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Ferather
Mycological



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Testing | Yeast Nutrients
#23915386 - 12/11/16 07:37 AM (7 years, 1 month ago) |
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Anyone here have any caffeine powder or tablets you can crush, It should replace sugars. Caffeine is energy attached to nitrates, and should not be bacteria prone.
In theory it should be restricted to live mycelium only.
No idea on ratio, but I think its unlimited.
Edited by Ferather (12/15/16 10:42 AM)
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Hobbyist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23915502 - 12/11/16 08:27 AM (7 years, 1 month ago) |
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That's a novel idea. I don't know if the mycelium could process that as easily as simple sugars.
Why don't you try it and let us know? Pure caffeine powder is readily available. If you're unable to acquire some I can send you some.
-------------------- Everything i say is completely hypothetical...
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Ferather
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Re: Test Request | Caffeine LC [Re: Hobbyist]
#23915566 - 12/11/16 08:44 AM (7 years, 1 month ago) |
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Here is a snippet from: Paul Stamets - How Mushrooms Can Help Save the World (figure 332). It specifically says that mycelium break down caffeine. I will look around on eBay.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23916509 - 12/11/16 02:28 PM (7 years, 1 month ago) |
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I may implement this with shitake! Dude great work again 0.0
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Marty Mycfly
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I have been wanting to do a test for a while with coffee. I like to make LI with sterile water, a blender attachment and agar dishes. I was thinking about doing a test where instead of sterilizing water to blend the agar I would sterilize fresh brewed coffee and blend the agar with that and then pour into my grain bags.
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mike-ologist
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Ive grown blue oyster mushrooms prolifically on coffee grounds, and i belieieve ive added grounds to a shitake grow once as well
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tump
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It's slow as shit on that. I made energy drink wine that had no preservatives in it. Took three months to ferment. Try lc of cubes with the same stuff it contaminate blue mold. Most pills have additives that prevent fungus spreading. Try it your way. Im interested
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keifnnugs
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Re: Test Request | Caffeine LC [Re: tump]
#23917311 - 12/11/16 06:20 PM (7 years, 1 month ago) |
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That's an interesting idea. Caffeine powder is so cheap.. I may give this a go..
How much would you think you would start with? I wonder if it would make a difference if you hydrate your grains with it
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Ferather
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Re: Test Request | Caffeine LC [Re: tump]
#23918818 - 12/12/16 07:57 AM (7 years, 1 month ago) |
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Quote:
tump said: It's slow as shit on that. I made energy drink wine that had no preservatives in it. Took three months to ferment. Try lc of cubes with the same stuff it contaminate blue mold. Most pills have additives that prevent fungus spreading. Try it your way. Im interested
Not enough micro & macro nutrients in energy drink, It's also high in sugar and only 32mg caffeine. Capsules are free from: Artificial flavours, colours & preservatives, lactose, yeast & gluten.
Yesterday I did some looking around, most capsules are 200 - 400mg. Which means I would need 8 capsules for 3g to 100g water.
Next issue, I would need to add: P, K, Mg, Zn.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23919261 - 12/12/16 11:27 AM (7 years, 1 month ago) |
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I wonder if boiling some sliced carrots will extract enough to use as base. Then I could add the caffeine and make a basic LC from that mix.
When cooking any vegetables you extract nutrients.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23919359 - 12/12/16 11:57 AM (7 years, 1 month ago) |
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You could also try a natural tea: Gauyusa,carrot, potato, ect.
Vs. Capsule: ie using supplements from gnc or some other supplement store :3
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Ferather
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You mean test water vs test water with caffeine?
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23919743 - 12/12/16 02:03 PM (7 years, 1 month ago) |
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Kinda, more natural caffien vs synthetic
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Ferather
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I made some carrot water and added yeast nutrients to boot. I think its enough to be a LC alone. So I will put some dirty spawn into it later and see what happens after a few days.
The mixture is typically carrot, honey looking, but slightly misty.
I did 20g of diced carrot to 60g of nutrient water.

1.25g of nutrients to 1000g water.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23922037 - 12/13/16 09:39 AM (7 years, 1 month ago) |
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23922070 - 12/13/16 09:53 AM (7 years, 1 month ago) |
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Cool stuff, how much did the willow nutrient cost, where did you get it?
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Ferather
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Quote:
mike-ologist said: Cool stuff, how much did the Wilko nutrient cost, where did you get it?
£1 from a local general shop, home beer making section.
Here is the assembly, lets see what happens.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23922330 - 12/13/16 11:17 AM (7 years, 1 month ago) |
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Sweet dude! Ill have to look into it
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Ferather
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Well it works, Yeast nutrients, something to add to your mushroom toolbox. Mycelium growing out in 24 hours, including debris from swirling.
Carrots + YN, enough energy and nutrients to grow.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23925936 - 12/14/16 01:07 PM (7 years, 1 month ago) |
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Awesome dude!
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Ferather
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There is also lots of surface growth, and a clear ring.

No signs of bacteria so far.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23928492 - 12/15/16 07:16 AM (7 years, 1 month ago) |
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The mock test has given me results, some bacteria or yeast which is expected (dirty spawn). Lots of quick growing surface growth, which I thought was scum until I swirled it.
The surface growth snapped in two when I tilted the jar.

Bubbles due to bacteria or yeast.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23928523 - 12/15/16 07:41 AM (7 years, 1 month ago) |
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I had tried a shitake liquid culture in some wood juice before, i obtained simmilar results.
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Ferather
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I've decided to continue testing Yeast Nutrients in various scenarios. I have changed the name of the original thread, From LC tests.
Feel free post your experiments here, better sharing.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23929060 - 12/15/16 11:42 AM (7 years, 1 month ago) |
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Here is something interesting, I have a cheap vendor bag of Pink oyster, rife with crap. I went to throw it out, but then decided to put it in the fridge at 2°C, regardless.
Two months later, it's still alive and active, but very slow. There is also metabolites for obvious reasons.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23929468 - 12/15/16 01:47 PM (7 years, 1 month ago) |
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Further thought, the carrot + YN LC was roughly 0.5% sugar and starch, 0.5% misc energy. Mycelium need C-H-O to produce Glycogen, Water is H2O so I need carbon only.
Yeast nutrients: magnesium-carbonate. Lime: calcium-carbonate.
The mycelium should reassemble said materials.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23931722 - 12/16/16 08:40 AM (7 years, 1 month ago) |
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The yeast nutrients and lime mix makes sediment and tints blue. I have a "possible" reaction in less than 40 minutes.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23931734 - 12/16/16 08:45 AM (7 years, 1 month ago) |
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Crazy!
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0yster
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Re: Test Request | Caffeine LC *DELETED* [Re: mike-ologist]
#23931757 - 12/16/16 08:59 AM (7 years, 1 month ago) |
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Post deleted by 0yster
Reason for deletion: -
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Ferather
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Re: Test Request | Caffeine LC [Re: 0yster]
#23934742 - 12/17/16 08:04 AM (7 years, 1 month ago) |
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The sample sank, there is possible surface growth.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23937142 - 12/18/16 02:54 AM (7 years, 1 month ago) |
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Very difficult to see without light, but its expanding.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23937935 - 12/18/16 12:37 PM (7 years, 1 month ago) |
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Test confirmed, mycelium does reassemble sources.

From debris, not the main sample.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23937940 - 12/18/16 12:41 PM (7 years, 1 month ago) |
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I also confirm the use of yeast nutrients and the improved efficiency it adds.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23937949 - 12/18/16 12:42 PM (7 years, 1 month ago) |
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Interesting!
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Ferather
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I don't the time nor resources to test every application. However you should be able to add it to current recipe's. Such as an additive to agar or liquid cultures, or adding it to water for substrates, casing or misting.
If you find uses for it please post them here to share, thanks.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23938019 - 12/18/16 01:16 PM (7 years, 1 month ago) |
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Ill have to try soaking wood chips in this when i set my shitake up
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Ferather
Mycological



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Here's today, no bacteria, no yeast and no bubbles.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23942914 - 12/20/16 10:07 AM (7 years, 1 month ago) |
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Here is the lime and yeast nutrients water after a swirl.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23949602 - 12/22/16 04:25 PM (7 years, 1 month ago) |
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I was just looking at Summer oyster on Shroomery. Right at the bottom confirms addition of nutrients.
"Okwujiako found that the vitamin thiamine was critical for growth and fruit body development in P. pulmonarius. By simply adding yeast extract to the medium, vitamins essential for enhanced production are provided."
Yeast nutrients also supplies sulfur, which increases protein production, and promotes larger caps.
Much better than Marmite
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Ferather]
#23949641 - 12/22/16 04:50 PM (7 years, 1 month ago) |
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Nice! Maybe there should be yeast strain experimentation, to see wich helps the most!
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23949670 - 12/22/16 05:07 PM (7 years, 1 month ago) |
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I have decided to revive my old WL-Tek as a substrate casing mix. It produces intense coloured fruits, and much larger caps.
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Forrester
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Re: Test Request | Caffeine LC [Re: Ferather]
#23949835 - 12/22/16 06:35 PM (7 years, 1 month ago) |
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Quote:
Ferather said: Here is something interesting, I have a cheap vendor bag of Pink oyster, rife with crap. I went to throw it out, but then decided to put it in the fridge at 2°C, regardless.
Two months later, it's still alive and active, but very slow. There is also metabolites for obvious reasons.
Pink oyster, alive after 2 months at 2°C, really? Are you sure? That species usually dies if refrigerated. If not, it's a good strain you have there, I'd try to clean it up and save it! And while you're at it, send me a copy
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Ferather
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Re: Test Request | Caffeine LC [Re: Forrester]
#23949963 - 12/22/16 07:35 PM (7 years, 1 month ago) |
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The slime and smell made me throw it out about a week ago. Never got around to cleaning it. I got the infected spawn from a vendor, So I can get more another time.
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Forrester
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Re: Test Request | Caffeine LC [Re: Ferather]
#23950082 - 12/22/16 08:23 PM (7 years, 1 month ago) |
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Yeah, I almost guarantee you that slime and smell was because the culture was dead and the contam's had taken over. Like you said, it was probably already contaminated to begin with, but just so you know, do some research before refrigerating your next pink oyster culture, it may not be the best idea.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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mike-ologist
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Re: Test Request | Caffeine LC [Re: Forrester]
#23950179 - 12/22/16 08:58 PM (7 years, 1 month ago) |
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I dont know if theres a different rule for blue oytster but ive refrigerated blue oytster mycelium before with no issue
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Ferather
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Re: Test Request | Caffeine LC [Re: Forrester]
#23951124 - 12/23/16 08:59 AM (7 years, 1 month ago) |
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Quote:
Forrester said: Yeah, I almost guarantee you that slime and smell was because the culture was dead and the contam's had taken over. Like you said, it was probably already contaminated to begin with, but just so you know, do some research before refrigerating your next pink oyster culture, it may not be the best idea.
I already knew that it was meant to die below 10°C, and yes it was already infected. It had stalled before the fridge. However I will let you off because no doubt you have not been following my tests and experiments.
I was testing an agar cleanup method with several dirty cultures.
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Ferather
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Re: Test Request | Caffeine LC [Re: Ferather]
#23951351 - 12/23/16 11:02 AM (7 years, 1 month ago) |
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Managed to salvage it from the outdoor bin, I added another outer bag before I brought it in. Outdoor temps also drop below 10°C, I have taken images, It may still be salvageable.
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Forrester
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Quote:
mike-ologist said: I dont know if theres a different rule for blue oytster but ive refrigerated blue oytster mycelium before with no issue
Off topic, but to clarify, yeah blue oyster is a TOTALLY different, non-tropical culture.
With blues, you can take a substrate with partially grown mushrooms, throw it outside in the winter and let the fruits freeze, bring it back inside, and they will continue to grow. Blues are the MOST cold hardy of all the oysters that I know of.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Ferather
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Re: Test Request | Caffeine LC [Re: Forrester]
#23970163 - 12/31/16 08:34 AM (7 years, 30 days ago) |
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Isn't there two types of blue? Cold blue and Warm blue.
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Forrester
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Re: Test Request | Caffeine LC [Re: Ferather]
#23976100 - 01/02/17 12:58 PM (7 years, 28 days ago) |
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Quote:
Ferather said: Isn't there two types of blue? Cold blue and Warm blue.
I guess, if someone says there is 
The strain I use fruits anywhere from just above freezing to 90F, so I guess it's kind of a cold AND warm blue. Better quality fruits below 70 though. But some of the biggest mushrooms I've gotten when I toss the buckets outside in the mid-summer heat.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Ferather
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Re: Test Request | Caffeine LC [Re: Forrester]
#23976117 - 01/02/17 01:06 PM (7 years, 28 days ago) |
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I've managed to fruit Golden oyster at 8°C, outdoors, so that sounds right to me. I have also had Summer oyster push some pins at 10°C, outdoors.
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