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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23932176 - 12/16/16 11:19 AM (7 years, 1 month ago)



Ok fellas, so I think we're at full colonization at this point. Tub was started on the 8th. Mycelium is breaking through the casing layer in a few spots. This is the first monotub I ever made, and it still has tape along the bottom, because that was the conventional wisdom at the time lol. I can't see the colonization very well because of this. I could take the tape off, but this tub is set for a lower substrate depth than the rest, and I leave it on so I can quickly find this one, if I'm doing something that requires less sub.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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Offlinetump
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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23932259 - 12/16/16 11:51 AM (7 years, 1 month ago)

Pins dam you


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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: tump]
    #23932615 - 12/16/16 02:21 PM (7 years, 1 month ago)

Quote:

tump said:
Pins dam you




Lmao! That's what I have been saying every time I look at that tub, while shaking my fist angrily. Hopefully it will comply shortly!


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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Offlinekeifnnugs
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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23932721 - 12/16/16 02:57 PM (7 years, 1 month ago)

Looking forward to seeing the outcome.. I bet you would get a better yeild taking that jar and turning it into 24 jars.. The speed alone would be worth it


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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: keifnnugs]
    #23932855 - 12/16/16 04:01 PM (7 years, 1 month ago)

Quote:

keifnnugs said:
Looking forward to seeing the outcome.. I bet you would get a better yeild taking that jar and turning it into 24 jars.. The speed alone would be worth it




I just made over 90 jars with PF slurries. This was an extra :wink:

By the way, a very significant portion of them actually did colonize in 3 days. Some of them are moving along quite a bit slower though. Not entirely sure why.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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Offlinetump
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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23933747 - 12/16/16 09:24 PM (7 years, 1 month ago)

In big bacths the myc isnt divide evenly. So splashes you pour have less active cell left or more dead cell for contams to feed on. Anything takes longer then 6 day with slurry is contamed to hell. About 20% fail rate. Whats yours been so far?


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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: tump]
    #23933944 - 12/16/16 11:16 PM (7 years, 1 month ago)

Quote:

tump said:
In big bacths the myc isnt divide evenly. So splashes you pour have less active cell left or more dead cell for contams to feed on. Anything takes longer then 6 day with slurry is contamed to hell. About 20% fail rate. Whats yours been so far?




Honestly, I have had 100% success so far. I've been doing it in front of a flowhood though, so I'm sure that helps! This last batch I made with slurry, I used two cakes that had a bunch of pins on them, so I'm sure that sped things up a bit.

In fact, one of the pf cakes I made in a glad round, the pins ended up pushing the lid up, so I'm sure contaminant spores could have gotten in there. They almost certainly did. The thing is, I knew the cake colonized clean, and given the colonization speed of slurry in jars (~3 days), there really wasn't enough time for mold to grow from spores to contaminate my spawn.

I can almost guarantee the spawn will be clean.

Honestly, I'm going to put a theory of mine to the test. I believe that I could make a fresh batch of jars, and inoculate them with slurry in open air, in my kitchen, and have a ~0% contamination rate. The colonization speed is so fast, I think it could work quite well. Think about the implication. You would have no need for a flowhood, or a glovebox, beyond making a clean PF cake.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23934139 - 12/17/16 12:39 AM (7 years, 1 month ago)

We will see. pictures


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OfflineChips
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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23934180 - 12/17/16 12:53 AM (7 years, 1 month ago)

Quote:

Bigbadwooof said:
Honestly, I'm going to put a theory of mine to the test. I believe that I could make a fresh batch of jars, and inoculate them with slurry in open air, in my kitchen, and have a ~0% contamination rate. The colonization speed is so fast, I think it could work quite well. Think about the implication. You would have no need for a flowhood, or a glovebox, beyond making a clean PF cake.




interesting idea, would love to see a thread about this


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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: Chips]
    #23934306 - 12/17/16 02:14 AM (7 years, 1 month ago)

Quote:

Chips said:
Quote:

Bigbadwooof said:
Honestly, I'm going to put a theory of mine to the test. I believe that I could make a fresh batch of jars, and inoculate them with slurry in open air, in my kitchen, and have a ~0% contamination rate. The colonization speed is so fast, I think it could work quite well. Think about the implication. You would have no need for a flowhood, or a glovebox, beyond making a clean PF cake.




interesting idea, would love to see a thread about this




Well, I will make one! :smile:

We can all see it in action. I am making 3 tubs tonight, so that will free up 15 jars, and I already have 5 empty ones. Either tonight or tomorrow, I will probably whip up some oats. Then I have to PC the jars. I could possibly give it a shot by tomorrow night.

I have one glad-round sized BRF cake. I could do it right in my kitchen, with a magic bullet, instead of the oster, if we're throwing sterile tech out the window. My only concern is any living mold mycelium getting in there. If I give my kitchen a light misting with rubbing alcohol, and take some measures to reduce airflow, I don't think this will be a problem.

Maybe I'll just do 5 jars for proof of concept first.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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InvisibleComebackKid
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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23934324 - 12/17/16 02:31 AM (7 years, 1 month ago)

Quote:

Bigbadwooof said:
Quote:

Chips said:
Quote:

Bigbadwooof said:
Honestly, I'm going to put a theory of mine to the test. I believe that I could make a fresh batch of jars, and inoculate them with slurry in open air, in my kitchen, and have a ~0% contamination rate. The colonization speed is so fast, I think it could work quite well. Think about the implication. You would have no need for a flowhood, or a glovebox, beyond making a clean PF cake.




interesting idea, would love to see a thread about this




Well, I will make one! :smile:

We can all see it in action. I am making 3 tubs tonight, so that will free up 15 jars, and I already have 5 empty ones. Either tonight or tomorrow, I will probably whip up some oats. Then I have to PC the jars. I could possibly give it a shot by tomorrow night.

I have one glad-round sized BRF cake. I could do it right in my kitchen, with a magic bullet, instead of the oster, if we're throwing sterile tech out the window. My only concern is any living mold mycelium getting in there. If I give my kitchen a light misting with rubbing alcohol, and take some measures to reduce airflow, I don't think this will be a problem.

Maybe I'll just do 5 jars for proof of concept first.




:rockon:
Start a thread
If you fail you fail
Good bad or ugly
We need some failed experiments imo
Not saying I don't have faith in this one though!
(Also if you're willing to sacrifice spawn you shold try the "anti bacterial" tea grains for good measure


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Offlinetump
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Re: Eats 1 Cake Slurry Spawn [Re: ComebackKid]
    #23934466 - 12/17/16 04:14 AM (7 years, 1 month ago)

Hard to tell my tea agar plates died. But yes experiment
Thats how the magic of slurry was discovered


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OfflineSloppyJoseph
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Re: Eats 1 Cake Slurry Spawn [Re: tump]
    #23934801 - 12/17/16 08:47 AM (7 years, 1 month ago)

I need to make a slurry kit. Been wanting one for a long time but been lazy cause blenderless works pretty great also. Double the time but the ease of use is what I like.

But I'm in here to see this pinset. Essentially half a cake to half a brick of coir is like, way less than  even reishi level spawn ratios. Im with Bod on his guess


--------------------
AMU Q&A


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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: SloppyJoseph]
    #23935192 - 12/17/16 11:51 AM (7 years, 1 month ago)

Quote:

SloppyJoseph said:
I need to make a slurry kit. Been wanting one for a long time but been lazy cause blenderless works pretty great also. Double the time but the ease of use is what I like.

But I'm in here to see this pinset. Essentially half a cake to half a brick of coir is like, way less than  even reishi level spawn ratios. Im with Bod on his guess




Oh, we shall see. It's definitely been slow going, I will say that. All the other tubs I spawned around that time are fruiting, as of today, and some have already flushed lol.

I'm still holdin out! The thing is, 3/4 of the AA+ cakes I made from that agar plate went visibly moldy. This tub hasn't molded out yet. It really is a long shot lmao!

I have to say blenderized slurry works amazingly well.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23958767 - 12/26/16 04:54 PM (7 years, 1 month ago)

:waits: Still waiting. No pins :frown:

No mold either, by the way. Even if this proves successful, I don't intend to do it again in the future.

By the way, I've decided against the slurry to grain in open air. One of the batches I made with a glad round that had the lid pushed open by pins went to mold town. I still think it might be possible, but the success rate would probably be abysmal.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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Re: Eats 1 Cake Slurry Spawn [Re: Bigbadwooof]
    #23958783 - 12/26/16 05:03 PM (7 years, 1 month ago)

:manofapproval:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblebodhisattaMDiscordReddit
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Re: Eats 1 Cake Slurry Spawn [Re: cronicr]
    #23958876 - 12/26/16 05:38 PM (7 years, 1 month ago)

Well guess it's time you make another pan mono for us to drool over


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Offlinetump
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Re: Eats 1 Cake Slurry Spawn [Re: bodhisatta]
    #23961785 - 12/28/16 01:59 AM (7 years, 1 month ago)

Slurry work great im telling you. Just not open air. Trich alway pops up on a few jarz even in a still air box. Surpised it hasnt pinned ir contam to hell


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OfflineAcquario
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Re: Eats 1 Cake Slurry Spawn [Re: tump]
    #23962404 - 12/28/16 10:24 AM (7 years, 1 month ago)

Pics? :hi5:


--------------------


Amateur Mycologists United [AMU Q&A Thread] - Ask any question here!


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OfflineBigbadwooof
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Re: Eats 1 Cake Slurry Spawn [Re: Acquario]
    #23963137 - 12/28/16 04:01 PM (7 years, 1 month ago)

Tbh, it doesn't look much different than the pic at the top of the page, but I think I may be seeing knots.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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