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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Pre-sealing filter patch bags before PC
    #23900434 - 12/06/16 04:25 PM (7 years, 1 month ago)

I've scoured the search function and can't find a consensus on pre-sealing filter patch bags before pressure cooking.  I have a SAB, but allowing a plenum of air sounds like an invite for contaminants.  Flow hood is out of the question.  I'd like to pre-seal the bags before pressure cooking. From what I've gathered it's possible, but you want the filter patch to be right on top of the substrate so that the bags don't explode.  Then hang the bags up to allow air in through the filter patch.

I've purchased some large 5 micron filter patch bags, but unfortunately the patch is really high up on the bags, making it seem impossible to have the patch laying on top of the substrate.  Any ideas for solutions? 

Spoon for size reference :cool:



Will be inoculating these bags with LC made from agar


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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Pre-sealing filter patch bags before PC [Re: Sirtalis]
    #23900442 - 12/06/16 04:28 PM (7 years, 1 month ago)

Dude, I can't get a size comparison with that fork...where's the spoon?

I seal them and fold them over with the patch on the inside of the fold.


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Pre-sealing filter patch bags before PC [Re: Mycolorado]
    #23900451 - 12/06/16 04:31 PM (7 years, 1 month ago)

Lol dammit.  Thanks for your reply.  New to spawn bags and the thought of sealing them gives me mild anxiety.


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Pre-sealing filter patch bags before PC [Re: Sirtalis]
    #23900617 - 12/06/16 05:22 PM (7 years, 1 month ago)

Can you pre-seal .2 micron bags?  Thinking of buying smaller bags and smaller air filter.  Apparently trich can get into 5 micron bags.


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InvisibleMarty Mycfly
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Registered: 12/16/13
Posts: 976
Re: Pre-sealing filter patch bags before PC [Re: Sirtalis]
    #23900748 - 12/06/16 05:59 PM (7 years, 1 month ago)

I use the 5 micron for both sub and spawn and have never had a problem.


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Pre-sealing filter patch bags before PC [Re: Marty Mycfly]
    #23900806 - 12/06/16 06:14 PM (7 years, 1 month ago)

:awedancexmas:  Awesome, thank you!


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Invisibleazur
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Pre-sealing filter patch bags before PC [Re: Sirtalis]
    #23901038 - 12/06/16 07:19 PM (7 years, 1 month ago)

I use .5 and do not preseal. I don't like having to cut them open to seal again. One more unnecessary vector...


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FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Pre-sealing filter patch bags before PC [Re: azur]
    #23901046 - 12/06/16 07:20 PM (7 years, 1 month ago)

I won't be cutting them open after PCing.  I am going to be injecting LC through a SHIP instead of going g2g.


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Invisibleazur
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Pre-sealing filter patch bags before PC [Re: Sirtalis]
    #23901059 - 12/06/16 07:23 PM (7 years, 1 month ago)

Oh. Rock on then.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Pre-sealing filter patch bags before PC [Re: azur]
    #23901097 - 12/06/16 07:32 PM (7 years, 1 month ago)

Quote:

azur said:
Oh. Rock on then.




:raisemyglass:


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