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OfflineAK1000
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What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead * 9
    #23913095 - 12/10/16 01:15 PM (7 years, 1 month ago)

Hey everybody! So i wanted to make a post where I share all the things that have been working for me.

My first few months was full of failures and successes. I guess the failures were a good thing, because it forced me to improve my technique. It has also made me a lot more zen and patient. Now I want to share everything that has allowed me to have success thus far.

I first started getting into growing shrooms with the intention of growing "Penis Envy" cubensis mushrooms in bulk because of what a profound trip I had due to ingesting them. I figured it would be great to grow some of my own and that's how I got into this rabbit hole.



Major Mistakes

  • Well, first major mistake: I didn't order spores from a reputable vendor from shroomery. I found a sub on reddit called r/sporetraders where people sell spore syringes and stuff. I received what seemed to be a legit syringe of PE, but I never had it make mushrooms in the end, and I realized all my problems stemmed from the fact that my source for the fungi was not verified to be good from a reputable vendor.

  • Second major mistake: Using large spawn bags. I really liked the idea of colonizing rye berries and that was fine but then I started reading about spawn bags as a way to make massive amounts of spawn. What I didn't realize was that it would take forever for it to be full colonized and they get damn hot, too hot, in the summertime from their own internal heat.




  • Third major mistake: Not using agar. When I first started, I simply injected the multispore syringe into a jar of grain (rye berries).  It grew. But my jars or bags oftentimes grew other organisms, or what seemed like bacteria was infused with the fungi. (It's kind of cool though that because of  my bad experience, I can immediately tell if a bottle is contaminated within a few seconds.)

    Example of contaminated shit:

  • Fourth major mistake: Making damionteks verm/coir tek for a monotub with questionable spawn.  After I had 5 quarts of spawn, I created my first monotub using damionteks coir/verm tek, which everyone and their mother said was great and contam resistant. I got the green trich mold almost immediately. Everyone on this forum said it was due to my spawn being bad in the first place. I didn't believe them cause the spawn looked white. But oh boy, everyone was right. My spawn was not clean.

  • Fifth major mistake: Not using agar. In hindsight, this might be the most important mistake of all. Even just the thought of agar was intimidating to me. I had no idea what it was and I didn't wanna convolute my work, but IMO agar should be mandatory to learn when starting with this hobby.

  • Sixth major mistake: Constantly isolating agar sectors without knowing if it will produce fruits. When I was first getting into agar, I read stro's tutorial on isolating on agar. Well, turns out, just because you're isolating the fastest growing mycelium, isn't very useful because it doesn't mean it will produce fruits. So I ended up having clean mycelium that colonized fast but turns out it was a non-fruiting strain.
 

If I could start all over again, what advice would I give myself?




1. Get a spore syringe or spore print from a reputable/trusted vendor and DON'T get penis envy for your first grow!!!!!!! As tempting as it may sound because their potency is good, just stick to something like B+ or Golden Teachers which are very forgiving and not as finicky when it comes to pinning.

2. Get a pressure cooker.  The All American brand ones are some of the best.

3. Make a Still Air Box (SAB). This is crucial and hella cheap and cannot be skipped. From Target go get a 100qt+ clear tote and just two giant holes in it for your hands to go through. There's a good tek for making a SAB by Spitball Jedi.

  • Basic Tips for SAB SUCCESS:

  • I lay a towel soaked with soapy water for the floor and spray the inside walls of the tote with soapy water.

  • Wear a long-sleeved clean sweater with long dishwashing gloves and a simple face/air mask.

  • Work toward the back of the SAB the best you can.

  • Never let your hand hover over an open plate.


4. Start working with agar by making some agar plates (aka "pasty" plates). It's super fucking easy to make some using  Pastywhyte's easy agar tek which just uses "glad minirounds" you could get from any supermarket and agar which you could find at any asian store. This is not an optional step. If you are not convinced you need agar, you are setting yourself up for a world of hurt. Read this post by Spitball Jedi on why you should really start with agar.


  • ProTip: Make like 20-30 of these so you don't have to do the process over again and you always have some agar plates ready to go. Save the foil and papertowels as well so you could reuse them for future pasty plates.



  • ProTip #2: Use the agar recipe by Munchauzen because he gives you the measurements in grams which is super easy to measure if you have a scale and makes for a slightly less stiff agar recipe so that if you make blenderless liquid inoculant, it will break apart into more smaller pieces more easily. (More on the blenderless LI below.)


5. Put a single drop from the spore syringe on a "pasty" plate in your SAB. Do this for a few plates so you have many to choose from in case one gets contaminated for whatever reason. Also, it's hard to squeeze out only a drop sometimes, but practice makes perfect.


6. It will take at least 1 week for the spores to germinate and for the growth to be apparent when starting from a spore syringe. When you see your first signs of growth, get your scalpel and transfer a clean edge of mycelial growth onto a new plate.  Wait a few days or a week for this to grow and transfer that growth onto a new plate one more time.  It's nice to do 2-3 transfers like this to ensure that you have ONE organism growing.



7. Wait for an agar pin. Once you have an agar plate that's fully colonized, you could transfer it to some spawn, BUT...the smarter thing to do, which requires more patience, but it's really worth it, is to just leave the agar plates alone until a pin forms in the agar. (You guys gave me this advice here.) There's nothing you could do to initiate pinning on an agar plate. It just requires a ton of patience and waiting. But, when a pin forms, you know you got yourself a fruiting strain.

Here are a bunch of pins (and one giant mushroom) that have grown in a single pasty plate just sitting around:

(Note, the crazy thing about this plate is that I picked those pins in the photo and it produced 6 more pins in the meantime, and I've cloned them all.)

8. When you see the pin growing in your agar, you want to "clone" that by transferring that to a new agar/pasty plate. I used tweezers for this rather than a scalpel. 

  • ProTip: I flame the tweezers until red hot, but then before I pick the pin, I cool down the tweezers by dipping them straight into the agar (of the new plate) and then when I grab the agar pin, it won't be black and burned when I transfer it back onto that new plate.

  • When you put a pin on agar, it will revert back to the vegetative state of growth and become fuzzy with mycelium and grow out.  The cool thing is that now you have cloned something that you KNOW is going to produce mushrooms. Proof:
  • The earlier you get the pin, the more aggressively it will grow, so get a pin while it's really tiny:


9. Now that you have your agar plate that's fully colonized from a pin, you know you got yourself some badass genetics. It's now time to grow some mushrooms on a larger scale, finally! By now 8 or more weeks might have passed, depending on how long it took for the spores to germinate and how many transfers you've had to make and how long it took for an agar pin to appear.  Rather than going with growing them in a big tub, which is a valid method, I think it's better for beginners to start with the Bottle Tek. The reason for this is because each "bottle" is a single instance of the complete growing process and if one gets contaminated, it doesn't affect the other bottles. I first learned about this idea from Violets Pods Tek, (which is a great read) but the Bottle Tek seems to be the better way to go, for whatever reason. (Probably because it makes you mix coir/verm within the substrate.)

10. To make the coir-based recipe for the Bottle Tek, I got the whole oats, whole wheat and wheat bran from a local ANIMAL FEED STORE (thanks to peoples suggestion). I never been to a "feed store" in my life, but it's pretty great because I literally got 125lbs of this shit for only $46. (Fucking grass seed alone was like $10 for just a few pounds, never again will I get grass seed!) I got the verm and coco-coir and gypsum from amazon. (I also got the ziploc twist n lock bottles from amazon as well, although walmart might be the better deal.)

11. When you pressure cook the bottles, put one bottle in there that only has some water so that you could have sterilized water so that you could make blenderless liquid inoculant. This is SO FUCKING EASY, you don't need a scalpel or anything. All of you do is put the sterilized water bottle and your agar plate in the SAB and drop the puck of colonized agar in the water and shake the shit out of the bottle until the agar becomes a bunch of little pieces. If you used Munchauzens recipe for the agar, then your agar should not be too stiff and it will break ,up even easier.

  • Update: I have found MUCH, MUCH MORE CONSISTENT results making liquid inoculant (LI) using an actual blender rather than the blenderless route (shaking it up by breaking it up). I highlyyyy recommend going the blender route. Link here: https://www.shroomery.org/forums/showflat.php/Number/20429745#20429745

    Also, you don't need to modify the lid. Just use a regular mouth mason jar with an oster blender.


12. Pour a splash of this liquid inoculant into each bottle that you pressure cooked. Just a splash is enough! You will notice growth within 2-3 days with a splash.

13. Also, for the Bottle Tek, in the original write up, Mudafuka fills the bottles up to 90% capacity. But later in the thread he says how he does it only to around only 70% these days.  I can confirm that it's much better to just fill it to 70% because when you put the liquid inoculant and shake it, it will distribute all over the place rather than only being stuck at the top and it will colonize much faster that way.

Proof:

(Note how the 1 week bottle looks like it would be done in just another week.)

14. When it is colonized completely, you could put some verm on the top and open the lids and place them in a tub to act as a fruiting chamber. Choose a tub that's not too large. You want every inch of that tub to be filled with bottles so the humidity can stay high. A 60qt tub can fit 14 of those ziplock twist n lock 32oz (1 quart) bottles.

The end result will be something like this:





:sunny:

That's all I got for now. I will update this post with more knowledge as I get it, or just make another post.


Edited by AK1000 (01/24/21 11:23 AM)


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OfflineKenetic
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
    #23913106 - 12/10/16 01:19 PM (7 years, 1 month ago)

Nice


--------------------
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InvisiblemushboyMDiscord
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Kenetic]
    #23913163 - 12/10/16 01:41 PM (7 years, 1 month ago)

i like how you focused on what NOT to do. ive learned a lot by
sharing my mistakes. good write up!!

with tip number 5, 1 drop of spores.. by accident i dented a syringe needle
and it 'bottle necks' the solution. makes 1 dropping spores really really easy.
no more lookin at the cc's to see how much you are using only to apply to much
pressure and shoot 1 full cc into a jar.


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OfflineWeavieWonder
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: mushboy]
    #23913190 - 12/10/16 01:50 PM (7 years, 1 month ago)

Those bottles look great!  Definitely on my list of things to try.


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Invisibleegobrain


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: WeavieWonder]
    #23913354 - 12/10/16 02:44 PM (7 years, 1 month ago)

Awesome write up man. Definitely bookmarked. I'm about to start agar work and will be referencing this as I go. :thumbup:

5 shroomies for you :mushroom2::rockon:


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OfflinePanarchist
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: egobrain]
    #23913415 - 12/10/16 03:03 PM (7 years, 1 month ago)

great write-up. i've been meaning to do something like this as well. i think you may be violating the rules with your first point though.


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Invisibleegobrain


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Panarchist]
    #23913470 - 12/10/16 03:27 PM (7 years, 1 month ago)

Quote:

Panarchist said:
great write-up. i've been meaning to do something like this as well. i think you may be violating the rules with your first point though.




I think he's alright since he didn't actually name the vendor he ordered from.

Quote:

RogerRabbit said:
3.  Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself.  Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning.  Do not plug non sponsors.


Edited by egobrain (12/10/16 03:29 PM)


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Offlinekeifnnugs
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: egobrain]
    #23913709 - 12/10/16 04:23 PM (7 years, 1 month ago)

This is a great write up..

I make a bunch of pastys and store them as well. I also save the foil and paper towels..


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OfflineAK1000
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: keifnnugs]
    #23914275 - 12/10/16 06:59 PM (7 years, 1 month ago)

Quote:

keifnnugs said:
This is a great write up..

I make a bunch of pastys and store them as well. I also save the foil and paper towels..




Ah yes, I forgot to mention. When I run out of paper towels and the old ones are too weak to reuse, I will be using those ROUND COTTON makeup applicators instead as pastywhyte says they will last a long time!



I'm glad you guys liked the write up. I know I have more tips in my head. I'll try to share more of them.


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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OfflineJunior Fungus
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
    #23914957 - 12/10/16 11:41 PM (7 years, 1 month ago)

Great write-up! Lots of very good tricks that I've been learning the hard way like you.


--------------------
My Grow Logs:    Psychoactives:mushroom2:      Edibles & Medicinals 


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InvisibleAllDay420
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Junior Fungus]
    #23915154 - 12/11/16 01:40 AM (7 years, 1 month ago)

Great thread idea man.

It's always good to study others mistakes so you don't make the same mistakes as someone else.

Don't just learn from your mistakes, learn from others mistakes as well. :smile:


--------------------
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Invisibler.lutece
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AllDay420]
    #23915485 - 12/11/16 08:21 AM (7 years, 1 month ago)

I feel like this post should be required reading for new members. It's a perfect example of the trials a noob will go through if they don't feel like taking forum advice.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineAK1000
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: r.lutece]
    #23915681 - 12/11/16 09:22 AM (7 years, 1 month ago)

Quote:

r.lutece said:
I feel like this post should be required reading for new members. It's a perfect example of the trials a noob will go through if they don't feel like taking forum advice.




Thanks man!

Another Mistake I forgot to mention: Initially I bought a spray bottle to mix bleach and vinegar and some other nasty chemicals. DON'T DO THAT! I was using them to bleach bomb the whole room/closet and in the Still Air Box. Bleach bombing is NOT necessary and it's HORRIBLE because it's HELLA irritating to the lungs and skin and eyes!
  • If your place is hella dirty, then maybe you could get some clorox spray to wipe everything down, but it's probably not necessary to do that often.

  • And honestly, a spray bottle filled with SOAP AND WATER is enough for the SAB.  The SAB doesn't need to be STERILE. It needs to use the concept of STILL air and by spraying the inside with soapy water you effectively minimize the amount of stuff floating inside.

  • The other thing that you will use are 91% Rubbing Alcohol and papertowels, which you could get from any pharmacy, is the other product that's useful for wiping the lids before handling them and your own gloves.

  • And if you've cleaned your grow-area and it has ZERO clutter and you've wiped the floors and all the surfaces clean and you think the AIR in your room is full of spores and nasty shit, get this fogger. It will kill mold/fungi in the room. You turn it on and get out of the room until it finishes emptying all its contents and it will sterilize the air in your room.


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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OfflineAK1000
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
    #23915722 - 12/11/16 09:33 AM (7 years, 1 month ago)

The other thing I would like to add is that when you start making agar plates with the pasty-plates... one of the benefits is that it's super easy to "plop out" the entire piece of agar cause the plastic is bendable. If you want to colonize some grain, you could use a wedge of agar OR you could use the entire thing. But instead of just putting one giant piece in there, you could do what's known as the TIGER DROP 2.0:

You simply cut your agar up as if you're about to play tic tac toe, like this:



Then, when you want to inoculate some grain, you simply put the pasty plate over the jar of grain and squeeze it and it will plop out in 9 pieces with just one single motion.  Then you shake your jar and it will have 9 inoculation points.

I've stopped doing this because I'm making blenderless liquid inoculant instead, but the few times I did the tiger drop, I had extremely vigorous/fast growth and I highly recommend it.


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


Edited by AK1000 (12/11/16 09:36 AM)


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Invisibler.lutece
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
    #23915738 - 12/11/16 09:40 AM (7 years, 1 month ago)

One tip I have heard as far as using agar wedges (or the TD2.0) as inoculant is to allow it to colonize the top of the grains for a day or two and then shake; the myc leaps off a little stronger and the agar is less likely to get stuck to the sides of the jar.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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Offlinemrmazdarx9
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: r.lutece]
    #23916011 - 12/11/16 11:12 AM (7 years, 1 month ago)

Nice write up dude made alot of the same mistakes I did cool to see im not alone :highfive:


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Invisibleegobrain


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
    #23916019 - 12/11/16 11:17 AM (7 years, 1 month ago)

Quote:

AK1000 said:
Quote:

r.lutece said:
I feel like this post should be required reading for new members. It's a perfect example of the trials a noob will go through if they don't feel like taking forum advice.




Thanks man!

Another Mistake I forgot to mention: Initially I bought a spray bottle to mix bleach and vinegar and some other nasty chemicals. DON'T DO THAT! I was using them to bleach bomb the whole room/closet and in the Still Air Box. Bleach bombing is NOT necessary and it's HORRIBLE because it's HELLA irritating to the lungs and skin and eyes!
  • If your place is hella dirty, then maybe you could get some clorox spray to wipe everything down, but it's probably not necessary to do that often.

  • And honestly, a spray bottle filled with SOAP AND WATER is enough for the SAB.  The SAB doesn't need to be STERILE. It needs to use the concept of STILL air and by spraying the inside with soapy water you effectively minimize the amount of stuff floating inside.

  • The other thing that you will use are 91% Rubbing Alcohol and papertowels, which you could get from any pharmacy, is the other product that's useful for wiping the lids before handling them and your own gloves.

  • And if you've cleaned your grow-area and it has ZERO clutter and you've wiped the floors and all the surfaces clean and you think the AIR in your room is full of spores and nasty shit, get this fogger. It will kill mold/fungi in the room. You turn it on and get out of the room until it finishes emptying all its contents and it will sterilize the air in your room.





Chlorine gas..  :stirthepot:


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Offlinewtfcrazymofo
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Kenetic] * 1
    #23916730 - 12/11/16 03:47 PM (7 years, 1 month ago)

The less potent iso alcohol is better because it does not dry out as fast.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflinecronicrFacebook
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: wtfcrazymofo]
    #23916748 - 12/11/16 03:55 PM (7 years, 1 month ago)

No it works because of the higher water content.


--------------------

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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: wtfcrazymofo]
    #23916754 - 12/11/16 03:57 PM (7 years, 1 month ago)

I"m nowhere near an experienced cultivator, but I've got a good system that works consistently.  Most important things I learned by far is:

1. You gotta find the right strain/culture for your growing method.  Once you do, that's  half the battle.  I grow mostly invitro and it doesn't work well with all strains ... but it does work well with some of them.
2. RGS is, for me, the best way to grow, but I'm not cranking out pounds and pounds and have no desire for bigger grows.  $1 worth of grain grows a lot of fruits without needing any other ingredients.
3. Muda's and Violet's bottle tek is incredibly easy and efficient ... With bottles and a hood, don't even need separate fruiting chambers.  Great way to grow stealthy with extremely low contam rates.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

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OfflineHuskies
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: KauaiOrca] * 1
    #23917011 - 12/11/16 05:01 PM (7 years, 1 month ago)

I'm just piping in, I put off learning agar for the longest time. Now that I have I much clean spawn with no metabolities and my flushes have increased. Pastyplates are so easy to make, it is so worth it.


--------------------
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OfflineKenetic
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Huskies]
    #23917169 - 12/11/16 05:47 PM (7 years, 1 month ago)

Agar is awesome


--------------------
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Offlinespore-ty
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Kenetic]
    #23918728 - 12/12/16 07:00 AM (7 years, 1 month ago)

Quote:

cronicr said:
No it works because of the higher water content.



:whathesaid:


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
    #23919192 - 12/12/16 11:06 AM (7 years, 1 month ago)

Quote:

AK1000 said:
Sixth major mistake: Constantly isolating agar sectors without knowing if it will produce fruits. When I was first getting into agar, I read stro's tutorial on isolating on agar. Well, turns out, just because you're isolating the fastest growing mycelium, isn't very useful because it doesn't mean it will produce fruits. So I ended up having clean mycelium that colonized fast but turns out it was a non-fruiting strain.






Can this really be a problem? If I made agar plates out of a self-made spore print and made a lot of transfers until clean myc, is it probable that I could have isolated a "No-Fruit"-Strain?


Regards


--------------------
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OfflineHuskies
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AlCapone2k]
    #23919378 - 12/12/16 12:04 PM (7 years, 1 month ago)

Not probable but possible


--------------------
I call them Huskies cause you tell them to go "Mush! Mush""


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Huskies]
    #23919382 - 12/12/16 12:06 PM (7 years, 1 month ago)

thats why most people recommend wait to see a pin form on agar or do
a shoebox grow to test the culture.

i did 6 monos with a garbage culture myself. :doh: never again.


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: mushboy]
    #23919435 - 12/12/16 12:29 PM (7 years, 1 month ago)

But I have heared that not all agar plates will be able to produce pins, eventhough the culture could under bulk conditions.


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AlCapone2k]
    #23919447 - 12/12/16 12:32 PM (7 years, 1 month ago)

true.

i guess my though process is if it pins on agar it should fruit great.

or my thinking is off and im completely wrong. both are possible:cheers:


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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: mushboy]
    #23919453 - 12/12/16 12:34 PM (7 years, 1 month ago)

Since I've never had any pinning on my agar plates (but I throw them away after some while anyway) I cross my fingers now that I get fruits :laugh:


Regards


--------------------
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Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
    #23922005 - 12/13/16 09:15 AM (7 years, 1 month ago)

Quote:

AK1000 said:
For the Bottle Tek, in the original write up, Mudafuka fills the bottles up to 90% capacity. But later in the thread he says how he does it only to around only 70% these days.  I can confirm that it's much better to just fill it to 70% because when you put the liquid inoculant and shake it, it will distribute all over the place rather than only being stuck at the top and it will colonize much faster that way.





Proof:

Note how the 1 week bottle looks like it would be done in just another week.

Quote:

AK1000 said:
  • When you put a pin on agar, it will revert back to the vegetative state of growth and become fuzzy with mycelium and grow out.  The cool thing is that now you have cloned something that you KNOW is going to produce mushrooms.




  • Proof:

    Edit: Added these to the original post.


    Edited by AK1000 (12/13/16 09:29 AM)


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    Offlinetopsykretts
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23923842 - 12/13/16 06:59 PM (7 years, 1 month ago)

    Just because a vendor is sponsored by/sponsors shroomery doesn't mean anything.  I've gotten contaminated syringes from one vendor (free spores).


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: topsykretts]
        #23924778 - 12/14/16 12:50 AM (7 years, 1 month ago)

    Quote:

    topsykretts said:
    doesn't mean anything




    Fuck that man shroomery sponsors are top notch. The shroomery doesn't deal with unprofessional vendors. You got bad luck once and that's all there is to it. Support the vendors man, try sporeworks or the spore depot. Love em.


    --------------------
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    Offlinepacmanbreed
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23925533 - 12/14/16 10:50 AM (7 years, 1 month ago)

    Quote:

    AK1000 said:
    7. Wait for an agar pin. Once you have an agar plate that's fully colonized, you could transfer it to some spawn, BUT...the smarter thing to do, which requires more patience, but it's really worth it, is to just leave the agar plates alone until a pin forms in the agar. (You guys gave me this advice here.) There's nothing you could do to initiate pinning on an agar plate. It just requires a ton of patience and waiting. But, when a pin forms, you know you got yourself a fruiting strain.



    been lurking around,its just like RR cloned pins from agar i red a while ago..
    ill defenitely wait for pins on my agar.

    i wish i saw this earlier and gone for a shoormery spornsor instead.
    Too bad ive order 15 prints from sporetraders too :frown:

    thank you Brother for such a great right up. nice compiled threads like this are worth (book marking) for a begginers like me.


    Edited by pacmanbreed (12/14/16 10:57 AM)


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: pacmanbreed]
        #23925546 - 12/14/16 10:55 AM (7 years, 1 month ago)

    15 prints? not to put you on blast but that should be in OPs post.
    ordering too many spores.

    15?? why?? just curious. are they edibles?


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: mushboy]
        #23925613 - 12/14/16 11:12 AM (7 years, 1 month ago)

    yes 6 edibles, was planning to add to my old parent's alternative diet cause they loved the oyster i was growing..

    the rest are actives.
    they sell it in auction so 4-5 prints min order.

    too bad. if i saw this thread earlier i would have gone for the hawkseye for few quality prints.


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: pacmanbreed]
        #23925626 - 12/14/16 11:15 AM (7 years, 1 month ago)

    well with 15 prints you can make like 150-200 syringes. have fun.


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: pacmanbreed]
        #23925713 - 12/14/16 11:48 AM (7 years, 1 month ago)

    Quote:

    pacmanbreed said:
    ill defenitely wait for pins on my agar.




    Just understand that this is not a rule that's set in stone. Not every variety will produce a pin in the agar. (But if it does, it's AWESOME!) If it doesn't, even after a long time has passed and you know the culture/plate is clean, put it on some grain in a bottle and try to fruit it there.

    Quote:

    thank you Brother for such a great right up. nice compiled threads like this are worth (book marking) for a begginers like me.




    You're welcome! Glad you liked it.

    I went through so many more bad choices that I forgot to mention:

    • I bought so many glass mason jars that I don't use because the ziplock plastic ones are superior.

    • I made so many SHIPs (self healing injection ports) for the lids of those mason jars that have absolutely no use to me anymore.

    • I made liquid cultures from spore syringes which were guaranteed to be failures (but I didn't know that cause I was a noob)(a big no no cause multispore syringes are usually not very clean and agar is the real deal to clean up the culture.)

    • I got efficient at doing things that were ultimately failures.


    If I could go back in time, months ago, I would have saved a lot more money if I followed the advice and I'd be way ahead, but all of this was necessary for me to learn and it was a test of my willpower and patience. Now, I'm hella zen about the whole process and it's a blessing to share this shit with others. It's the least I could do after all the wealth of info that's here.


    --------------------
    -AK

    I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

    Best resources: Bods Easy AF List / Ultimate Tek Compendium


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    Offlinepacmanbreed
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23925916 - 12/14/16 01:01 PM (7 years, 1 month ago)

    Quote:

    AK1000 said:Just understand that this is not a rule that's set in stone. Not every variety will produce a pin in the agar.



    this is confirmed. i have oyster pinning on grain spawn now. but not on the agar plate(not yet an isolate) with the same inoculation date.
    ill transfer this pins on agar again to practice isolation while waiting for my prints.



    Quote:

    AK1000 said:
    • I bought so many glass mason jars that I don't use because the ziplock plastic ones are superior.




    defenitely worth to add on the post brother..
    i am on this route & was collecting glass jars when i began 3 months ago.
    maybe its mostly normal for a willing excited noob to think that glass only can withstand pressure cooking. :crazy:
    ill defenitely go to pp5's from now on.. i can now see their advantage.

    • i been collecting few glass petri dishes too..

    but i think their for just aesthetic. going to pp5 cups for main work from now on.



    Quote:


    AK1000 said:
    If I could go back in time, months ago, I would have saved a lot more money if I followed the advice and I'd be way ahead,but all of this was necessary for me to learn and it was a test of my willpower and patience




    defenitely right brother. ive been reading till my eyes bleeds out for a month now :eek:
    scrolling thru the good scattered info from good fellow brothers..
    seems your success is from a humble heart despite having failures..
    still had a time to pause and look back to share important compiled notes/gained knowledge despite your blessings.. to save some willing noobs time/energy/pocket specially for the old man who loves the edibles..

    ill definitely go back to this thread someday brother to look back & hoping to contribute something just like the good fellows here. from What I've Learned My First Grow.


    Edited by pacmanbreed (12/14/16 01:21 PM)


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    OfflineAlCapone2k
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23925997 - 12/14/16 01:19 PM (7 years, 1 month ago)

    Quote:

    AK1000 said:
    [LIST]
  • I bought so many glass mason jars that I don't use because the ziplock plastic ones are superior.






  • What is the advantage of these PP5-Jars compared to the glass ones?

    I can imagine that the jar lid and the threads of the plastic ones could be damaged after a while.


    --------------------
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AlCapone2k]
        #23926040 - 12/14/16 01:28 PM (7 years, 1 month ago)

    Quote:

    AlCapone2k said:
    What is the advantage of these PP5-Jars compared to the glass ones?
    I can imagine that the jar lid and the threads of the plastic ones could be damaged after a while.




    for me, pp5 are cheaper here in our place.
    glass are heavier to work with specialy for the old man.
    they can also break on handling and pressure cooking..

    i think PP5 can serve some enough grow to compensate before disposal.
    you also can go invitro on them specially bulk bottle tek on the big containers like the one used for mayonaise. some are even stackable when not in use.
    ill go bottle tek instead of mono.


    Edited by pacmanbreed (12/14/16 01:34 PM)


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: pacmanbreed]
        #23926048 - 12/14/16 01:31 PM (7 years, 1 month ago)

    yah. the pp5 containers are nice but dont last forever. unlike glass.

    but after a serious cultivation run trying to move with 100+ glass quart jars is
    a bitch.


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    OfflineAK1000
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AlCapone2k]
        #23926155 - 12/14/16 01:55 PM (7 years, 1 month ago)

    Quote:

    AlCapone2k said:
    What is the advantage of these PP5-Jars compared to the glass ones?

    I can imagine that the jar lid and the threads of the plastic ones could be damaged after a while.




    I've PC'd the plastic ones many many times and they dont break. One of them cracked cause I squeezed it unusually hard for no reason. It's a massive difference in weight when you have a PC full of glass jars versus plastic.

    But by far, the biggest advantage is that the mouth tapers wider than the bottom so the substrate will LITERALLY just slide out when you want to harvest and you just cut them off.

    It will look like this:



    Rather than this:



    That way when you want to have a second flush, you put the substrate back in easily, and dunk it in water for a couple hours and then you get another flush.

    The other MAJOR advantage is that the plastic lids are just ONE piece. When you have a jar that's colonized and you want it to fruit, you could just unscrew the lid and it will allow gas exchange to occur.  The glass mason jars, the lids and rings are separate pieces. If you unscrew the ring, the lid stays closed over it, so that's why people modify their metal lids.

    The other benefit is that they are stackable on top of each other and slide within each other when not in use.

    Also, I hear that side-pinning is a lot less common with the plastic ones. Plus they're reasonable in price. Stuff like that.


    --------------------
    -AK

    I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

    Best resources: Bods Easy AF List / Ultimate Tek Compendium


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    OfflineAlCapone2k
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23926188 - 12/14/16 02:06 PM (7 years, 1 month ago)

    Ah ok thank you for your answer. So most of the advantages are more for jar cultivation, when spawning to mono anyway there aren't so many advantages :smile:

    And I only use one-part metal lids anyway :smile:


    Regards


    --------------------
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    OfflineTira
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23926249 - 12/14/16 02:22 PM (7 years, 1 month ago)

    Great post man, this kind of posts really contribute to this site.


    Btw 90% alcohol does not work with germs, 65-70% works better AND you'll have more of if you dilute it .)


    --------------------
    Useful Links for Beginners

    The Basics              AMU Teks

    Frank''s Teks          Agar

    Noob Forum Reccomended Teks

    Agar for guaranteed spawn,
    Proper pasteurization for guaranteed substrate.


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Tira]
        #23928073 - 12/14/16 11:45 PM (7 years, 1 month ago)

    One more tip I'd like to add is that when you Pressure Cook bottles... their lids are loose and you need to tighten them after so that they don't get contaminated.

    So what I do is when the pressure in the PC has gone down to 0psi BUT STILL REALLY HOT, I will wear gloves and immediately open the lid of the PC and twist the bottles shut. The fact that everything is still very very hot will reduce the chance of anything surviving anyway while the lids are briefly cracked like that.

    I put them immediately into my SAB.


    --------------------
    -AK

    I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

    Best resources: Bods Easy AF List / Ultimate Tek Compendium


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23928089 - 12/14/16 11:58 PM (7 years, 1 month ago)

    I've never tried these bottles. Not having to mess with pasteurization sounds nice but the amount of pressure cooking I'd have to do in place seems intense

    Going to try these sometime :takingnotes:


    Edited by Intelligentxfruit2 (12/15/16 12:06 AM)


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    OfflineAlCapone2k
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Intelligentxfruit2]
        #23928229 - 12/15/16 02:09 AM (7 years, 1 month ago)

    The risk of contamns getting in while the agar is still really hot is not that high. I always just take the bottles out of the PC with gloves and screw the lid tight.


    --------------------
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    OfflineDactylium
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AlCapone2k]
        #23928250 - 12/15/16 02:25 AM (7 years, 1 month ago)

    Yeah bacteria aren't going to defy gravity and ninja their way around the threads. Maybe if you do something like open your pressure cooker before it cools off ya might could have issue-problems. Bottles look like a lot of fun. I've got a whole bunch of them in the closet waiting for me to get some cultures going.

    Quality post my dude :thumbup:


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    Offlineenlightenment
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AlCapone2k]
        #23928260 - 12/15/16 02:30 AM (7 years, 1 month ago)

    I always tighten my agar bottles before I PC them.
    Never had a broken bottle yet. The glass of the bottles is very thick. I used the same bottles for over 2500 dishes.



    How I do agar


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: enlightenment]
        #23928271 - 12/15/16 02:37 AM (7 years, 1 month ago)

    Yeah I also have these bottles, same size too :wink: they are great


    --------------------
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    OfflineJunior Fungus
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Dactylium]
        #23928275 - 12/15/16 02:39 AM (7 years, 1 month ago)

    Quote:

    Dactylium said:
    Maybe if you do something like open your pressure cooker before it cools off ya might could have issue-problems.




    Is it a southern US thing to say might could? It's very amusing. A friend of mine says that a lot. There's something else she says too but I can't remember. .. She's from south carolina. Or is it north carolina...


    --------------------
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    OfflineDactylium
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Junior Fungus]
        #23928279 - 12/15/16 02:45 AM (7 years, 1 month ago)

    It's totally a bible-belt thing. I'm Canadian born but grew up down here so I use the phrases ironically. I'm a huge fan of "irregardless"


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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Dactylium]
        #23928285 - 12/15/16 02:47 AM (7 years, 1 month ago)

    ❤️ much love, my fellow Canadian mushroom lover


    --------------------
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Junior Fungus]
        #23928345 - 12/15/16 04:14 AM (7 years, 1 month ago)

    Some of the most important things I've learned from all of my limited efforts is:

    1) A good clean LC of a good culture is worth its weight in gold
    2) It's not easy to get a good clean LC (you gotta follow the steps the right way)
    3) Pins from clusters onto agar is the easiest path to a strong producing culture
    4) Violet's method of farming contam free pins invitro on RGS from spores is easy for beginners (anyone can do this) as you don't need dozens and dozens of agar plates to find success.


    --------------------
    "The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

    -- Ancient Taoist Master


    Edited by KauaiOrca (12/15/16 06:49 AM)


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    OfflineKenetic
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: KauaiOrca]
        #23928581 - 12/15/16 08:25 AM (7 years, 1 month ago)

    Adding could after might is redundant.  Not a southern thing.  Nam talking aboot?


    --------------------
    Todo Cambia
       

                   
                                                    :cookiemonster::elmo:



    DMT said: Everyone know's me, they just don't know it yet


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    OfflineDactylium
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: KauaiOrca]
        #23929635 - 12/15/16 02:44 PM (7 years, 1 month ago)

    Quote:

    KauaiOrca said:
    4) Violet's method of farming contam free pins invitro on RGS from spores is easy for beginners (anyone can do this) as you don't need dozens and dozens of agar plates to find success.



    >spores
    >contam free
    Pick one.

    You don't even need dozens of plates to clean up a culture and wait for it to pin off of the agar. Agar is so fucking easy, I honestly can't comprehend why someone would avoid using it.


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    OfflineKenetic
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Dactylium]
        #23929708 - 12/15/16 03:04 PM (7 years, 1 month ago)

    I use a lot of plates sometimes.  Between trying new varieties or obtaining some monocultures from ms (which I still havent done despite how "easy" it sounds) or just cloning, I easily go through 40+ plates a week.  I have put some stuff on hold though cause Im moving. 
    Sucks cause one month of nothing puts me two months behind lol.


    --------------------
    Todo Cambia
       

                   
                                                    :cookiemonster::elmo:



    DMT said: Everyone know's me, they just don't know it yet


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    InvisibleKauaiOrca
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Dactylium]
        #23929715 - 12/15/16 03:07 PM (7 years, 1 month ago)

    Quote:

    Dactylium said:
    Quote:

    KauaiOrca said:
    4) Violet's method of farming contam free pins invitro on RGS from spores is easy for beginners (anyone can do this) as you don't need dozens and dozens of agar plates to find success.



    >spores
    >contam free
    Pick one.

    You don't even need dozens of plates to clean up a culture and wait for it to pin off of the agar. Agar is so fucking easy, I honestly can't comprehend why someone would avoid using it.




    That was actually my point.  That getting cleanest possible pins, then putting on Agar is a lot easier than doing a bunch of plates to get isolates and testing them each individually.  Agar is easy ... up to a point.  I've seen some growers that have 50 plates testing at once.  That's not easy.


    --------------------
    "The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

    -- Ancient Taoist Master


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    OfflineDactylium
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: KauaiOrca]
        #23930179 - 12/15/16 05:16 PM (7 years, 1 month ago)

    Oh well yeah, if you take the pins from the RGS and clean it up on agar I don't see an issue with this method.


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    OfflinecronicrFacebook
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: Dactylium]
        #23930186 - 12/15/16 05:18 PM (7 years, 1 month ago)

    Testing fifty plates is worth it in the long run.


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    Offlinegermanauslander
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: cronicr]
        #23930306 - 12/15/16 05:52 PM (7 years, 1 month ago)

    Thanks for the write up, from someone who hasn't started yet this is a nice thread


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    InvisibleTeemo 6T3
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: AK1000]
        #23930361 - 12/15/16 06:07 PM (7 years, 1 month ago)

    Quote:

    AK1000 said:
    If I could start all over again, what advice would I give myself?



    This made me lol for real tho :lmafo:


    --------------------
    Shrooming Is Of The Essence


    Rest In Peace Dankington


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    InvisibleKauaiOrca
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: cronicr]
        #23930514 - 12/15/16 06:52 PM (7 years, 1 month ago)

    Quote:

    cronicr said:
    Testing fifty plates is worth it in the long run.




    If you're a commercial level grower doing a lot of bulk grows striving for ultra peak efficiency, I would agree with you.  To get a good grow at a hobbiest level, it isn't necessary.  My results are that 50% (at least) of healthy pins snagged from a nice cluster then cleaned on Agar will generate very respectable grows.  To go to the next level of isolates and all the plates and testing necessary is a significant step in complexity and #'s of tests needed.


    --------------------
    "The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

    -- Ancient Taoist Master


    Edited by KauaiOrca (12/15/16 07:02 PM)


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    Offlinesynthetik
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: KauaiOrca]
        #23991690 - 01/08/17 07:01 AM (7 years, 22 days ago)

    thanks for this post, seriously...


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    OfflineKnockoutartist
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    Registered: 08/09/17
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    Re: What I've Learned My First FOUR Months. Full write up of my mistakes and what to do instead [Re: synthetik]
        #24599528 - 09/02/17 11:47 PM (6 years, 4 months ago)

    Subbed for more updates.


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