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OfflineKaimana
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Registered: 09/29/16
Posts: 5
Last seen: 6 years, 9 months
Contam from vendor
    #23910747 - 12/09/16 04:19 PM (7 years, 2 months ago)

Aloha, long time lurker, one successful grow, first post. I've just started this wonderful hobby, my first syringe I inoculated 5 jars one day and 5 more couple days later saw growth, got excited but it was light tan and turned to darker as colonized then dark green. All ten were done during two different days and showed the same so sadly tossed em. Used a different syringe in another round I got beautiful white mycelium, so pretty, now that grow is likely on last flush. Couple weeks ago I used different syringe and got same contamination as first round. All syringes came from same vendor and all were different"strains". Any input on what this contam is would be great, and should I contact vendor? At first I blamed myself for the failure but with the same contam repeated on two full syringes and at every inoculation point I believe syringe was contaminated. First round was in still air box, other three were done in sealed glove box, wipe with alcohol, flame sterilize, etc... I'm overly sterile if anything at the moment.

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InvisibleMad Season
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Registered: 09/16/12
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Re: Contam from vendor [Re: Kaimana]
    #23912435 - 12/10/16 08:21 AM (7 years, 2 months ago)

Still air box is always better than a glove box. Adding gloves to the box hinders sterility. Do some searching from trusted cultivators and you'll see what I mean.

It absolutely sounds like a bad syringe. When you do contact the vendor, say something along the lines of, "I looked at the spores under the scope, and I just see a ton of aspergillus" they should technically send you replacements.


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