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Offlinefrankwhite85
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Registered: 04/18/16
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Last seen: 4 days, 11 hours
is this practical while cloning?
    #23914928 - 12/10/16 11:28 PM (7 years, 3 months ago)

So the best course of action for getting a fruiting strain sounds like you start with ms to agar, isolate a few transfers out to ensure clean growth, let your petri's sit until a pin hopefully forms, clone that pin, isolate some good cultures a transfer or two out from said pin, then test each of those to grain.  Say that you start from ms and just keep making transfers to isolate further, once you took a transfer from that plate can you wrap it back up with parafilm to let it sit and hope for a pin? so basically as you keep isolating you let the plates your transferring from sit after the transfer in hopes a pin will form.  I figure not every plate is going to pin so by getting a ton of plates into the pipeline it could increase chances of success.  How long can an agar pin take to form once the plate is colonized?

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OfflineThe Mycologist
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Registered: 05/06/16
Posts: 3,024
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Re: is this practical while cloning? [Re: frankwhite85]
    #23914956 - 12/10/16 11:40 PM (7 years, 3 months ago)

Spores to agar, clean agar to grain, then grab a clone of the largest shroom.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

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InvisibleJosex
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Registered: 11/13/15
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Re: is this practical while cloning? [Re: frankwhite85]
    #23914958 - 12/10/16 11:41 PM (7 years, 3 months ago)

Quote:

frankwhite85 said:
How long can an agar pin take to form once the plate is colonized?




That depends, it may form or it may not. Experienced growers say that the best invitro pins are usually those that formed before the culture colonized the entire plate, because that normally means that it is a very good fruiter... So that's something you can look for if as you say you plan on keeping lots of agar cultures in hopes of cloning invitro pins.

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InvisibleIntelligentxfruit2
I'm a teapot


Registered: 12/02/16
Posts: 50
Loc: In a tree somewhere
Re: is this practical while cloning? [Re: frankwhite85]
    #23914978 - 12/10/16 11:49 PM (7 years, 3 months ago)

imo the largest shroom is not the qualifying factor for a clone. Depending on how you grow changes what you are looking for. Plus sometimes large shrooms have a horrible pin set after cloning.

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OfflineThe Mycologist
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Registered: 05/06/16
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Last seen: 18 days, 17 hours
Re: is this practical while cloning? [Re: Intelligentxfruit2]
    #23914984 - 12/10/16 11:53 PM (7 years, 3 months ago)

Nice clustering, big, sexy, you can isolate any characteristic you want.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

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Offlinefrankwhite85
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Registered: 04/18/16
Posts: 305
Last seen: 4 days, 11 hours
Re: is this practical while cloning? [Re: The Mycologist]
    #23915117 - 12/11/16 01:02 AM (7 years, 3 months ago)

So what would be better, wait for a plate to pin then start cloning or take a plate to make LI, inoculate grain, fruit then clone a pin from a cluster?

Edited by frankwhite85 (12/11/16 01:24 AM)

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OfflineCrispykoot
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Registered: 10/16/16
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Re: is this practical while cloning? [Re: frankwhite85]
    #23915457 - 12/11/16 08:13 AM (7 years, 3 months ago)

I'm trying the same right now... My thought was to clone from a mushroom that grew in the end substrate that you want to grow in first, then test it out. Seems like that would help to have something that works in your conditions. Not sure how much that matters, but it seems to make sense.I am a noob.


--------------------






:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

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Invisibler.lutece
gave Columbia her wings.
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Registered: 09/06/15
Posts: 745
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Re: is this practical while cloning? [Re: frankwhite85]
    #23915465 - 12/11/16 08:16 AM (7 years, 3 months ago)

Waiting for a plate to pin would be a more efficient use of your resources and time if you're really set on finding that perfect strain ASAP, but taking a plate to fruit and then cloning not only gives you a better look at your strain options, but you also get fruits in the meantime. :thumbup:


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.

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Invisiblefreespeech
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Registered: 12/12/08
Posts: 1,745
Loc: PNW
Re: is this practical while cloning? [Re: frankwhite85]
    #23917794 - 12/11/16 08:28 PM (7 years, 3 months ago)

Quote:

frankwhite85 said:
Say that you start from ms and just keep making transfers to isolate further, once you took a transfer from that plate can you wrap it back up with parafilm to let it sit and hope for a pin?



Yes.

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