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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
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Agar 2nd Transfer; Wheetabix
#23908989 - 12/09/16 01:06 AM (7 years, 2 months ago) |
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As per Mycolorado suggestion I have changed my medium from CFA to a cereal based nutrient. I have used a lower concentration as per his suggestion and Wheetabix ground to a fine powder as the main nutrient.
These are pictures from that experiment and I must say I am impressed
Pictures are from 1st December
Agar transfers; 22nd November
Agar plates grew to this size in just 9days from transfer to picture time
Agar plates that were used

2nd Transfers


I did a 3rd transfer from the above plates onto new plates using the same recipe. Today I will check on them and provide pictures later on
Thank you very much Mycolorado, your suggestion worked brilliantly
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Re: Agar 2nd Transfer; Wheetabix [Re: Rosen_Rot]
#23909136 - 12/09/16 04:45 AM (7 years, 2 months ago) |
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Cool beans
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   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.โ โ Maria Sabina
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 11 months
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Re: Agar 2nd Transfer; Wheetabix [Re: Nobler Hino]
#23909330 - 12/09/16 07:25 AM (7 years, 2 months ago) |
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Nice
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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Re: Agar 2nd Transfer; Wheetabix [Re: Rosen_Rot]
#23909656 - 12/09/16 10:09 AM (7 years, 2 months ago) |
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Hey Rot,
Those plates are day and night. Keep up the good work!
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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 1 month
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Re: Agar 2nd Transfer; Wheetabix [Re: Mycolorado] 1
#23909723 - 12/09/16 10:35 AM (7 years, 2 months ago) |
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
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Re: Agar 2nd Transfer; Wheetabix [Re: Mycolorado]
#23910099 - 12/09/16 12:47 PM (7 years, 2 months ago) |
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Thanks buddy!! Your push was certainly in the right direction, if you have any interesting links for me to read up pass them my way
I will be opening a journal entry for this one. Hopefully I'll continue working on a couple of isolates, I want to have 5 isolates by the end of this experiment and then test out their grow
Currently testing a wild species of P. eryngii on this mediun too, crossing fingers for a good result
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Agar 2nd Transfer; Wheetabix [Re: Rosen_Rot]
#23910249 - 12/09/16 01:36 PM (7 years, 2 months ago) |
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Dude, keep us posted on the wild Eryngii! Can I ask about the specimen, what was it growing on and environment like?
Edited by Marty Mycfly (12/09/16 01:36 PM)
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Re: Agar 2nd Transfer; Wheetabix [Re: Mattisfat] 1
#23910401 - 12/09/16 02:20 PM (7 years, 2 months ago) |
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Quote:
Mattisfat said:

I think i just had a grand mal seizure
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   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.โ โ Maria Sabina
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
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Re: Agar 2nd Transfer; Wheetabix [Re: Marty Mycfly]
#23911863 - 12/09/16 11:58 PM (7 years, 2 months ago) |
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This species usually grows on the buried degrading stalks of Ferula communis but I have found it growing some other softwoods as well. I found it growing in numerous environments, soils ranging from strictly sandy/silt to heavily clay/loam, found it in garigue environments exposed to salinity, pine plantations...etc This specific specimen I found it growing between the cracks of tarmac on the side of a road with a rubble wall, there was a carob tree hanging above and no Ferula plant nearby
The specimen I used was infested by maggotts but I managed to take undisturbed samples deep within the basal stalk and edge of the cap using clean tissue found inside after being cleaned from maggotts, bugs...etc
Hopefully it works out and takes up to the wheetabix, IME it's mycelium is quite voracious
Here is a pic of the species I found last year, it's not the same specimen that is growing and this was growing in a heavy clay based loam soil in a pine plantation
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Agar 2nd Transfer; Wheetabix [Re: Rosen_Rot]
#23912152 - 12/10/16 04:39 AM (7 years, 2 months ago) |
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Quote:
Rosen_Rot said: This species usually grows on the buried degrading stalks of Ferula communis but I have found it growing some other softwoods as well. I found it growing in numerous environments, soils ranging from strictly sandy/silt to heavily clay/loam, found it in garigue environments exposed to salinity, pine plantations...etc This specific specimen I found it growing between the cracks of tarmac on the side of a road with a rubble wall, there was a carob tree hanging above and no Ferula plant nearby
The specimen I used was infested by maggotts but I managed to take undisturbed samples deep within the basal stalk and edge of the cap using clean tissue found inside after being cleaned from maggotts, bugs...etc
Hopefully it works out and takes up to the wheetabix, IME it's mycelium is quite voracious
Here is a pic of the species I found last year, it's not the same specimen that is growing and this was growing in a heavy clay based loam soil in a pine plantation

 
boletus of steppes!!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Agar 2nd Transfer; Wheetabix [Re: blindingleaf]
#23912965 - 12/10/16 12:19 PM (7 years, 2 months ago) |
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Wow dude, gorgeous. I'm super jelly No, but really Eryngii is my favorite mushroom, it's really cool to see it in it's natural environment. If you run into them again I would love to see more pictures of them growing wild
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