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OfflineEdmunter
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
    #23906507 - 12/08/16 11:28 AM (7 years, 2 months ago)

I think we discussed above you need to make one more transfer to clean standard media

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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #23906512 - 12/08/16 11:29 AM (7 years, 2 months ago)

How's that grain spawn test going btw?


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
    #23906525 - 12/08/16 11:32 AM (7 years, 2 months ago)

This is not my thread, but so far positive results. Katbusa is beating me to it though.


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InvisibleLeftfield420
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Re: Cleaning dirty cultures with tea agar [Re: ComebackKid]
    #23906538 - 12/08/16 11:37 AM (7 years, 2 months ago)

Good to know... The mold must have piggybacked my mycelium then? Because I transfered from the furthest point from the visible mold...

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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #23906548 - 12/08/16 11:39 AM (7 years, 2 months ago)

Im rather fond of using the property of the agar as another member stated

Wish I coukd remember the link ill dig it up..

I noticed when using old dried out (1-2month old) plates for cloning my bacterial contamination rates plummeted. I never looked back. Just keep petris in zip lock bags.

Another member started a thread on how he likes to increase the amount of agar added to the water. Which in effect makes a much drier agar surface. Same effect I was gaining just a diff way of going about it.

I haven't experimented with this type of method and cleaning up cultures but cloning it does wonders.

I like this idea tho,  maybe start your spores on regular agar then throw mycelium onto tea agar for ur antibiotic agar effect. Then back to regular agar before u go to grains

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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Raven44]
    #23906578 - 12/08/16 11:47 AM (7 years, 2 months ago)

Quote:

Leftfield420 said:
Good to know... The mold must have piggybacked my mycelium then? Because I transfered from the furthest point from the visible mold...



I have done 4 spawn samples that have no visible mold, and then mold coming from it.


Quote:

Raven44 said:
........
I like this idea tho,  maybe start your spores on regular agar then throw mycelium onto tea agar for ur antibiotic agar effect. Then back to regular agar before u go to grains



Thank you for that information, you can use this agar however you like.


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OfflinePsilosopherr
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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #24078721 - 02/09/17 11:36 PM (7 years, 21 days ago)

wondering if anybodies been continuing to test this idea? noticed ferathers thread on the subject is no longer with us so thought I'd ask here

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Re: Cleaning dirty cultures with tea agar [Re: Psilosopherr]
    #24078933 - 02/10/17 05:21 AM (7 years, 21 days ago)

I have a jar of tea agar I've had for months with no apparent bacteria or mold in it....and I still have the tea agar petri I mouth swabbed... Little to no growth on it...definently curves the growth of bacteria.. If mold spores are introduced they will grow on tea agar though

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Re: Cleaning dirty cultures with tea agar [Re: Leftfield420]
    #24420644 - 06/20/17 01:58 PM (6 years, 8 months ago)

this is really cool mycolorado. are you still using the tea agar? i think im going to try this recipe out. also, the color really pops the mycelia. bookmarked and im adding this to my reference list.


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InvisibleMycolorado
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive]
    #24420731 - 06/20/17 02:32 PM (6 years, 8 months ago)

Hey eat!  I haven't used it since my initial dirty cultures from syringe.  Both my Malabar and APE were REALLY dirty.  I think it would be a great tool for cleaning up tissue cultures from wild specimen as well and will be giving it a try this summer with some wild oyster clones.


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OfflinePsilosopherr
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Re: Cleaning dirty cultures with tea agar [Re: Mycolorado]
    #24422111 - 06/20/17 09:15 PM (6 years, 8 months ago)

I had success cleaning a dirty culture with this method, I used green tea.

Won't need it very often in my career but I'd definitely use it again

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Re: Cleaning dirty cultures with tea agar [Re: Psilosopherr]
    #24422121 - 06/20/17 09:19 PM (6 years, 8 months ago)

Green tea worked? I might give that a try myself. Not pinning my hopes or nothing but I got a culture that I may give this a go with anyway.

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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Pastywhyte] * 1
    #24423238 - 06/21/17 08:58 AM (6 years, 8 months ago)

It works in the way of alternative carbon sources (non-sugar), 100g of black tea has 3g natural sugar no starch.
As it stands without the trace carbohydrates, it's very nutritional and soluble, even cheap black tea.

The natural carbon containing acids breakdown at about ph 6.5, so pH 7-7.5 is ideal.
You can still get some bacteria but total activity should be reduced overall.

Here is some composition links (external), Tea, Tea to drink.


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] * 1
    #24461944 - 07/06/17 12:44 PM (6 years, 7 months ago)

I setup another recipe, sugar and starch free. I am using cheap black tea, about 80p for 250g.
My setup is using a cellulose bacteria (also eats starch and sugar) as the infection.

The recipe and further explanation can be found in katbusa's thread here.
As a side note cubensis will transfer to a wood toothpick peg.


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] * 1
    #24466933 - 07/08/17 06:43 AM (6 years, 7 months ago)

Here is an export of another thread I made, related to a carbon discussion.
You can use the produced enzymes to create a controlled media.


Digestive enzymes:

Amylase - starch  []  Laccase - lignin  []  Cellulase - cellulose  []  Xylanase - hemicellulose  []  Protease - proteins.

Mycelium should only cleave the bonds of carbohydrate's and hydrocarbon's, C-H bonded units.
It should not for example degrade materials of a C-C bonding, such as coal or diamonds.

However there may be specialized mycelium that may, it will be rare.

----

Carbon-Hydrogen targeting:

Amylase, Laccase, Cellulase, Xylanase


Carbon + Nitrogen targeting:

Protease

----

Example carbon materials:

Starch, Sugar, Cellulose, Hemicellulose, Lignin, Tannins, Bicarbonates.


Example nitrogen materials:

Proteins, Vitamins, Caffeine, Ureic, Ammonical.

----

Side note:

Laccase will decay many carbon materials.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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Re: Cleaning dirty cultures with tea agar [Re: Ferather]
    #24467057 - 07/08/17 08:02 AM (6 years, 7 months ago)

It would be very cool if green tea works. id love to see how a green tea plate would look.

damnit you guys are making me do work now. i have to try this out.


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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: eatyualive] * 1
    #24467077 - 07/08/17 08:14 AM (6 years, 7 months ago)

I believe katbusa tested green tea with positive results, although a small amount of malt extract was used.
He did note the same oxidization effect around the mycelium, though less coloured, more brown.

Technically speaking green tea extract should be richer, and closer to natural.
The mycelium should have no issues oxidizing materials with laccase.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] * 1
    #24467107 - 07/08/17 08:28 AM (6 years, 7 months ago)

Identification of contamination (where CaCO3 is being used) is fairly easy, bacteria: yellow, yeast: creamy.
Mycelium, this includes molds (they are a mycelium too!), look normal when growing.

   


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] * 1
    #24467111 - 07/08/17 08:31 AM (6 years, 7 months ago)

I suggest going sugar and starch free, or at least half normal ME dose.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Cleaning dirty cultures with tea agar [Re: Ferather] * 2
    #24467226 - 07/08/17 09:22 AM (6 years, 7 months ago)

I also suggest using a sterilized wood toothpick to isolate-transfer mycelium, as it helps restrict contamination.
You can see my signature (below) to see my toothpick and cellulose agar guides, as a reference.

The above culture, overpowered present contamination eventually, a sample was sent.

Here are three posts of the results of the sent sample: One, Two, Three.
Also, here is another case of a peg transfer from UK > US.

   


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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