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vinsue
Grand Old Fart


Registered: 02/17/04
Posts: 17,953
Loc: The Garden State(NJ)
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clone from dried caps
#2390329 - 03/01/04 06:04 AM (20 years, 3 months ago) |
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Is it possible to start a clone on agar using mushroom sample that was dried and has been frozen for over 1 year? Original sample was extremely potent. Thanks
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"All mushrooms are edible; but some only once." Croatian proverb. BTW ... Have You Rated Ythans Mom Yet ?? ... ... HERE'S HOW ... (be nice) . ...
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Floyd_
Stranger

Registered: 05/18/01
Posts: 207
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Re: clone from dried caps [Re: vinsue]
#2390396 - 03/01/04 07:16 AM (20 years, 3 months ago) |
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unless its a species that can withstand freezing its most assuredly 100% dead. And even if it is a species that can withstand freezing..its still probably dead
-------------------- Dimensions
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Anonymous
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Re: clone from dried caps [Re: Floyd_]
#2390667 - 03/01/04 09:37 AM (20 years, 3 months ago) |
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No clone but you can maybe get some spores to germinate.
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abhi
Why not?



Registered: 10/11/03
Posts: 16,406
Loc: in some small dive
Last seen: 5 months, 12 days
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Re: clone from dried caps [Re: vinsue]
#2406436 - 03/07/04 08:30 PM (20 years, 3 months ago) |
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I wouldn't bet on it, but if you have the resources to spare you may as well try it. You could get lucky.
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Scourge
The Dr.
Registered: 12/03/00
Posts: 293
Loc: Near flint Michgan, USA
Last seen: 18 years, 6 months
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Re: clone from dried caps [Re: ]
#2407038 - 03/08/04 12:40 PM (20 years, 3 months ago) |
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i agree, spore germination would probably be your best bet.
-------------------- "Those who walk in Love and Truth shall grow in Honor and Strength."
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Mitchnast
Toadmonger


Registered: 10/27/99
Posts: 8,657
Loc: Okanagan
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Re: clone from dried caps [Re: Scourge]
#2424848 - 03/12/04 04:19 PM (20 years, 3 months ago) |
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i bet if you tried to clone a dried cap, you would germinate the spores anyway, unless you used peroxide first
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 months
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Re: clone from dried caps [Re: vinsue]
#2438551 - 03/16/04 08:47 AM (20 years, 3 months ago) |
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Dried caps have been known to start growing again on agar. Go for it.
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Anonymous
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Re: clone from dried caps [Re: RogerRabbit]
#2438564 - 03/16/04 08:55 AM (20 years, 3 months ago) |
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What!?!? Thats crazy are you just fucking around or serious. Well any way thats cool!
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CaptainFuture
Finally being here now :)


Registered: 12/31/03
Posts: 889
Loc: Earth
Last seen: 4 days, 1 hour
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Re: clone from dried caps [Re: RogerRabbit]
#2440093 - 03/16/04 03:53 PM (20 years, 3 months ago) |
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NEVER! A dried cap is not a sklerotia...
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snowspeed1
Psychonaut
Registered: 08/27/03
Posts: 117
Last seen: 19 years, 6 months
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Re: clone from dried caps [Re: vinsue]
#2441863 - 03/17/04 01:06 AM (20 years, 3 months ago) |
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Actually i read a post on this board about a guy who did it with a 20 year old mushroom. there was little left but dust. never underestimate the power of a determined nature. he used peroxide on it as well as a safety measure.
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Joshua
Holoman


Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: clone from dried caps [Re: snowspeed1]
#2441891 - 03/17/04 01:19 AM (20 years, 3 months ago) |
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"unless its a species that can withstand freezing its most assuredly 100% dead."
This is true.
Cubensis fruit bodies do not survive dessication. I personally am not aware of any species whos fruitbody will survive dessication, especially for 20 years.
It is probable that what was grown from the cap were spores and not tissue.
Joshua
-------------------- The Shroomery Bookstore Great books for inquiring minds! "Life After Death is Saprophytic!"
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: clone from dried caps [Re: vinsue]
#2442524 - 03/17/04 08:24 AM (20 years, 3 months ago) |
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With all due respect to those who disagree or have never done it, you can take dried mycelium and get it growing again on agar. I've done this many times with dry mushrooms. The stipe works better than the cap. This is not due to spores germinating, but due to the dried mushroom tissue rehydrating and beginning to grow again.
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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ragadinks
MrBeatle


Registered: 10/20/03
Posts: 1,298
Last seen: 8 months, 17 days
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Re: clone from dried caps [Re: RogerRabbit]
#2442632 - 03/17/04 08:57 AM (20 years, 3 months ago) |
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Does that depend on the species? With what species did you try that out? Did you just put them on agar or did you rehydrate them first?
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Newdude1
Stranger
Registered: 02/08/04
Posts: 11
Last seen: 19 years, 8 months
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Re: clone from dried caps [Re: RogerRabbit]
#2442642 - 03/17/04 08:59 AM (20 years, 3 months ago) |
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Seems to be some debate on this, I think maybe it calls for an experiment with pictures RodgerRabbit? mabye some dried myc, dried fruit, and dried cap spores, that would be really cool.
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ShroomInhaler
No, I don'tsmoke them! :P

Registered: 03/03/04
Posts: 102
Last seen: 17 years, 3 months
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Re: clone from dried caps [Re: Newdude1]
#2443106 - 03/17/04 11:43 AM (20 years, 3 months ago) |
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So what some of you are saying is that if i purshase dry mushrooms from someone, I don't need a spore syringe or anything, I can just try and use the dried msuhrooms??
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PooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
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I'll bet there were spores on the stem, causing germination of more mycelium..
But that's just my dumb ass opinion !
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----------- Sniff, Sniff... What's that smell???... ohhhhh.!! ------------------ Pot Free for another : nevermind.. never made it..
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Joshua
Holoman


Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: clone from dried caps [Re: RogerRabbit]
#2443463 - 03/17/04 01:16 PM (20 years, 3 months ago) |
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This is news to me! I'll try it and see what happens.
Joshua
-------------------- The Shroomery Bookstore Great books for inquiring minds! "Life After Death is Saprophytic!"
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Anonymous
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Re: clone from dried caps [Re: Joshua]
#2443712 - 03/17/04 02:09 PM (20 years, 3 months ago) |
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Ok well though I am skeptic. I do believe that this could be true. Yeast a cusin of fungi, does reanimate when dried and rehydrated. So I guess this could be true for mushrooms. As long as the cell walls arnt damaged in the drying process. Hmmmm
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Joshua
Holoman


Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: clone from dried caps [Re: ]
#2447599 - 03/18/04 02:43 PM (20 years, 3 months ago) |
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Surely someone else has knowledge on the subject!
Joshua
-------------------- The Shroomery Bookstore Great books for inquiring minds! "Life After Death is Saprophytic!"
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bobmarley4prez

Registered: 10/20/03
Posts: 87
Loc: southwest
Last seen: 11 years, 4 months
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Re: clone from dried caps [Re: ]
#2447614 - 03/18/04 02:48 PM (20 years, 3 months ago) |
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Yeast a cusin of fungi
yeast is a term used to describe a unicellular fungi of three of the four phyla of fungi (zygomycota, ascomycota, and basidiomycota(most mushrooms). Peace
-------------------- Anything above this line is a lie. i do not cultivate psychadelic mushrooms nore do I intend to. One day I encountered a bufo toad. Since that day I have been instilled with the power of the devil.
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