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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: The exotic species thread [Re: Th3Issu3]
#23942563 - 12/20/16 07:31 AM (7 years, 1 month ago) |
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I often noticed that the center does not produce sclerotia. I am working on an isolate (Psi. galindoi) right now to test if the production of sclerotia can be stimulated by adding marbles to the substrate to increase the surface area. I want to do this with an isolate to get legit test results.
An interesting post of a pretty unknown user:
Quote:
XDX said: ... There is PLENTY of undigested grain in the middle of the jar (colonized, but still visible grain with visible starch etc), so I don't Beleive any low yield has much to do with lack of nutes... It's strange too because the way I understand sclerotia and their biological function is that they form in grasslands a the dry season approaches, to protect against drough. So sclerotia should form under drier conditions, yet here they are forming in the highest humidity against te glass with condensation droplets around and such, yet the middle of the jar with undigested grain is actually pretty dry... Why? What's happening here? Here's my thoughts: sclerotia Indeed form in response to drought conditions, but rather than waste energy producing modifications everywhere within their mycellial network, they focus this energy in area where they meet physical constraints/resistance, something hard that it can't easily digest.. In nature this would be a fat tree root or rock. Now, when the rain finally comes again, if you clumped up your sclerotia bodies around large hard surfaces, I would think that the rainwater would collect around and follow that hard surface according to gravity. Kinda like rain collecting and streaming off the sides of your moving car, even tho the rain isn't coming down hard at all, maybe just a drizzle. Evolved habits to help guarantee rehydration during wet season... Just my thoughts, maye I'm way off...
I shortened the quote a bit. source
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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I have a bunch of them, but they are the same height as the quart jars just thinner
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Re: The exotic species thread [Re: Peteyboy]
#23942581 - 12/20/16 07:38 AM (7 years, 1 month ago) |
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maybe instead of some marbles, you can put a glass test tube in the middle of one of those jars ?
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Another great idea, Th3Issu3. I open a new thread when I completed the agar work.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The exotic species thread [Re: Th3Issu3]
#23942606 - 12/20/16 07:50 AM (7 years, 1 month ago) |
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In that post a shot glass was a suggestion. Good ideas
Enlightenment
Have you tried this on an ms? Or have you been working out a proven isolate ? It almost seems as if they form from empty spaces between grains next to the glass. Maybe larger grains could be used to achieve the larger spacing (more stones).
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Quote:
enlightenment said: Another great idea, Th3Issu3. I open a new thread when I completed the agar work.
I'll be sure to follow man!
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Quote:
enlightenment said: I often noticed that the center does not produce sclerotia. I am working on an isolate (Psi. galindoi) right now to test if the production of sclerotia can be stimulated by adding marbles to the substrate to increase the surface area. I want to do this with an isolate to get legit test results.
An interesting post of a pretty unknown user:
Quote:
XDX said: ... There is PLENTY of undigested grain in the middle of the jar (colonized, but still visible grain with visible starch etc), so I don't Beleive any low yield has much to do with lack of nutes... It's strange too because the way I understand sclerotia and their biological function is that they form in grasslands a the dry season approaches, to protect against drough. So sclerotia should form under drier conditions, yet here they are forming in the highest humidity against te glass with condensation droplets around and such, yet the middle of the jar with undigested grain is actually pretty dry... Why? What's happening here? Here's my thoughts: sclerotia Indeed form in response to drought conditions, but rather than waste energy producing modifications everywhere within their mycellial network, they focus this energy in area where they meet physical constraints/resistance, something hard that it can't easily digest.. In nature this would be a fat tree root or rock. Now, when the rain finally comes again, if you clumped up your sclerotia bodies around large hard surfaces, I would think that the rainwater would collect around and follow that hard surface according to gravity. Kinda like rain collecting and streaming off the sides of your moving car, even tho the rain isn't coming down hard at all, maybe just a drizzle. Evolved habits to help guarantee rehydration during wet season... Just my thoughts, maye I'm way off...
I shortened the quote a bit. source
So true, great ideas guys, holy shit this gave me a wild idea of custom making a slim glass/plastic container (Like a window but with a 3 or 5 cm wide space in the middle).
And just throwing in colonised grains inside, and make a sort of trap door at the bottom which can slide off which you can just drop all the grains when you want to harvest.
Hang it like a picture on the wall, its literally a growing and live art show
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Re: The exotic species thread [Re: Teemo 6T3]
#23943178 - 12/20/16 11:46 AM (7 years, 1 month ago) |
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That would be siiiick!
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 12 hours, 11 minutes
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Re: The exotic species thread [Re: Th3Issu3]
#23943968 - 12/20/16 04:38 PM (7 years, 1 month ago) |
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Stonesun let his bags colonize not full and flat so there was more bag surface area per grain for the stones to grow.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Quote:
wtfcrazymofo said: Stonesun let his bags colonize not full and flat so there was more bag surface area per grain for the stones to grow.
GTK! Makes sense as the stones would be just sub-surface as opposed to 6 inches down....I would imagine.
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Mush 4 Brains
about tree fiddy


Registered: 12/19/07
Posts: 8,298
Loc: Tacos
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Quote:
Mush 4 Brains said:
  Got a few plates of pan cyans on mea and 4 quart jars of wbs noc'd up with a little bit of agar if that qualifies for this thread. The pan myc is moving slow on agar. Very sluggish compared to cubes. I had a hunch that it would begin to take off once it hit grain.
So since i was beginning to lose patience i took a few of my plates with the most growth (only had myc growth about the size of a quarter) and noc'd up 4 jars. Ideally i would have waited til i had more substantial myc growth before transferring to grain.
The jars are less than 48hours since inoculation and already im seeing more growth than i would have on agar in that amount of time.
Also i used a print on agar to start with like i typically do with cubes. Im beginning to think that making a spore syringe first to noc up the agar instead, would have been more advantageous. Giving the spores a chance to re-hydrate and what not before going to agar. Maybe would've aided in germination which seemed to take forever. What do y'all think about that subject?
I find i almost never make spore syringes anymore or lc for that matter. Havent made any lc in ages
So my hunch that the pan cyan myce would take off once it hit grain was on point. Something about the MEA, the pans apparently didn't care for it. Really sluggish growth.
A quick search and you'll find numerous posts with people using malt extract for their pans. It does indeed work but im thinking there's something more preferable to use.
Note the growth on each of these side by side pics.
 
The pic on the left is from 11 days and the pic on the right is the dish today. As you can see it's only doubled in size in that time. In that amount of time it would've grown all the way to the end of the dish and began crawling up the sides looking for nutrients if it was p.cubes.
Now scope the jars out. I shook em I wanna say like 5ish days ago, now they're about done. I didn't have a whole hell of a lot of pan myce on agar initially so I only ended up noc'n up 3 quart jars(which are all looking nearly exactly the same now). So about 15 days for full colonization of a quart jar using just a small piece of myce from the agar dish, not too bad.
 
Check out this nifty little alcohol torch I found.
You just give it a little squeeze and you can direct the flame. Gets a scalpel red hot quite quickly. I really like this little torch, so convenient. A lot of times I dont even have to squeeze it. I just let is sit on the table, light it up real quick with a lighter, hold the scalpel above the flame to heat it up and just blow it out when done.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Thats cool probably not super safe but nothing cool is lol
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Thekhatinthehat
Stranger
Registered: 12/21/16
Posts: 25
Last seen: 7 years, 22 days
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Re: The exotic species thread [Re: mrmazdarx9]
#23946582 - 12/21/16 04:32 PM (7 years, 1 month ago) |
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Cool
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Re: The exotic species thread [Re: mrmazdarx9]
#23946607 - 12/21/16 04:40 PM (7 years, 1 month ago) |
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Quote:
mrmazdarx9 said: Thats cool probably not super safe but nothing cool is lol
Truest thing I've heard all day!
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Mush 4 Brains
about tree fiddy


Registered: 12/19/07
Posts: 8,298
Loc: Tacos
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Re: The exotic species thread [Re: Th3Issu3]
#23947834 - 12/22/16 12:36 AM (7 years, 1 month ago) |
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Quote:
mrmazdarx9 said: Thats cool probably not super safe but nothing cool is lol
Whats not safe? I mean FIRE HOT!! FIRE BURN TARZAN! But Of course if tarzan isnt a big dummy, everything is fine...
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Yeah but tarzan loves his booze.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: The exotic species thread [Re: Th3Issu3]
#23984923 - 01/05/17 07:20 PM (7 years, 25 days ago) |
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Well well well...got myself a whack of stuntzii jars knocked up today! here's to hope folks
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TheDuder
Mushroom Hunter



Registered: 11/07/16
Posts: 2,544
Loc: PNW
Last seen: 1 year, 18 days
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Re: The exotic species thread [Re: cronicr]
#23984931 - 01/05/17 07:23 PM (7 years, 25 days ago) |
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Did the same with cyans a few days ago
--------------------
|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|
|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: The exotic species thread [Re: TheDuder]
#23984956 - 01/05/17 07:31 PM (7 years, 25 days ago) |
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me three with some pans.
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keifnnugs
Master fucker

Registered: 11/27/15
Posts: 335
Last seen: 26 days, 9 hours
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Re: The exotic species thread [Re: mushboy]
#23984959 - 01/05/17 07:33 PM (7 years, 25 days ago) |
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Me 4 with pan tamps..
How are you guys spawning them?
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