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Offlinetankeras
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Stalling colonization of PF Tek jars - help me identify the reason
    #23909818 - 12/09/16 11:11 AM (7 years, 2 months ago)

My first 2 go's at PF Tek didnt work out all too great. All 12 of my cakes seemed to start off colonization really well, until maybe 3-5 days in at which point they just stopped. Out of the 12 I only managed to birth 3 disfigured excuses of cakes, which provided me with some harvest, but obviously if I achieved full colonization the yield would be much higher. I'm going to describe my method so you can hopefully point out some possible tweaks that I can do. I used normal non-organic brown rice, which the lady at the store milled for me. I used water from the tap. My vermiculite is this coarse. The recipe I used is:

1st batch:
140 ml vermiculite
40 ml BRF
water until full saturation is achieved

2nd batch:
2:1:1 - vermiculite : BRF : water

(opinions on which is superior?)

I use glasses instead of jars (cuz Europe) The first time I put 2 layers of foil on top, and the second - only one layer (so the inoculation holes were not covered). I did that as I suspected restricted airflow might have been the culprit. Do you think I should use 1 or 2 layers for the next try?

Sterilization was achieved by steaming the jars in a pot for 90 minutes. This is one of the possible reasons for the failure in my opinion. As I was using foil to cap the glasses, I'm guessing that water vapor from the pot could've gotten into the glasses and ruined my water concentration. Do you feel that's possible and what could I do to prevent it?

I inoculated with 1 ml of spore solution in 4 places. All of the jars germinated so I guess the spore syringe's name is cleared. I built a incubator which kept the air around them at 25-26 degrees. I checked on them every day, fanning the air and checking if the temp was right.

So after describing my method what pointers can you give me for my next grow? I know it's a long read, but I would be very grateful if someone more knowledgeable could lend me a hand. Thanks!

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Offlineenlightenment
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909838 - 12/09/16 11:17 AM (7 years, 2 months ago)

Quote:

I use glasses instead of jars (cuz Europe)



???

Quote:

I built a incubator which kept the air around them at 25-26 degrees]I built a incubator which kept the air around them at 25-26 degrees



unnecessary if the temperature in your room is 17°C+.

Read this thread.

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Invisibleamidogen
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Registered: 05/07/16
Posts: 1,782
Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909846 - 12/09/16 11:20 AM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:56 PM)

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Offlinetankeras
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: enlightenment]
    #23909850 - 12/09/16 11:21 AM (7 years, 2 months ago)

Generally you cant find leveled jars in Europe, they all have a narrower opening. Also, I already built a incubator, so I might as well use it to speed up the process, right?

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Invisibleamidogen
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Registered: 05/07/16
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909863 - 12/09/16 11:25 AM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:56 PM)

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Offlineenlightenment
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909866 - 12/09/16 11:25 AM (7 years, 2 months ago)

Don't use the incubator. It is not neccessary, dries out your substrate and adds a higher contamination risk.

In which country do you live? It is easy to find jars like these in Europe:

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Offlinetankeras
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: amidogen]
    #23909869 - 12/09/16 11:26 AM (7 years, 2 months ago)

In my 22 years of living here I have never seen a leveled jar here. Trust me, I've looked. I've seen other posts about it as well. My glasses are 250 ml in volume.

I used 1 ml of solution per jar - 0.25 per hole. You're saying thats outdated? Hm, there's one thing I will be changing for the next grow, thanks!

What about the steaming part - do you think I need to be worried about that screwing up my water ratio?

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Offlinetankeras
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909879 - 12/09/16 11:30 AM (7 years, 2 months ago)

Ok, based on all of your responses, I won't be using the incubator anymore- my room temperature is 20 degrees celsius (68 Farrenheit) so it should be sufficient. I live in Bulgaria, but I would be grateful if you could provide me with a link to a online store in the UK or germany which sells those jars.

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Invisibleamidogen
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Registered: 05/07/16
Posts: 1,782
Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909891 - 12/09/16 11:34 AM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:56 PM)

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Offlineenlightenment
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909894 - 12/09/16 11:35 AM (7 years, 2 months ago)

Google for "Sturzgläser" / look at ebay and try to find a shop that ships to Bulgaria.

They are cheap. You can get 100 jars for 45 EUR + shipping.

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Offlinetankeras
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: enlightenment]
    #23909912 - 12/09/16 11:43 AM (7 years, 2 months ago)

I guess I didn't look hard enough after all :laugh: Thanks - those look great and I'm positive I can ship them here.

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Offlinetankeras
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909916 - 12/09/16 11:46 AM (7 years, 2 months ago)

One more thing- what do you think about my vermiculite- is it too coarse and should I adjust the water ratio somehow or is it fine as it is?

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Offlineenlightenment
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Re: Stalling colonization of PF Tek jars - help me identify the reason [Re: tankeras]
    #23909939 - 12/09/16 11:51 AM (7 years, 2 months ago)

IMO finer vermiculite is better for cakes. I haven't made cakes for a long time... but I am sure the vermiculite you use, will work, too.

I posted a link in my first answer. Read this thread and use the BRF/verm/water ratio that is recommended there.

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