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OfflineMatador
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Registered: 12/11/16
Posts: 1
Last seen: 7 years, 1 month
Mycelium not growing on (old)rye grain
    #23915245 - 12/11/16 04:37 AM (7 years, 1 month ago)

Hi i'm having a problem.

i made 5 rye grain jar 3 month ago.

I innoculed 1 directly(when they are cold), everything was fine the mycelium was growing. But i tried to inoculated some other jar 1 month and 1 week ago. They are not growing at all, there is no contamination, later i tried to spray water on the grain to rehydrate them, it didn't worked. Any reason why?

(I'm planning to make again some rye grain jar again and to inoculate them immediatly(when they are cold).) is that gonna fix the problem?


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InvisibleZacksJourney
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Registered: 05/02/16
Posts: 543
Re: Mycelium not growing on (old)rye grain [Re: Matador]
    #23915255 - 12/11/16 04:54 AM (7 years, 1 month ago)

Not enough details.
MS syringe? probably bunk.

And why would you want to open a jar to mist? That's wrong in so many ways.

Read as much as you can, search for teks, take notes.

Then try again.

Be sure you have a viable source.


--------------------


ALL MUSHROOMS ARE EDIBLE, some are just only edible once.

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Offlinewtfcrazymofo
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Registered: 07/26/15
Posts: 1,201
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Re: Mycelium not growing on (old)rye grain [Re: Matador]
    #23915281 - 12/11/16 05:39 AM (7 years, 1 month ago)

See if it grows on agar first.


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Invisibler.lutece
gave Columbia her wings.
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Registered: 09/06/15
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Re: Mycelium not growing on (old)rye grain [Re: Matador]
    #23915415 - 12/11/16 07:55 AM (7 years, 1 month ago)

Quote:

Matador said:
Hi i'm having a problem.

i made 5 rye grain jar 3 month ago.

I innoculed 1 directly(when they are cold), everything was fine the mycelium was growing. But i tried to inoculated some other jar 1 month and 1 week ago. They are not growing at all, there is no contamination, later i tried to spray water on the grain to rehydrate them, it didn't worked. Any reason why?

(I'm planning to make again some rye grain jar again and to inoculate them immediatly(when they are cold).) is that gonna fix the problem?




Jars should be used within two weeks after you sterilize them. After that, the chance of contamination is much higher. This is because the grains harbor bacterial endospores; when we sterilize the grain, we can't kill all of these bacteria, but we can stunt them for a while. It's important to inoculate quickly so that the mycelium can colonize the grain before the bacteria can rebound. You can't always see bacteria in jars until mycelium is trying to grow alongside it. We can see that a jar is bacterial based on how the mycelium reacts to it (cottage cheesy or lumpy look, wet looking colonized grains, very thick opaque growth patterns, amber or rust colored metabolite secretions, etc.), but when it's just uncolonized grains, it can be much harder to tell.

Also, a spray of water isn't enough to rehydrate the grains; when they are simmered, the moisture is held inside of each individual grain. When you spray the outside, it can't absorb it through the tough hull and it just evaporates.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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