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Invisiblewolfedawwg
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First Grow attempt, couple questions...
    #23856880 - 11/22/16 10:40 AM (7 years, 3 months ago)

I am attempting my first grow and just have a couple questions and any advice is also welcome.
So, I made a spore syringe with a Psilocybe cubensis(Martiniue) print.
I then inoculated 3 test jars of WBR just to make sure I had a viable syringe as I'm not so sure I let all of the iso evaporate out of my jar before I scraped in some spores with a flame sterilized scalpel.
I then aspirated sterile water in and out of the jar a couple times.
THIS IS WHERE I FUCKED UP.....
After inoculation I shook the shit out of the jars which I now reaize should only be done with agar or LC inoculation.
So, this is where I'm at now........
Jars were inoculated on 11/14/2016
First sing of growth in 1/3 Jars was on 11/19/2016 (about the size of a kernel of corn), the other 2 jars have no sign of germination yet.
This is that Jar today.....

There are a couple other pea size spots of growth in this jar as well as one about half the size in the pic.

So, my questions are:

1. Did the other 2 jars stall because I shook the shit out of them or can germination times vary using MS?
2. Is that too much condensation?
3. Should I crack the lid a little on these jars? (midnight tonight will be day 8 after inoculation)

I have also been monitoring the temps with a temperature gun and the lowest temp I noted was 69 and the highest being 79 (on the side of the jars), but it's mostly in the mid to low 70's.


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InvisiblemushboyMDiscord
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Re: First Grow attempt, couple questions... [Re: wolfedawwg]
    #23856909 - 11/22/16 10:47 AM (7 years, 3 months ago)

1.i always shake a MS grain jar. or any grain jar...spores have taken a few days to a week or so to germ.

2.condensation isnt a issue. but you have alot. did you allow the wbr to dry?

3.do not crack the lid. you could losen slightly. the lid should have a hole with some filter.(tyvek/poly/sfd/whatever)

what tek did you use. i hope its not 'this and that'

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Invisiblewolfedawwg
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Re: First Grow attempt, couple questions... [Re: mushboy]
    #23856944 - 11/22/16 10:56 AM (7 years, 3 months ago)

Quote:

mushboy said:
2.condensation isnt a issue. but you have alot. did you allow the wbr to dry?




They seemed dry to me, i did the paper towel test and it barely left any water stains on the paper towel......:shrug:
I'm also following a TEK with a no lid modification, GE is obtained by cracking the lid a tiny bit but from what I read it should be fine up until around the 10 day mark without having to crack the lid.


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OfflineChk
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Re: First Grow attempt, couple questions... [Re: wolfedawwg]
    #23857033 - 11/22/16 11:29 AM (7 years, 3 months ago)

Quote:

mushboy said:
So, my questions are:

1. Did the other 2 jars stall because I shook the shit out of them or can germination times vary using MS?
2. Is that too much condensation?
3. Should I crack the lid a little on these jars? (midnight tonight will be day 8 after inoculation)





1-Germination time is very variable, no need to worry before 10 days i guess, but it could take more

2-probably too much condensation, could slow the colonization time.

3-don't crack the lid unless the mycélium already colonized a part of the jar AND is stalling,
really you could colonize a jar that size without any GE if done correctly

4- learn agar !


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Edited by Chk (11/22/16 11:30 AM)

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Invisiblewolfedawwg
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Re: First Grow attempt, couple questions... [Re: Chk]
    #23857107 - 11/22/16 11:55 AM (7 years, 3 months ago)

Quote:

Chk said:
4- learn agar !



Agreed, I spent 2 hours this morning trying to find some agar-agar but I live right on the edge of civilization......lol.
I'm ordering some right now!


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InvisiblemushboyMDiscord
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Re: First Grow attempt, couple questions... [Re: Chk]
    #23857119 - 11/22/16 11:58 AM (7 years, 3 months ago)

Quote:

Chk said:
Quote:

mushboy said:
So, my questions are:

1. Did the other 2 jars stall because I shook the shit out of them or can germination times vary using MS?
2. Is that too much condensation?
3. Should I crack the lid a little on these jars? (midnight tonight will be day 8 after inoculation)






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InvisiblemushboyMDiscord
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Re: First Grow attempt, couple questions... [Re: mushboy]
    #23857124 - 11/22/16 11:59 AM (7 years, 3 months ago)

i didnt say that OP said that :p

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Invisiblewolfedawwg
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Re: First Grow attempt, couple questions... [Re: mushboy]
    #23857134 - 11/22/16 12:02 PM (7 years, 3 months ago)

Quote:

mushboy said:
i didnt say that OP said that :p



:rofl2:


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Offlinemrmazdarx9
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Re: First Grow attempt, couple questions... [Re: mushboy]
    #23857175 - 11/22/16 12:19 PM (7 years, 3 months ago)

Quote:

mushboy said:
i didnt say that OP said that :p



Or did you??? :howyoudoing:


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InvisiblemushboyMDiscord
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Re: First Grow attempt, couple questions... [Re: mrmazdarx9]
    #23857287 - 11/22/16 01:02 PM (7 years, 3 months ago)

Quote:

mushboy said:
Quote:

mrmazdarx9 said:
i didnt say that OP said that :p



Or did you??? :howyoudoing:




:howyoudoing:

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Invisiblewolfedawwg
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Re: First Grow attempt, couple questions... [Re: mushboy]
    #23883857 - 12/01/16 08:14 AM (7 years, 2 months ago)

Day # 17 after MS inoculation.
Should I shake this fucker??



Lid was cracked a cunt hair on 11/28/2016
Top is pretty well colonized as well (75%), just couldn't a get decent pic through the lid.


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Psilocybe quebecensis


Edited by wolfedawwg (12/01/16 08:24 AM)

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OfflineXpandedMind
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Re: First Grow attempt, couple questions... [Re: wolfedawwg]
    #23884105 - 12/01/16 10:15 AM (7 years, 2 months ago)

On my first try I shook them after I innoc'd with a MS syringe and it did take a long time to germinate. I have read that shaking the jars spreads the spores out so they have to travel much farther to germinate, thus taking longer. I now inject down the side of my jars and do not shake them at all. I do see a definite decrease in the time it takes them to germinate. It is also easier to spot the first signs of growth as it generally starts on the outer edge of your seeds where you innoc'd down the side.


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Invisiblewolfedawwg
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Re: First Grow attempt, couple questions... [Re: XpandedMind]
    #23899280 - 12/06/16 08:42 AM (7 years, 2 months ago)

Getting ready to fruit soon, just wondering what y'all think of this set up, I will be able to stack five, 26 quart monos.......


I have a CPU fan mounted up high with some ironed poly in front of it to scrub the air a little and to reduce the air flow as to not dry out the substrate too fast. I also have 5 holes in the bottom of the closet on the opposite side of the fan.
I'm not even sure if I will use this set up yet, or just may leave the doors open but it will allow me to keep more of a constant temperature as I live in an older drafty trailer.

I will have to run the light opposite to the daylight as temps drop to the low 60's in there at night without the light on. Day time temps are manageable with the light off.
My jars have been colonizing in there without any real concerns, just seem a little bit slow but......:shrug:

This is one of my test jars at 22 days after MS inoculation, I'd say it's about 85%-90% colonized.....


Here is 4 more that were knocked up 11 days ago.....


Also knocked up 8 more last night.
Thanks for any and all input, I also take criticism well....:dumblol:

Edited by wolfedawwg (12/07/16 03:54 PM)

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Invisiblewolfedawwg
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Re: First Grow attempt, couple questions... [Re: wolfedawwg]
    #23904068 - 12/07/16 03:56 PM (7 years, 2 months ago)

:bump:


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OfflineKenetic
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Re: First Grow attempt, couple questions... [Re: wolfedawwg]
    #23904752 - 12/07/16 07:19 PM (7 years, 2 months ago)

So you built that big box just to suffocate your shrooms? Looks like it took a while too


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Invisiblewolfedawwg
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Re: First Grow attempt, couple questions... [Re: Kenetic]
    #23904799 - 12/07/16 07:30 PM (7 years, 2 months ago)

:rofl2:, no, its a portable closet, I just put a few holes in it.
It was previously set up for clones, I just modified it a bit.
I lost about 2 hours of my life, minus breaks.....:jah:
The fan will also be constantly cycling air and taking it in from the room outside of the closet...:shrug:


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Psilocybe quebecensis


Edited by wolfedawwg (12/07/16 07:46 PM)

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