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OfflineAgarStudent
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Is this cobweb?
    #23915339 - 12/11/16 06:52 AM (7 years, 1 month ago)

Hi guys -

I have some concerns about contamination and do not know if this is cobweb mould, or just a variant of mycelium formation.

It is a tray of panaelous cyans.

Any feedback would be very helpful :smile:



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Offlineenlightenment
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Re: Is this cobweb? [Re: AgarStudent]
    #23915360 - 12/11/16 07:14 AM (7 years, 1 month ago)

It looks like Dactylium :frown:

Observe it but I am pretty sure it is cobweb.


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OfflineAgarStudent
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Re: Is this cobweb? [Re: enlightenment]
    #23915375 - 12/11/16 07:29 AM (7 years, 1 month ago)

Would you throw the tray away or try and treat it with hydrogen peroxide?

Cheers

Here is another tray fruiting that looks less promising?



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Why reinvent the wheel when someone here has already done the science?



Edited by AgarStudent (12/11/16 07:31 AM)


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Offlineenlightenment
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Re: Is this cobweb? [Re: AgarStudent]
    #23915384 - 12/11/16 07:33 AM (7 years, 1 month ago)

You can try to knock it back with peroxide but IME you'll loose the fight. If there would be pins already, you could try to win some time by using peroxide. But it comes back after some time.

What casing material do you use? I like to use PH adjusted casings when I grow species that take some time to fruit.


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OfflineAgarStudent
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Re: Is this cobweb? [Re: enlightenment]
    #23915407 - 12/11/16 07:49 AM (7 years, 1 month ago)

Its a peat moss, calcium carbonate and gypsum casing, as on RR's videos.  I am not sure about the pH though?

I may have got the casing a bit wet, but the best luck so far I have had with trays that were both assembled and cased at once and only fruited when the casing was colonised.

I have a fan going too, so there is plenty of FAE.

I am not sure what else could have caused it?  Casings could be kept drier?


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Offlineenlightenment
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Re: Is this cobweb? [Re: AgarStudent]
    #23915416 - 12/11/16 07:55 AM (7 years, 1 month ago)

Lack of FAE is the leading cause of cobweb. So I wonder why it grows on your casing...

Spray the cobweb with 3% peroxide in the morning and before you go to sleep. Give it a try. Maybe you have success and get some fruits. Don't mist the trays too much. Especially with Panaeolus.  Raise the FAE even more (keep an eye on your humidity).


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OfflineAgarStudent
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Re: Is this cobweb? [Re: enlightenment]
    #23915463 - 12/11/16 08:15 AM (7 years, 1 month ago)

I threw away the tray with obvious pronounced cobweb that had pins on it, but if there are others that I think are suspect, but not obvious, but have not pinned yet, then are you a bit more confident with the peroxide?

It is not very easy where to draw the line due to the difficulty of distinguishing mycelium from cobweb!

Are healthy trays at much risk in the proximity of 'suspect' trays?  Can you lose an entire crop with it easily? :confused:

Thanks for your help though.. Do appreciate it


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Edited by AgarStudent (12/11/16 08:23 AM)


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InvisibleMad Season
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Re: Is this cobweb? [Re: AgarStudent]
    #23915721 - 12/11/16 09:33 AM (7 years, 1 month ago)

Quote:

AgarStudent said:
Are healthy trays at much risk in the proximity of 'suspect' trays?  Can you lose an entire crop with it easily? :confused:

Thanks for your help though.. Do appreciate it



No they're not at risk, if they're actually healthy.. I disagree that the leading cause of cobweb is bad FAE. If you get ANY contamination, your substrate was too weak to fight off shit. This is spawn related.


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Offlineenlightenment
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Re: Is this cobweb? [Re: Mad Season]
    #23915769 - 12/11/16 09:46 AM (7 years, 1 month ago)

"The leading cause" was not the best description. That's true.
The main reason for any contam is usually bad spawn.
I meant that cobweb almost can't grow if the FAE is high (of course that does not change the fact, that the spawn might be bad).


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InvisibleMad Season
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Re: Is this cobweb? [Re: enlightenment]
    #23915782 - 12/11/16 09:48 AM (7 years, 1 month ago)

Yep it thrives in low FAE :wink: haha. It looks like op has been having trouble lately getting clean spawn. Do you have any spawn jars going right now? Show some pics?


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineAgarStudent
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Re: Is this cobweb? [Re: Mad Season]
    #23916791 - 12/11/16 04:09 PM (7 years, 1 month ago)

Thanks guys -

Here are some pictures of the spawn from agar plates.
I can see one jar has a green blob too, which is suspect, but possibly trich.
Unfortunately I just gave the other jars a good shake, so you can't see the colonisation rate,
But this contam jar is a good indicator of what the others looked like:











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InvisibleMad Season
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Re: Is this cobweb? [Re: AgarStudent]
    #23918090 - 12/11/16 10:03 PM (7 years, 1 month ago)

Your agar looks alright, but not exactly the best quality pic lol. Your spawn looks so bacterial.. Maybe try upping your PC times by half an hour. If that doesn't work, try a different grain/brand. If you have clean transfers, and youre sterile, but every jar is going bacterial, it definitely is endospores. Sometimes grains have such bad endospore populations it doesn't matter how long you pc for, you just need to find a new cleaner source


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineAgarStudent
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Re: Is this cobweb? [Re: Mad Season]
    #23918401 - 12/12/16 01:25 AM (7 years, 1 month ago)

Ah yes.  I was having some issues with a bad pressure cooker that I recently replaced.  It did waste a bit of grain!  Thanks for giving feedback though.  Does mycelium ever sweat like that or is it typically bacterial?


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Offlineenlightenment
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Re: Is this cobweb? [Re: AgarStudent]
    #23918475 - 12/12/16 02:26 AM (7 years, 1 month ago)

Mycelium produces some heat as it grows. A temperature difference leads to condensation. Often seen by sclerotia producing species. If the jar is bacterial contaminated the grains look like they are wet and do not get colonized well. If you are in doubt you can shake the jar and check how the mycelium recovers. It's always a good idea to post a picture if you are not sure about it.


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