I also received a blue oyster LC, supposedly a clone of Stamets' Columbinus, but when I cloned it to a plate, it looks thin and wispy.
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Re: Which culture is better to expand (petri pics) [Re: flyontoast]
#23917754 - 12/11/16 08:14 PM (7 years, 1 month ago) |
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the reason your grain/straw agar might be faster and lower is because of lower nutrients in the media. you can run mycelium out on water agar, after all, for storage.
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