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NightPuma1
Stranger

Registered: 03/28/16
Posts: 310
Last seen: 2 years, 10 days
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First time doing agar, immediate fail...
#23909207 - 12/09/16 05:56 AM (7 years, 1 month ago) |
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So I tried to do a no-pour tek using OPB's Reusable petri dish tek
https://www.shroomery.org/forums/showflat.php/Number/21907688
which is pretty much the same as any other no-pour tek except the petri dishes are made of aluminum so they can be reused. I used the following recipe:
1/2 cup water 1g of Honey 2g of Agar 3g of Potato Flakes
Mixed all together, heated on stove until just starting to boil, poured into 10 petri dishes and let solidify into a gel at the bottom. Then I wrapped them in tin foil and threw them in my pressure cooker for 15 PSI for 35 minutes.


Result, as you can see from the pictures, is that a lot of the agar seemed to boil out of the little containers (yes they were put in upright). And indeed I see a lot of it in the bottom of the pressure cooker.
The guide just says to throw them in your pressure cooker, but there is like 2 inches of water along the bottom so are they supposed to just float in there because that's what I did. Perhaps the mixture boiled and flowed out of the pre-drilled hole because of this? If this is the case, do I need to put something that floats in there and then place the dishes on top of it so they don't boil? Or perhaps my recipe for the solution wasn't good? Thank you.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: First time doing agar, immediate fail... [Re: NightPuma1]
#23909235 - 12/09/16 06:16 AM (7 years, 1 month ago) |
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look up "pastywhyte easy agar" and load your PC like that next time.
they should not float, they need to be on something solid and the boiling water shouldn't be able to touch the lids.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 10 hours, 35 seconds
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Re: First time doing agar, immediate fail... [Re: NightPuma1]
#23909431 - 12/09/16 08:11 AM (7 years, 1 month ago) |
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The only way I had any success with that was to individually seal my petris in poly tubing...ended up being a pain in the ass...I pour petris now much easier....I wrap petris(stacks of 4) in aluminum foil and oven sterilize @425°F for 90 minutes let cool and pour agar in SAB...
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: First time doing agar, immediate fail... [Re: Leftfield420]
#23909454 - 12/09/16 08:25 AM (7 years, 1 month ago) |
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I had pretty good success with agar Id recommend get a pyrex media bottle to PC your agar in then pour in sab or in front of hood ive done all my agar in a sab and so do alot of people on here but pour from the bottom up and I stack mine in stacks of 3 just easier for me to grab all of them and pour bottom up rather quickly and smoothly
Also do the motion without the actual stuff in it to get a feel and are these glass or plastic petri?
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 10 hours, 35 seconds
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Re: First time doing agar, immediate fail... [Re: spore-ty]
#23909478 - 12/09/16 08:36 AM (7 years, 1 month ago) |
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My petris are pyrex....I need to get pyrex media bottle it would help with accidental spillage....splilling agar in a SAB is an upset...kinda like spilling the bong or some shit
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First time doing agar, immediate fail... [Re: Leftfield420]
#23909487 - 12/09/16 08:42 AM (7 years, 1 month ago) |
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I hate the pyrex dishes. Ive got three dozen of them i never use

I've also been using a 66quart sab for three years finally made a 110 sized one
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