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Offlineno0bsawse
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Registered: 08/26/16
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Can I really use one syringe to innoc 30+ jars?
    #23905616 - 12/08/16 12:33 AM (7 years, 1 month ago)

Hello, I have a spore syringe with half a print inside of it, and is just loaded with spores. Instead of splitting it up into many syringes, could I just squirt literally one drop per innoc hole in my BRF jars and have everything be all good?


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LF: Gourmet Strains


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Invisibler.lutece
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Re: Can I really use one syringe to innoc 30+ jars? [Re: no0bsawse]
    #23905625 - 12/08/16 12:39 AM (7 years, 1 month ago)

The problem with that is that you then have a single inoculation point, which will take much longer to colonize the jar. Also, I seem to remember reading that the fluid from the syringe is beneficial to the germination process, and too little fluid will stunt germination. (I can't verify that, however.)


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One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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Offlinelukehighwalker710
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Re: Can I really use one syringe to innoc 30+ jars? [Re: r.lutece]
    #23905629 - 12/08/16 12:41 AM (7 years, 1 month ago)

Never tried (or read much into) PF tek myself but learn agar and one drop from that syringe could start one plate that could lead to a near endless amount of innoculant.


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:vaped: Highwalker :bongload:


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Invisiblevan hattonFacebookDiscord
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Re: Can I really use one syringe to innoc 30+ jars? [Re: lukehighwalker710]
    #23905630 - 12/08/16 12:43 AM (7 years, 1 month ago)

:whathesaid:


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlineklhouse
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Re: Can I really use one syringe to innoc 30+ jars? [Re: van hatton]
    #23905640 - 12/08/16 12:59 AM (7 years, 1 month ago)

All I know is, I have done jars for years with no problems. Then I thought---There are millions of spores in this syringe, I'll put the tiniest dab in each jar... I put in the smallest injection in each jar that I could and NONE of them did anything. I do think you need a certain amount.


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Shroomery mycologist definitely know their shit. :wink:


Knowledge talks. Wisdom listens.


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Offlinetump
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Re: Can I really use one syringe to innoc 30+ jars? [Re: klhouse]
    #23905722 - 12/08/16 02:59 AM (7 years, 1 month ago)

In theory for cakes its great. Ive divided syringes into 50. Syringes with similar growth rates. with Spores less is more. But you need to still have the water to spread the growth down the cake.


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Offlinewtfcrazymofo
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Re: Can I really use one syringe to innoc 30+ jars? [Re: no0bsawse]
    #23905760 - 12/08/16 03:44 AM (7 years, 1 month ago)

You can make one drop spread out to as many jars as you can handle with some good old agar work.  You might want to throw the pf tek out the window though.


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Offlinespore-ty
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Re: Can I really use one syringe to innoc 30+ jars? [Re: wtfcrazymofo]
    #23905803 - 12/08/16 04:51 AM (7 years, 1 month ago)

Quote:

wtfcrazymofo said:
You can make one drop spread out to as many jars as you can handle with some good old agar work.  You might want to throw the pf tek out the window though.





Yup literally ONE or two single droplets on to each agar plate and you'll be able to do infinite jars and you'll know of the mycelium is actually clean.


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Invisiblefilthyknees
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Re: Can I really use one syringe to innoc 30+ jars? [Re: spore-ty]
    #23905935 - 12/08/16 07:11 AM (7 years, 1 month ago)

I use .5-1cc/ml of spore solution per quart, otherwise ime colonization can take far longer (I've tried the single drop)


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But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineTh3Issu3
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Re: Can I really use one syringe to innoc 30+ jars? [Re: filthyknees]
    #23906015 - 12/08/16 08:02 AM (7 years, 1 month ago)

Why don't you put a small drop on a agar plate, then turn the plate into more plates and use them to do 30 jars.

Than you'll Use your needle to do 30000000 jars that way.


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Offlineno0bsawse
Yearnin' for Learnin'

Registered: 08/26/16
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Re: Can I really use one syringe to innoc 30+ jars? [Re: Th3Issu3]
    #23906116 - 12/08/16 09:01 AM (7 years, 1 month ago)

Hey guys, I haven't bought the supplies for agar because i had a pretty hefty traffic fine coming up. But today I paid it off and am ordering supplies!!

Also thanks for the advice, I am gonna try to split up that syringe into 5 more syringes as well


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LF: Gourmet Strains


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Offlineblackout
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Re: Can I really use one syringe to innoc 30+ jars? [Re: klhouse]
    #23906937 - 12/08/16 01:19 PM (7 years, 1 month ago)

Quote:

klhouse said:
I put in the smallest injection in each jar that I could and NONE of them did anything. I do think you need a certain amount.



Were you flaming between injections? if not left to cool down a lot the first drop of solution could have been killed off. You can easily test this with a spare syringe full of water, flame the needle and put a drop on your hand, takes a few before its cool, depending on how long you leave it after flaming.

You can inject your syringe into a jar of sterile water and then have lots of spore solution to work with. Or you could wait until you have just 1-2ml left and then such sterile water into the syringe, but if that is done repeatedly you keep diluting it down & down.

I have sucked nutrient water into a syringe with no liquid in them and got growth, i.e. there were spores still stuck to the syringe body or rubber plunger and they grew off that.



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Offlineno0bsawse
Yearnin' for Learnin'

Registered: 08/26/16
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Re: Can I really use one syringe to innoc 30+ jars? [Re: blackout]
    #23907275 - 12/08/16 02:52 PM (7 years, 1 month ago)

I have not inoculated yet, but I will as soon as I dilute this syringe, which I will be doing with a jar of sterile water


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LF: Gourmet Strains


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InvisiblemushboyMDiscord
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Re: Can I really use one syringe to innoc 30+ jars? [Re: blackout]
    #23907309 - 12/08/16 03:03 PM (7 years, 1 month ago)

Quote:

blackout said:
I have sucked nutrient water into a syringe with no liquid in them and got growth, i.e. there were spores still stuck to the syringe body or rubber plunger and they grew off that.






:takingnotes: sometimes ive had crusted spores inside the syringe. awesome.


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