|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
no0bsawse
Yearnin' for Learnin'


Registered: 08/26/16
Posts: 104
Last seen: 5 years, 1 month
|
Can I really use one syringe to innoc 30+ jars?
#23905616 - 12/08/16 12:33 AM (7 years, 1 month ago) |
|
|
Hello, I have a spore syringe with half a print inside of it, and is just loaded with spores. Instead of splitting it up into many syringes, could I just squirt literally one drop per innoc hole in my BRF jars and have everything be all good?
-------------------- LF: Gourmet Strains
|
r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: no0bsawse]
#23905625 - 12/08/16 12:39 AM (7 years, 1 month ago) |
|
|
The problem with that is that you then have a single inoculation point, which will take much longer to colonize the jar. Also, I seem to remember reading that the fluid from the syringe is beneficial to the germination process, and too little fluid will stunt germination. (I can't verify that, however.)
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: r.lutece]
#23905629 - 12/08/16 12:41 AM (7 years, 1 month ago) |
|
|
Never tried (or read much into) PF tek myself but learn agar and one drop from that syringe could start one plate that could lead to a near endless amount of innoculant.
--------------------
Highwalker
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
|
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
klhouse



Registered: 12/12/15
Posts: 671
Loc: SE Virginia
Last seen: 4 years, 1 month
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: van hatton]
#23905640 - 12/08/16 12:59 AM (7 years, 1 month ago) |
|
|
All I know is, I have done jars for years with no problems. Then I thought---There are millions of spores in this syringe, I'll put the tiniest dab in each jar... I put in the smallest injection in each jar that I could and NONE of them did anything. I do think you need a certain amount.
-------------------- Shroomery mycologist definitely know their shit. Knowledge talks. Wisdom listens.
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: klhouse]
#23905722 - 12/08/16 02:59 AM (7 years, 1 month ago) |
|
|
In theory for cakes its great. Ive divided syringes into 50. Syringes with similar growth rates. with Spores less is more. But you need to still have the water to spread the growth down the cake.
|
wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 28 minutes
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: no0bsawse]
#23905760 - 12/08/16 03:44 AM (7 years, 1 month ago) |
|
|
You can make one drop spread out to as many jars as you can handle with some good old agar work. You might want to throw the pf tek out the window though.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
|
spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: wtfcrazymofo]
#23905803 - 12/08/16 04:51 AM (7 years, 1 month ago) |
|
|
Quote:
wtfcrazymofo said: You can make one drop spread out to as many jars as you can handle with some good old agar work. You might want to throw the pf tek out the window though.
Yup literally ONE or two single droplets on to each agar plate and you'll be able to do infinite jars and you'll know of the mycelium is actually clean.
|
filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: spore-ty]
#23905935 - 12/08/16 07:11 AM (7 years, 1 month ago) |
|
|
I use .5-1cc/ml of spore solution per quart, otherwise ime colonization can take far longer (I've tried the single drop)
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
|
Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: filthyknees]
#23906015 - 12/08/16 08:02 AM (7 years, 1 month ago) |
|
|
Why don't you put a small drop on a agar plate, then turn the plate into more plates and use them to do 30 jars.
Than you'll Use your needle to do 30000000 jars that way.
|
no0bsawse
Yearnin' for Learnin'

Registered: 08/26/16
Posts: 104
Last seen: 5 years, 1 month
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: Th3Issu3]
#23906116 - 12/08/16 09:01 AM (7 years, 1 month ago) |
|
|
Hey guys, I haven't bought the supplies for agar because i had a pretty hefty traffic fine coming up. But today I paid it off and am ordering supplies!!
Also thanks for the advice, I am gonna try to split up that syringe into 5 more syringes as well
-------------------- LF: Gourmet Strains
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: klhouse]
#23906937 - 12/08/16 01:19 PM (7 years, 1 month ago) |
|
|
Quote:
klhouse said: I put in the smallest injection in each jar that I could and NONE of them did anything. I do think you need a certain amount.
Were you flaming between injections? if not left to cool down a lot the first drop of solution could have been killed off. You can easily test this with a spare syringe full of water, flame the needle and put a drop on your hand, takes a few before its cool, depending on how long you leave it after flaming.
You can inject your syringe into a jar of sterile water and then have lots of spore solution to work with. Or you could wait until you have just 1-2ml left and then such sterile water into the syringe, but if that is done repeatedly you keep diluting it down & down.
I have sucked nutrient water into a syringe with no liquid in them and got growth, i.e. there were spores still stuck to the syringe body or rubber plunger and they grew off that.
|
no0bsawse
Yearnin' for Learnin'

Registered: 08/26/16
Posts: 104
Last seen: 5 years, 1 month
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: blackout]
#23907275 - 12/08/16 02:52 PM (7 years, 1 month ago) |
|
|
I have not inoculated yet, but I will as soon as I dilute this syringe, which I will be doing with a jar of sterile water
-------------------- LF: Gourmet Strains
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Can I really use one syringe to innoc 30+ jars? [Re: blackout]
#23907309 - 12/08/16 03:03 PM (7 years, 1 month ago) |
|
|
Quote:
blackout said: I have sucked nutrient water into a syringe with no liquid in them and got growth, i.e. there were spores still stuck to the syringe body or rubber plunger and they grew off that.

sometimes ive had crusted spores inside the syringe. awesome.
|
|